Molecular Phylogeny of Ten Species of Oxytropis Based on psbAtrnH Sequences

doi:10.7677/ynzwyj201413135

Abstract

Abstract:

Total DNA from 56 individual plants collected in 28 sample sites of 10 Oxytropis species in Inner Mongolia was extracted in this research, and the psbAtrnH sequence was amplied and sequenced. Then the sequences were analyzed by ClustalX and MEGA 50. The phylogenetic trees were constructed by Maximum Likelihood methods in MEGA 50. The results showed as follows: (1) There were 50 variable sites, 36 informative site and 34% of the interspecific difference in the 10 Oxytropis species, and the GC contents of the psbAtrnH region were between 2318%~2572%. (2) The 10 species of Oxytropis were probably monophyletic, clearly separating from the Astragalus species and the bootstrap value was 99%. (3) The samples of Oxytropis glabra var. tenuis and Oglabra clustered a group independently, thus supported the opinion of treating Oxytropis glabra var. tenuis as a variant of Oglabra. (4)The samples of Overticillaris, Oracemosa and Oochrantha mixed. Therefore they showed a close relationship. We are congruent with the points in Flora of Intromongolica, the consensus putting the three species into Subgen. Euoxytropis Sect. Baicalia Stell. ex Bunge. (5) The samples of Ociliata and Oleptophylla clustered a group, which also supported the consensus which put them into sect. Xerobia Bunge. The investigation results indicated that psbAtrnH sequences provided the molecular evidences for the study of the phylogenetic relationships in the Oxytropis at subgenus, sects and species levels.

Key words: Oxytropis, Phylogenetic, psbA-trnH

摘要：

选择内蒙古28个样地采集的10种棘豆属植物56个单株，提取样品的基因组DNA，对其叶绿体psbAtrnH序列进行扩增、测序，所得序列利用ClustalX软件进行对位排列，并用MEGA50软件采用最大似然法构建系统发育树。结果显示：（1）10种棘豆属psbAtrnH序列的变异位点50个，信息位点36个，种间碱基差异百分率为34%，GC含量变化范围在2318%~2572%之间。（2）棘豆属与黄芪属各为一支，自展支持率达99%，这10种棘豆属植物可能为单系起源。（3）系统树中小叶小花棘豆与小花棘豆的样本独立成一支，支持将小叶小花棘豆作为小花棘豆的变种来处理。（4）多叶棘豆、砂珍棘豆和黄毛棘豆的样本相互混杂，表明亲缘关系很近，支持《内蒙古植物志》将三者归入真棘豆亚属轮叶棘豆组的观点。（5）缘毛棘豆与薄叶棘豆的样本聚成一支，支持将二者归入矮生棘豆组。研究表明，psbAtrnH序列可为棘豆属下种间系统发育关系研究提供分子证据。

关键词: 棘豆属, 系统发育, psbA-trnH

CLC Number:

Q 781
Q 949

Lu Ping, GAO Li-Xia, JIN Feng, EN He-Ba-Ya-Er. Molecular Phylogeny of Ten Species of Oxytropis Based on psbAtrnH Sequences[J]. Plant Diversity, 2014, 36(03): 279-284.

卢萍, 高利霞, 金凤, 恩和巴雅尔. 基于psbAtrnH序列的十种棘豆属植物分子系统学研究[J]. Plant Diversity, 2014, 36(03): 279-284.

References

顾红雅, 瞿礼嘉, 1998. 植物分子生物学实验手册［M］. 北京: 高等教育出版社, 4—6
马毓泉, 1989. 内蒙古植物志（第2卷第3分册）［M］. 呼和浩特: 内蒙古人民出版社, 299—331
王关林, 方宏筠, 1998. 植物基因工程原理与技术［M］. 北京: 科学出版社, 598—602
曾建飞, 1998. 中国植物志［M］. 北京: 科学出版社, 42 (2): 9—139
张宏达, 黄云晖, 繆汝槐等, 2004. 种子植物系统学［M］. 北京: 科学出版社, 280—281
Gao J (高静), Lu P (卢萍), Wang JN (王金妞) et al., 2009. Molecular phylogeny of several species of Oxytropis DC. based on 58SrDNA/ITS sequence［J］. Acta Agriculturae BorealiSinica (华北农学报), 24 (6): 168—173
Gao LX (高利霞), Lu P (卢萍), Jin F (金凤) et al., 2013. trnLF sequences analysis and molecular phylogeny of 10 species of Oxytropis［J］. Acta Botanica BorealiOccidentalia Sinica (西北植物学报), 33 (2): 266—271
Han YY (韩艳艳), 2007. Phylogenetic analysis of Zygophyllaceae based on nuclear ITS DNA and chloroplast trnLF sequences［D］. Master’s Thesis of XinJang University (新疆大学硕士论文), 13—21
Jorgensen JL, Stehlik I, 2003. Implications of ITS sequences and RAPD markers for the taxonomy and biogeography of the Oxytropis campestris and Oarctica (Fabaceae) complexes in Alaska［J］. American Journal of Botany, 90 (10): 1470—1480
Kress WJ, Wurdack KJ, Zimmer EA et al., 2005. Use of DNA barcodes to identify flowering plants ［J］. Proceedings of the National Academy of Sciences, 102 (23): 8369—8374
Li YX (李云霞), Lan FR (兰芙蓉), Chang ZY (常朝阳) et al., 2011. Molecular phylogeny of Oxytropis DC. of QinghaiTibetan Plateau by ITS and trnLF sequences ［J］. Journal of Northwest A&F University (西北农林科技大学学报), 39 (11): 187—193
Liu L (刘丽), Zhao YZ (赵一之), 1996. A preliminary study on the species of Oxytropis in NeiMonggol by cladistic taxonomic method［J］. Acta Scientiarum Naturalium Universitatis NeiMonggol (内蒙古大学学报自然科学版), 27 (1): 72—82
Lu P (卢萍), Gao J (高静), Wang JN (王金妞) et al., 2011. Molecular phylogentic analysis of several Oxytropis DC. species in inner Mongolia based on 58SrDNA/ITS sequences［J］. Acta Botanica BorealiOccidentalia Sinica (西北植物学报), 31 (12): 2420—2428
Morden CW, Golden S, 1989. psbA genes indicate common ancestry of prochlorophytes and chloroplasts［J］. Nature, 337: 382—385
Sang T, Crawford DJ, Stuessy TF, 1997. Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae) ［J］. American Journal of Botany, 84: 1120—1136
Umesono K, Inokuchi H, Shiki Y et al.,1988. Structure and organization of Marchantia polymorpha chloroplast genome II Gene organization of the large single copy region from rps12 to atpB［J］. Journal of Molecular Biology, 203 (2): 299—331
Wang JN (王金妞), 2005. The taxonomical study of Oxytropis DC. in inner Mongolia［D］. Master’s Thesis of Inner Mongoia Normal University (内蒙古师范大学硕士论文), 1—69Zhang DJ (张得钧), Gao QB (高庆波), Li FA (李福安) et al., 2011. Study on the chloroplast psbAtrnH nucleotide variation and genetic differentiation in cultivated plants of Gentiana officinalis from Qinghai［J］. Journal of Anhui Agricultural Sciences (安徽农业科学), 39 (25): 15215—15217植物分类与资源学报 2014, 36(3)：285~295

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