Eight new α-pyrone and γ-butenolide derivatives from the plant endophytic fungus Diaporthe sp. CCY4

doi:10.1007/s13659-025-00580-1

Natural Products and Bioprospecting ›› 2026, Vol. 16 ›› Issue (2): 29-29.DOI: 10.1007/s13659-025-00580-1

• ORIGINAL ARTICLES • Previous Articles Next Articles

Eight new α-pyrone and γ-butenolide derivatives from the plant endophytic fungus Diaporthe sp. CCY4

Jie-Chun Zeng, Xu-Ping Zhang, Lu Gao, Qian-Qian Yin, Wei-Guang Wang

Key Laboratory of Chemistry in Ethnic Medicinal Resources of Ministry of Education, Yunnan Minzu University, Kunming 650031, Yunnan, People's Republic of China

Received:2025-08-14 Online:2026-04-22 Published:2026-04-22
Contact: Qian-Qian Yin,Email:yinqianpharm@163.com;Wei-Guang Wang,Email:wwg@live.cn
Supported by:
This work was supported by grants from the National Natural Science Foundation of China (Project Nos 22377105 and 82160670), grants from the Natural Science Foundation of Yunnan Province (No. 202401AV070003), Yunnan Provincial Department of Education Science Research Fund Project (No. 2025J0492), and Yunnan Provincial Science and Technology Talent and Platform Program (202505AF350066).

Eight new α-pyrone and γ-butenolide derivatives from the plant endophytic fungus Diaporthe sp. CCY4

Jie-Chun Zeng, Xu-Ping Zhang, Lu Gao, Qian-Qian Yin, Wei-Guang Wang

Key Laboratory of Chemistry in Ethnic Medicinal Resources of Ministry of Education, Yunnan Minzu University, Kunming 650031, Yunnan, People's Republic of China

通讯作者: Qian-Qian Yin,Email:yinqianpharm@163.com;Wei-Guang Wang,Email:wwg@live.cn
基金资助:
This work was supported by grants from the National Natural Science Foundation of China (Project Nos 22377105 and 82160670), grants from the Natural Science Foundation of Yunnan Province (No. 202401AV070003), Yunnan Provincial Department of Education Science Research Fund Project (No. 2025J0492), and Yunnan Provincial Science and Technology Talent and Platform Program (202505AF350066).

Abstract

Abstract: Five new α-pyrones, diaporpyrones G-K (1-5) and three new γ-butenolide derivatives, porbutenolides A-C (6-8), along with seven known compounds (9-15), were isolated from the culture extract of the endophytic fungus Diaporthe sp. CCY4. Their structures were elucidated by comprehensive spectroscopic analysis, including 1D/2D NMR and HRESIMS data. The absolute configurations of 7 and 8 were assigned using electronic circular dichroism (ECD) calculations. All compounds were evaluated for inhibitory activity against ubiquitin-specific peptidase 4 (USP4). Compounds 2, 5, 9, and 13 exhibited significant anti-ubiquitination effects at 40 μM, with 13 showing the most potent inhibition (IC₅₀ = 20.85 μM).

Key words: α-pyrone, γ-butenolide, Diaporthe sp., USP4, Polyketides, Deubiquitinating enzymes

摘要： Five new α-pyrones, diaporpyrones G-K (1-5) and three new γ-butenolide derivatives, porbutenolides A-C (6-8), along with seven known compounds (9-15), were isolated from the culture extract of the endophytic fungus Diaporthe sp. CCY4. Their structures were elucidated by comprehensive spectroscopic analysis, including 1D/2D NMR and HRESIMS data. The absolute configurations of 7 and 8 were assigned using electronic circular dichroism (ECD) calculations. All compounds were evaluated for inhibitory activity against ubiquitin-specific peptidase 4 (USP4). Compounds 2, 5, 9, and 13 exhibited significant anti-ubiquitination effects at 40 μM, with 13 showing the most potent inhibition (IC₅₀ = 20.85 μM).

关键词: α-pyrone, γ-butenolide, Diaporthe sp., USP4, Polyketides, Deubiquitinating enzymes

Jie-Chun Zeng, Xu-Ping Zhang, Lu Gao, Qian-Qian Yin, Wei-Guang Wang. Eight new α-pyrone and γ-butenolide derivatives from the plant endophytic fungus Diaporthe sp. CCY4[J]. Natural Products and Bioprospecting, 2026, 16(2): 29-29.

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Eight new α-pyrone and γ-butenolide derivatives from the plant endophytic fungus Diaporthe sp. CCY4

Eight new α-pyrone and γ-butenolide derivatives from the plant endophytic fungus Diaporthe sp. CCY4

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