Study on the DNA Barcoding of Genus Ligularia Cass. (Asteraceae)

doi:10.7677/ynzwyj201515082

Abstract

Abstract:

DNA barcoding is a new technology which can identify species rapidly based on short and standardized DNA sequences. Ligularia, a genus of Asteraceae with about 140 species, exhibits high morphological and ecological diversity, which makes the classification and species delimitation difficult, especially in the cases of closely related taxa. In this study, we tested four DNA core barcoding regions (ITS, matK, psbAtrnH and rbcL) in 144 samples representing 35 species of Ligularia. The results revealed that the chloroplast regions (matK, psbAtrnH and rbcL) have extremely low species identification rate due to low interspecific variation. Conversely, ITS sequence showed higher species identification rate (60%) and could discriminate the species which are difficult to identify. The combination of these four gene fragments did not improve the ability of species discrimination.

Key words: DNA barcoding, Ligularia, species identification, ITS, matK, psbA-trnH, rbcL

摘要：

DNA条形码是一项利用短的、标准的DNA片段对物种进行快速、有效识别和鉴定的新技术。菊科橐吾属约140种，是典型的高山植物，种间杂交频繁，形态变异复杂，从形态学方面鉴定近缘种较为困难。本研究选取4个DNA核心条形码片段（ITS，matK，psbAtrnH和rbcL），对橐吾属35种144个个体进行条形码研究。研究结果显示叶绿体基因matK，psbAtrnH和rbcL在种内和种间变异都很小，对橐吾属的物种鉴定率极低；ITS在种间变异率相对较大，物种鉴定率为60%。而各片段联合后的物种鉴定率并未提高。

关键词: DNA条形码, 橐吾属, 物种鉴别, ITS, matK, psbA-trnH, rbcL

CLC Number:

Q 949，Q 781

HE Wei-Ying-, PAN Yue-Zhi. Study on the DNA Barcoding of Genus Ligularia Cass. (Asteraceae)[J]. Plant Diversity, 2015, 37(06): 693-703.

何维颖1、2、3, 潘跃芝1、2. 菊科橐吾属植物的DNA条形码研究[J]. Plant Diversity, 2015, 37(06): 693-703.

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