Epigenetic control on transcription of vernalization genes and whole-genome gene expression profile induced by vernalization in common wheat

doi:10.1016/j.pld.2024.02.005

Plant Diversity ›› 2024, Vol. 46 ›› Issue (03): 386-394.DOI: 10.1016/j.pld.2024.02.005

Epigenetic control on transcription of vernalization genes and whole-genome gene expression profile induced by vernalization in common wheat

Yunzhen Li, Liujie Jin, Xinyu Liu, Chao He, Siteng Bi, Sulaiman Saeed, Wenhao Yan

National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China

收稿日期:2023-10-10 修回日期:2024-01-10 出版日期:2024-05-25 发布日期:2024-05-20
通讯作者: Wenhao Yan,E-mail:yanwenhao@mail.hzau.edu.cn
基金资助:
This work was supported by Project 2662020ZKPY002 supported by the Fundamental Research Funds for the Central Universities.

Epigenetic control on transcription of vernalization genes and whole-genome gene expression profile induced by vernalization in common wheat

Yunzhen Li, Liujie Jin, Xinyu Liu, Chao He, Siteng Bi, Sulaiman Saeed, Wenhao Yan

National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China

Received:2023-10-10 Revised:2024-01-10 Online:2024-05-25 Published:2024-05-20
Contact: Wenhao Yan,E-mail:yanwenhao@mail.hzau.edu.cn
Supported by:
This work was supported by Project 2662020ZKPY002 supported by the Fundamental Research Funds for the Central Universities.

摘要/Abstract

摘要： Vernalization is necessary for winter wheat to flower. However, it is unclear whether vernalization is also required for spring wheat, which is frequently sown in fall, and what molecular mechanisms underlie the vernalization response in wheat varieties. In this study, we examined the molecular mechanisms that regulate vernalization response in winter and spring wheat varieties. For this purpose, we determined how major vernalization genes (VRN1, VRN2, and VRN3) respond to vernalization in these varieties and whether modifications to histones play a role in changes in gene expression. We also identified genes that are differentially regulated in response to vernalization in winter and spring wheat varieties. We found that in winter wheat, but not in spring wheat, VRN1 expression decreases when returned to warm temperature following vernalization. This finding may be associated with differences between spring and winter wheat in the levels of tri-methylation of lysine 27 on histone H3 (H3K27me3) and tri-methylation of lysine 4 on histone H3 (H3K4me3) at the VRN1 gene. Analysis of winter wheat transcriptomes before and after vernalization revealed that vernalization influences the expression of several genes, including those involved in leucine catabolism, cysteine biosynthesis, and flavonoid biosynthesis. These findings provide new candidates for further study on the mechanism of vernalization regulation in wheat.

关键词: Wheat, Vernalization, VRN1, Histone modification, Regulate network

Abstract: Vernalization is necessary for winter wheat to flower. However, it is unclear whether vernalization is also required for spring wheat, which is frequently sown in fall, and what molecular mechanisms underlie the vernalization response in wheat varieties. In this study, we examined the molecular mechanisms that regulate vernalization response in winter and spring wheat varieties. For this purpose, we determined how major vernalization genes (VRN1, VRN2, and VRN3) respond to vernalization in these varieties and whether modifications to histones play a role in changes in gene expression. We also identified genes that are differentially regulated in response to vernalization in winter and spring wheat varieties. We found that in winter wheat, but not in spring wheat, VRN1 expression decreases when returned to warm temperature following vernalization. This finding may be associated with differences between spring and winter wheat in the levels of tri-methylation of lysine 27 on histone H3 (H3K27me3) and tri-methylation of lysine 4 on histone H3 (H3K4me3) at the VRN1 gene. Analysis of winter wheat transcriptomes before and after vernalization revealed that vernalization influences the expression of several genes, including those involved in leucine catabolism, cysteine biosynthesis, and flavonoid biosynthesis. These findings provide new candidates for further study on the mechanism of vernalization regulation in wheat.

Key words: Wheat, Vernalization, VRN1, Histone modification, Regulate network

Yunzhen Li, Liujie Jin, Xinyu Liu, Chao He, Siteng Bi, Sulaiman Saeed, Wenhao Yan. Epigenetic control on transcription of vernalization genes and whole-genome gene expression profile induced by vernalization in common wheat[J]. Plant Diversity, 2024, 46(03): 386-394.

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Epigenetic control on transcription of vernalization genes and whole-genome gene expression profile induced by vernalization in common wheat

Epigenetic control on transcription of vernalization genes and whole-genome gene expression profile induced by vernalization in common wheat

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