一氧化氮对小麦叶片镍毒害的缓解作用

Plant Diversity ›› 2007, Vol. 29 ›› Issue (01): 115-121.

一氧化氮对小麦叶片镍毒害的缓解作用

王松华, 周正义, 何庆元, 王晓鹏, 宋李红, 陆晓明

安徽科技学院生命科学学院

收稿日期:2006-03-10 出版日期:2007-02-25 发布日期:2007-02-25
通讯作者: 王松华

Nitric Oxide Alleviates the Nickel Toxicity in Wheat Seedlings

ANG Song-Hua , ZHOU Zheng-Yi , HE Qing-Yuan, Wang Xiao-Peng, SONG Li-Hong , LU Xiao-Ming

Life Science College, Anhui Science and Technology University

Received:2006-03-10 Online:2007-02-25 Published:2007-02-25
Contact: WANG Song-Hua

摘要/Abstract

摘要： 采用溶液培养法研究了重金属镍(Ni ) 对扬麦158 ( Triticum aestivum L) 幼苗生长的影响及外源一氧化氮(NO) 对Ni 毒害的缓解作用。结果表明, 100μmol/L Ni 处理显著抑制小麦幼苗生长, 导致叶绿素含量下降, 丙二醛(MDA) 含量显著升高, 且叶片过氧化物酶(POD)、超氧化物歧化酶(SOD) 和谷胱甘肽转硫酶(GST) 等抗氧化酶活性升高。400μmol/L 硝谱钠(SNP, NO 供体) 预处理2 d , 能够明显减轻Ni 毒害, 使叶绿素和MDA 含量基本恢复至对照水平。NO 可能是通过提高APX 和GR 等抗氧化酶的活性及谷胱甘肽含量而增强植株抗氧化能力, 显著减轻由Ni 导致的叶片Ca 和Fe 含量下降而增强小麦幼苗抵御Ni 毒害的能力。

关键词: 小麦, 镍, 一氧化氮, 抗氧化酶FONT

Abstract: Effects of nickel on the growth of wheat seedlings cultivated in solution and the alleviation of nitric oxide (NO)on the nickel toxicity were explored. Treatment of nickel at 100μmol/L caused dramatically reduction in seedling growth, decrease in chlorophyll levels and increase in MDA levels, and the increase in the activities of anti-oxidant enzymes, superoxide dismutase (SOD), guaiacol peroxidase (POD), and glutathione S- transferase (GST) in leaves. However, pretreatment of sodium nitroprusside (SNP), a NO donor , at 400μmol/L for 2 days alleviates significantly the nickel-induced symptom. The levels of chlorophyll and MDA in NO-pretreated seedlings were restored to the levels in the control. It is postulated that NO enhances the capacity of antioxidation in seedlings by increasing the activity of ascorbate peroxidase
(APX) and glutathionein reductase (GR), and the levels of glutathione, and mitigates reduction of contents of calcium and iron induced by nickel.

Key words: Wheat ( Triticum aestivum)

中图分类号:

Q 945

王松华, 周正义, 何庆元, 王晓鹏, 宋李红, 陆晓明. 一氧化氮对小麦叶片镍毒害的缓解作用[J]. Plant Diversity, 2007, 29(01): 115-121.

ANG Song-Hua , ZHOU Zheng-Yi , HE Qing-Yuan, Wang Xiao-Peng, SONG Li-Hong , LU Xiao-Ming. Nitric Oxide Alleviates the Nickel Toxicity in Wheat Seedlings[J]. Plant Diversity, 2007, 29(01): 115-121.

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