MS1516是拟南芥四分体形成和小孢子发育的必需基因

doi:10.3724/SP.J.1143.2011.11029

摘要/Abstract

摘要：

以前报道了雄性育性下降突变体ms1516，而且图位克隆的方法已将突变基因MS1516定位到拟南芥基因组第3条染色体上28kb的区间内。本文通过进一步的生物信息学分析，发现该定位区间内有一个与减数分裂有关的基因AtATM，而且等位实验结果表明ms1516和atm3是等位突变体。细胞学分析结果表明，ms1516突变体在花药发育过程中产生多个不均等的小孢子，而且大多数的小孢子不能发育成成熟的花粉。DAPI染色的结果显示小孢子母细胞减数分裂过程中，染色体不能正常分离，对成熟花粉的扫描电镜观察结果发现突变体多数花粉形态异常。以上结果说明MS1516基因在小孢子形成和发育过程中具有重要作用。

关键词: 拟南芥, 雄性不育, 四分体, 减数分裂, MS1516基因

Abstract:

Anthers in flowers produce via meiosis haploid microspores, which mature to become pollen. However, pollen development at the molecular level is still not very clear. For the purpose of gaining understanding of the molecular control of pollen development, an Arabidopsis partial malesterility mutant ms1516 was reported previously, and the mutant gene MS1516 was mapped to a region of 28kb on chromosome 3 by a mapbased cloning approach. Here, we report that further bioinformatics analysis revealed that there was a meiosis related gene AtATM in this region. Allelism tests indicated that MS1516 and AtATM belonged to the same locus. Cell biology analysis showed that ms1516 mutant produced multiple uneven microspores during anther development, and most of which aborted at late stages. DAPI staining indicated that chromosome segregation was abnormal in ms1516 mutant meiocytes. Further scanning electron microscopy examination revealed abnormal pollen morphology formation in ms1516 mutant. Collectively these results demonstrated that MS1516 played an important role in tetrad formation and microspore development.

Key words: Arabidopsis thaliana, Male sterile, Tetrad, Meiosis, MS1516

中图分类号:

Q 75

赵先玲, 常海双, 杨仲南, 张森. MS1516是拟南芥四分体形成和小孢子发育的必需基因[J]. Plant Diversity, 2011, 33(4): 383-388.

ZHAO Xian-Ling, CHANG Hai-Shuang, YANG Zhong-Nan, ZHANG Sen. MS1516 is Essential for Tetrad Formation and Microspore Development in Arabidopsis thaliana[J]. Plant Diversity, 2011, 33(4): 383-388.

参考文献

Alexander MP, 1969. Differential staining of aborted and nonaborted pollen［J］. Stain Technology, 44: 117—122
Bai XF, Peisrson BN, Dong FG et al., 1999. Isolation and characterization of SYN1, a RAD210 like gene, is essential for meiosis in Arabidopsis［J］. Plant Cell, 11: 417—430
Dickinson HG, 1987. The physiology and biochemistry of meiosis in the anther［J］. International Review of Cytology, 107: 79—109
Dickinson HG, 1994. The regulation of alternation of generation in flowering plants［J］. Biological Reviews, 69: 419—422
Feng DD (冯丹丹), Wu YT (吴玉婷), Zhang F (张帆) et al., 2008. Mapping of an Arabidopsis gene involved in microspore development［J］. Acta Botanica Yunnanica (云南植物研究), 30 (4): 471—476
Garcia V, Salanoubat M, Choisne N et al., 2000. An ATM homologue from Arabidopsis thaliana: complete genomic organisation and expression analysis［J］. Nucleic Acid Research, 28: 1692—1699
Garcia V, Bruchet H, Camescasse D et al., 2003. AtATM Is essential for meiosis and the somatic response to DNA damage in plants［J］. Plant Cell, 15: 119—132
Glover J, Grelon M, Craig S et al., 1998. Cloning and characterization of MS5 from Arabidopsis: a gene critical in male meiosis［J］. Plant Journal, 15: 345—356
Guan YF, Huang XY, Zhu J et al., 2008. RUPTURED POLLEN GRAIN1, a member of the MtN3/saliva gene family, is crucial for exine pattern formation and cell integrity of microspores in Arabidopsis［J］. Plant Physiology, 147: 852—863
Goldberg RB, Beals TP, Sanders PM, 1993. Anther development: basic principles and practical application［J］. The Plant Cell, 5: 1217—1229
He C, Mascarenhas JP, 1998. MEI1, an Arabidopsis gene required for male meiosis: isolation and characterization［J］. Sexual Plant Reproduction, 11: 199—207
HeslopHarrison J, 1971. Wall pattern formation in angiosperm microsporogenesis［J］. Symposia of the Society for Experimental Biology, 25: 277—300
Jiang H, Wang F, Wu Y et al., 2009. MULTIPOLAR SPINDLE 1 (MPS1), a novel coiledcoil protein of Arabidopsis thaliana, is required for meiotic spindle organization［J］. Plant Journal, 59: 1001—1010
McCormick S, 1993. Male gametophyte development［J］. Plant Cell, 5: 1265—1275
Owen HA, Makaroff CA, 1995. Ultrastructure of microsporogenesis and microgametogenesis in Arabidopsis thaliana (L.) Heynh. Ecotype Wassilewskija (Brassicaceae)［J］. Protoplasma, 185: 7—21
PaxsonSowders DM, Owen HA, Makaroff CA, 1997. A comparative ultrastructural analysis of exine pattern development in wildtype Arabidopsis and a mutant defective in pattern formation［J］. Protoplasma, 198: 53—65
Reddy TV, Kaur J, Agashe B et al., 2003. The DUET gene is necessary for chromosome organization and progression during male meiosis in Arabidopsis and encodes a PHD finger protein［J］. Development, 130: 5975—5987
Ross KJ, Fransz P, Jones GH, 1996. A light microscopic atlas of meiosis in Arabidopsis thaliana［J］. Chromosome Research, 4: 507—516
Sanders PM, Bui AQ, Weterings K et al., 1999. Anther developmental defects in Arabidopsis thaliana malesterile mutants［J］. Sexual Plant Reproduction, 11(6): 297—322
Sheldon JM, Dickinson HG, 1983. Determination of patterning in the pollen wall of Lilium henryi［J］. Journal of Cell Science, 63: 191—208
Spielman M, Preuss D, Li FL et al., 1997. TETRASPORE is required for male meiotic cytokinesis in Arabidopsis thaliana［J］. Development, 124: 2645—2657
Yang M, Hu Y, Lodhi M et al., 1999. The Arabidopsis SKP1LIKE gene is essential for male meiosis and may control homologue separation［J］. Proceedings of the National Academy of Sciences of the United States of America, 96: 11416—11421
Yang X, Makaroff AC, Ma H, 2003. The Arabidopsis MALE MEIOCYTE DEATH1 gene encodes a PHDfinger protein that is required for male meiosis［J］. Plant Cell, 15: 1281—1295
Yang XH, Timofejeva L, Ma H et al., 2006. The Arabidopsis SKP1 homolog ASK1 controls meiotic chromosome remodeling and release of chromatin from the nuclear membrane and nucleolus［J］. Journal of Cell Science, 119: 3754—3763
Zhang ZB, Zhu J, Gao JF et al., 2007. Transcription factor AtMYB103 is required for anther development by regulating tapetum development, callose dissolution and exine formation in Arabidopsis［J］. Plant Journal, 52(3): 528—538

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