分析拟南芥叶片在激素促进的衰老中内源激素变化来解析抑制磷脂酶Dα1延缓激素促进衰老的机制

doi:10.7677/ynzwyj201413220

摘要/Abstract

摘要：

采后衰老进程在很大程度上受到内源和外源激素的影响。抑制拟南芥中磷脂酶Dα1 (phospholipase Dα1, PLDα1)的表达后，使得外源脱落酸（abscisic acid，ABA）和乙烯加速的离体叶片衰老过程在一定程度上得到了缓解。然而，内源激素在这个过程中的作用尚不清楚。本研究对比分析了野生型和PLDα1缺失型两种基因型拟南芥叶片在3种不同人工老化过程中（离体诱导的、外源ABA和乙烯促进的衰老过程），内源ABA，茉莉酸甲酯（methyl jasmonate，MeJA）、吲哚乙酸（indole3acetic acid，IAA）、玉米素核苷（zeatin riboside，ZR）和赤霉素（gibberellic acid，GA3）的含量变化。这5种激素对3种不同衰老处理方式的响应模式表明了人工老化过程存在着两个不同阶段，并且在衰老早期每种激素的变化模式相同。PLDα1功能缺失使得激素加速的衰老过程得以延缓，这与内源ABA、MeJA、ZR和IAA的含量变化有关，而与GA3的含量变化无关。同时，ZR和IAA的变化模式也说明了这两种激素的变化可能是缺失PLDα1延缓激素加速的衰老过程这一事件的原因而非结果。

关键词: 叶片衰老, 采后生理, 激素, 磷脂酶D&alpha, 1, 拟南芥

Abstract:

Postharvest senescence is affected greatly by endogenous and exogenous hormones. The promotive effects of exogenous abscisic acid (ABA) and ethylene on senescence are retarded by suppression of phospholipase Dα1 (PLDα1) in detached Arabidopsis leaves. However, understanding about the roles of endogenous hormones in the retardation of postharvest senescence by the suppression of PLDα1 activity remains incomplete. Here we report changes in the levels of endogenous ABA, methyl jasmonate (MeJA), indole3acetic acid (IAA), zeatin riboside (ZR), and gibberellic acid (GA3) during leaf senescence induced by detachment and also accelerated by the application of exogenous ABA or ethylene in wildtype and PLDα1deficient Arabidopsis leaves. The responses of the five hormones to the three treatments showed the existence of two stages during the artificial senescence, and, for each hormone, the pattern during the early stage was identical. The retardation of senescence in PLDα1deficient plants was associated with changes in ABA, MeJA, ZR, and IAA levels, but was independent of changes in endogenous levels of GA3. The profiles of ZR and IAA suggest that changes in ZR and IAA levels might cause, and not simply be the consequence of, the retardation of senescence that is caused by suppression of PLDα1.

Key words: Leaf senescence, Postharvest physiology, Hormone, Phospholipase Dα1, Arabidopsis thaliana

中图分类号:

Q 945

贾艳霞, 陶发清, 李唯奇. 分析拟南芥叶片在激素促进的衰老中内源激素变化来解析抑制磷脂酶Dα1延缓激素促进衰老的机制[J]. Plant Diversity, 2014, 36(04): 485-496.

JIA Yan-Xia, TAO Fa-Qing, LI Wei-Qi. Analysis of Endogenous Hormone Levels to Reveal the Retardation by Suppression of Phospholipase Dα1 in Arabidopsis Leaves during Hormonepromoted Senescence[J]. Plant Diversity, 2014, 36(04): 485-496.

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