蒟蒻薯属 (薯蓣科) 植物DNA条形码研究

doi:10.3724/SP.J.1143.2011.11060

摘要/Abstract

摘要：

蒟蒻薯属（Tacca）植物种间在形态上差别不大，导致分类上存在一定的困难。DNA条形码是一种利用短的DNA标准片段来鉴别和发现物种的方法。本研究利用核基因ITS片段和叶绿体基因trnHpsbA, rbcL, matK片段对蒟蒻薯属6个种的DNA条形码进行研究,对4个DNA片段可用性，种内种间变异，barcode gap进行了分析，采用Treebased和BBA两种方法比较不同序列的鉴定能力。结果显示：单片段ITS正确鉴定率最高，片段组合rbcL+matK正确鉴定率最高。支持CBOL植物工作组推荐的条码组合rbcL+matK可作为蒟蒻薯属物种鉴定的标准条码，建议ITS片段作为候选条码。丝须蒟蒻薯Tacca integrifolia采自西藏的居群与马来西亚居群形成了2个不同的遗传分支，且两者在形态上也存在一定的差异，很可能是一个新种。

关键词: 蒟蒻薯属, DNA条形码, ITS, matK, rbcL, trnH-psbA

Abstract:

Tacca (Dioscoreaceae) is a small genus which is taxonomically difficult because of a lack of obvious morphologically differences among species. DNA barcoding is a new method of rapid species identification and discovery using short, standardized genes or DNA regions. In this study, four candidate DNA barcoding regions that three (rbcL, matK, trnHpsbA) from chloroplast genome and one (ITS) from the nuclear genome, were evaluated among 36 accessions representing 6 species of Tacca. The capability of those regions were evaluated by intra and interspecific divergence and barcode gap analyses, and the identification efficiency was assessed using Treebased and BBA methods. The results indicated that both ITS and the core barcode rbcL+matK proposed by CBOL exhibited the highest resolution as a single and combined data respectively. Based on overall performance: matK+rbcL can be considered as a potential barcode for identifying the species of Tacca, ITS can be used as a supplementary barcode. DNA barcoding revealed two distinct lineages of T.integrifolia distributed allopatrically in Tibet and Malaysia. And these two lineages with morphological variations may potentially represent new species.

Key words: Tacca, DNA barcoding, ITS, matK, rbcL, trnH-psbA

中图分类号:

Q 75，Q 949

赵月梅1、2, 张玲1. 蒟蒻薯属 (薯蓣科) 植物DNA条形码研究[J]. Plant Diversity, 2011, 33(6): 674-682.

DIAO Yue-Mei-, ZHANG Ling. Using DNA Barcoding in Genus Tacca (Dioscoreaceae)[J]. Plant Diversity, 2011, 33(6): 674-682.

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