Physiological Responses of Betula halophila (Betulaceae) to Salt Stress

doi:10.3724 SP.J.1143.2009.08221

Plant Diversity ›› 2009, Vol. 31 ›› Issue (03): 260-264.DOI: 10.3724 SP.J.1143.2009.08221

• Articles • Previous Articles Next Articles

Physiological Responses of Betula halophila (Betulaceae) to Salt Stress

ZHANG Hai-Bo, ZENG You-Ling, LAN Hai-Yan , ZHANG Fu-Chun

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering , College of Life Science and Technology , Xinjiang University , Urumqi 830046 , China

Received:2008-12-10 Online:2009-06-25 Published:2009-06-25

盐胁迫下盐桦生理响应的变化分析

张海波, 曾幼玲, 兰海燕, 张富春

新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室, 新疆乌鲁木齐 830046

Abstract

Abstract: The physiological responses and the relevant anatomical structure of Betula halophila under salt stress were assayed. Results showed that the relative water and proline contents of the leaf were gradually declined, while the MDA content was correlated with the CAT activity in the leaf under salt stress. The CAT activity was ascended responding to the stability of MDA content at 50 - 200 mmol/L NaCl , but it was decreased at 300mmol/L NaCl , which caused the elevation of the MDA. The K⁺􊄯Na ⁺ ratios in the leaf and root were declined under salt stress, while the K⁺ content was always higher than Na ⁺ content in leaf. Crystals of Betula halophila containing the C, O and Ca element were discovered in the stem and leaf, but not in root, and will be further confirmed by chemical experiment related to cell components .

Key words: Betula halophila

摘要： 对组织培养获得的盐桦( Belula halophila ) 苗在盐胁迫下的生理指标和解剖结构进行了分析, 结果显示, 随着盐浓度的增加, 植物叶片相对含水量逐渐降低; 脯氨酸( Pro) 含量逐渐增加; 叶片丙二醛(MDA) 含量和过氧化氢酶(CAT) 活性大小存在相关性, 在50～200 mmol/L 盐胁迫下, 植物的CAT 活性是递增的, 200 mmol/L NaCl 处理时达到最高, 同时叶片MDA 含量在50～200 mmol/L 盐处理时变化不明显;CAT 活性在300mmol/L NaCl 处理时突然降低, 此时叶片MDA 含量大; 植物叶片和根的离子含量测定表明,在盐胁迫下K+􊄯Na+ 比值逐渐降低, 叶片中K⁺ 含量始终高于Na ⁺ 含量; 石蜡切片和扫描电镜发现盐桦茎、叶中有晶体状物质存在, 通过X-ray 分析表明这种晶体含有C, O, Ca 元素, 相关的细胞成分化学实验进一步确定其结晶体的成分。

关键词: 盐桦, 脯氨酸, 丙二醛, 过氧化氢酶

CLC Number:

Q 945

ZHANG Hai-Bo, ZENG You-Ling, LAN Hai-Yan , ZHANG Fu-Chun. Physiological Responses of Betula halophila (Betulaceae) to Salt Stress[J]. Plant Diversity, 2009, 31(03): 260-264.

张海波, 曾幼玲, 兰海燕, 张富春 . 盐胁迫下盐桦生理响应的变化分析[J]. Plant Diversity, 2009, 31(03): 260-264.

[1]	LUO JingDe, GAN XiaoHong, JIA XiaoJuan, XIE Dan, ZHANG Lie. Biological Characteristics of Seeds of Endangered Plant Tetracentron sinense （Tetracentraceae） [J]. Plant Diversity, 2010, 32(03): 204-210.
[2]	DU Yan, YU DiQiu. Effect of β-Aminobutyrie Acid on Anthocyanin of Leaves of Arabidopsis thaliana (Cruciferae) [J]. Plant Diversity, 2010, 32(03): 263-269.
[3]	XIAO YueE, TIAN Qi, ZHOU XiangYu, CHEN XiaoYa, HU YongHong. Reproductive Ecology of Iris ensata (Iridaceae) [J]. Plant Diversity, 2010, 32(02): 93-102.
[4]	ZHANG XiuJun, JIANG PiWen，DONG Dan, XIA ZongWei. Research Advances in Methane Emission from Plants [J]. Plant Diversity, 2010, 32(02): 177-182.
[5]	WANG WeiQing , ,CHENG Xiao*, JIAO Yu ,CHEN GuiJu. Substratum Affects the Gametophyte Development and Reproduction of Sinopteris grevilleoides(Sinopteridaceae), an Endangered Fern Endemic to China [J]. Plant Diversity, 2010, 32(01): 25-31.
[6]	HAN ChunYan, , LONG ChunLin. Seed Dormancy, Germination and Storage Behavior of Magnolia wilsonii (Magnoliaceae), an Endangered Plant in China [J]. Plant Diversity, 2010, 32(01): 47-52.
[7]	ZHOU Qiang, YU Bing-Jun. Changes in Free, Conjugated and Bound Polyamine Content in Salt Adaptation of Vetiver Grass (Vetiveria zizanioides , Poaceae) [J]. Plant Diversity, 2009, 31(06): 477-485.
[8]	YU Zhao-Duan, WANG Li-Na , CAO Chen-Xing^** , HU Xiang-Yang^,. The Effects of Exogenous Nitric Oxide on Growth, Active Oxygen Metabolism and Photosynthetic Characteristics in Cucumber (Cucumis sativus) Seedlings Under Cadmium Stress [J]. Plant Diversity, 2009, 31(06): 486-492.
[9]	YANG Feng-Xi^, , YU Di-Qiu. Overexpression of Arabidopsis MiR396 Enhances Drought Tolerance in Transgenic Tobacco Plants [J]. Plant Diversity, 2009, 31(05): 421-426.
[10]	JIANG Xue-Mei , HU Jin-Yao , QI Wen-Hua, CHEN Guang-Deng , XU Xiao. Different Physiological Responses of Male and Female Ginkgo biloba (Ginkgoaceae) Seedlings to Salt Stress [J]. Plant Diversity, 2009, 31(05): 447-453.
[11]	JIANG Wen-Bo^, , YU Di-Qiu. Arabidopsis WRKY2 Transcription Factor may be Involved in Osmotic Stress Response [J]. Plant Diversity, 2009, 31(05): 427-432.
[12]	TIAN Xue-Jun , TAO Hong-Zheng , LUO Jing, ZHANG Xu-Dong , TAO Fa-Qing. Physiological Effect of Heat Stress on Pea (Pisum sativum) Hypocotyls [J]. Plant Diversity, 2009, 31(04): 363-368.
[13]	YANG Jun , YANG Yun-Qiang, YANG Yong-Ping , HU Xiang-Yang. The Sample Preparation and the Improvement Method of Two-Dimensional Electrophoresis of Proteins from Antiaris toxicaria (Moraceae) Seed [J]. Plant Diversity, 2009, 31(04): 357-362.
[14]	LIU Dong-Me^{i ,} , YANG Feng-Xi^, , YU Di-Qiu . Overexpression of miR396 miRNAs Caused Flower Stigma Curved in Arabidopsis thaliana [J]. Plant Diversity, 2009, 31(04): 353-356.
[15]	LIU Run-Hua^, , JIANG Wen-Bo ^, , YU Di-Qiu. The Response of AtCER to Oxidative Stress in Arabidopsis thaliana [J]. Plant Diversity, 2009, 31(04): 326-334.

Physiological Responses of Betula halophila (Betulaceae) to Salt Stress

盐胁迫下盐桦生理响应的变化分析

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments