关键词: 箭毒木, 样品制备, 双向电泳

Abstract: We have established the extraction method of the total proteins of Antiaris toxicaria seed and the appropriate conditions for the two-dimensional eiectrophoresis (2-DE) of Antiaris toxicaria seed proteome . Through integrating and optimizing various conditions, we also established the 2-DE system including the sample preparation method with Tris-HCL based on TCA-actone , and the first-dimensional electrophoresis with immobilized pH gradients isoelectric focusing and the second dimensional electrophoresis with vertical electrophoresis of SDS-PAGE. We have gained a good 2-DE map in the analysis of sample preparation、sample resolution、isoelectric focusing ( IEF) 、SDS-PAGE and gel staining. In order to explore the extraction method of proteome and technique of 2-DE , we compared the extraction method between the TCA- actone and the Tris-HCl , and improved the key steps of 2-DE . The Tris-HCl extraction was the most appropriate for Antiaris toxicaria seed proteome analysis because of the highest resolution and more informative 2-DE map . Those were the solid basis for the later research of studying on the discrepancy expression proteomics of Antiaris toxicaria Seed .

Key words: Antiaris toxicaria