Effect of β-Aminobutyrie Acid on Anthocyanin of Leaves of Arabidopsis thaliana (Cruciferae)

doi:10.3724/SP.J.1143.2010.09249

Plant Diversity ›› 2010, Vol. 32 ›› Issue (03): 263-269.DOI: 10.3724/SP.J.1143.2010.09249

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Effect of β-Aminobutyrie Acid on Anthocyanin of Leaves
of Arabidopsis thaliana (Cruciferae)

DU Yan1,2, YU DiQiu1

1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China;
2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2009-12-11 Online:2010-09-06 Published:2010-06-25
Contact: YU DiQiu

β-氨基丁酸对拟南芥叶片花色素苷的影响

杜艳1,2，余迪求1

1 中国科学院西双版纳热带植物园，云南昆明650223；2 中国科学院研究生院，北京100049

通讯作者: 余迪求

Abstract

Abstract: To analyze the effect of βaminobutyrie acid (BABA) on anthocyanin of leaves of Arabidopsis, 30old plants were sprayed with BABA while the control were sprayed with water. After treated with BABA, the content of anthocyanin was significantly lower than that of control. Furthermore, the results from RTPCR showed that CHS, LDOX, UF3GT were down regulated compared with contro1, while PAL showed an opposite trend. At the same time, the activity of PPO, which played an important role in the degradation of anthocyanin, showed higher level than control. In addition, the antioxidant capacity, the death rate of cells and electrical conductivity of leaves were also decreased with BABA treatment. All results suggested that BABA might inhibit the accumulation of anthocyanin in leaves of Arabidopsis in vitro.

Key words: β-aminobutyric acid

摘要： 研究发现β氨基丁酸（BABA）处理减少了拟南芥（Arabidopsis thaliana）叶片花色素苷的积累, 而且BABA处理降低了花色素苷合成基因(CHS,LDOX,UF3GT)的表达，却上调了苯丙氨酸解氨酶（PAL）的表达，同时促进了花色素苷降解酶多酚氧化酶（PPO）的活性。叶片对DPPH(1, 1二苯基2苦基苯肼)的清除能力、总酚和类黄酮含量以及电导率和细胞死亡率的测定表明BABA降低了叶片的抗氧化能力、电导率及细胞死亡率。结果表明BABA处理抑制了花色素苷在叶片中的积累。

关键词: β-氨基丁酸；花色素苷；拟南芥

CLC Number:

Q 945

DU Yan, YU DiQiu. Effect of β-Aminobutyrie Acid on Anthocyanin of Leaves
of Arabidopsis thaliana (Cruciferae)[J]. Plant Diversity, 2010, 32(03): 263-269.

杜艳1,2，余迪求1 . β-氨基丁酸对拟南芥叶片花色素苷的影响[J]. Plant Diversity, 2010, 32(03): 263-269.

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Effect of β-Aminobutyrie Acid on Anthocyanin of Leaves
of Arabidopsis thaliana (Cruciferae)

β-氨基丁酸对拟南芥叶片花色素苷的影响

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