Plant Diversity ›› 2013, Vol. 35 ›› Issue (5): 547-554.DOI: 10.7677/ynzwyj201312148
欧阳铖人1、2, 孙航2, 李志敏1**, 张建文2
收稿日期:
2012-11-08
出版日期:
2013-09-25
发布日期:
2013-03-20
基金资助:
中科院大科学装置工程项目 (2009LSFGBOWS01)、国家自然科学基金项目 (31061160184)
OU Yang-Cheng-Ren-1、2, SUN Hang-2, LI Zhi-Min-1**, ZHANG Jian-Wen-2
Received:
2012-11-08
Online:
2013-09-25
Published:
2013-03-20
摘要:
根据形态特征难以准确地辨别金合欢属植物,DNA条形码技术提供了一种准确地鉴定物种的方法。本文利用条形码技术对中国金合欢属物种的序列(psbAtrnH、matK、rbcL和ITS)及其不同组合进行比较,通过计算种内和种间变异进行barcoding gap分析,运用Wilcoxon秩和检验比较不同序列的变异性,构建系统树。结果表明:4个片段均存在barcoding gap,ITS序列种间变异率较psbAtrnH、rbcL和matK序列有明显优势,单片段ITS正确鉴定率最高,ITS+rbcL片段联合条码的正确鉴定率最高,因此我们认为ITS片段或条形码组合ITS+rbcL是金合欢属的快速鉴别最理想的条码。
中图分类号:
欧阳铖人1、2, 孙航2, 李志敏1**, 张建文2. DNA条形码在中国金合欢属中的应用[J]. Plant Diversity, 2013, 35(5): 547-554.
OU Yang-Cheng-Ren-, SUN Hang-, LI Zhi-Min-**, ZHANG Jian-Wen. Application of DNA Barcoding in Chinese Acacia s.l. (Leguminosae)[J]. Plant Diversity, 2013, 35(5): 547-554.
Chen SL, Yao H, Han JP et al., 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species[J]. PloS One, 5: e8613 |
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