Integrative Biology Journals

Natural Products and Bioprospecting ›› 2012, Vol. 2 ›› Issue (6): 240-244.DOI: 10.1007/s13659-012-0058-4

• Regular Article • Previous Articles     Next Articles

Six novel steroids from culture of basidiomycete Polyporus ellisii

Shuang WANGa,b, Ling ZHANGa, Liang-Yan LIUa,b, Ze-Jun DONGa, Zheng-Hui LIa, Ji-Kai LIUa   

  1. a State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    b University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2012-07-17 Revised:2012-10-08 Online:2018-02-11 Published:2012-12-24
  • Supported by:
    This work was financially supported by National Basic Research Program of China(973 Program, 2009CB522300), the National Natural Science Foundation of China(30830113, U1132607).

Six novel steroids from culture of basidiomycete Polyporus ellisii

Shuang WANGa,b, Ling ZHANGa, Liang-Yan LIUa,b, Ze-Jun DONGa, Zheng-Hui LIa, Ji-Kai LIUa   

  1. a State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
    b University of Chinese Academy of Sciences, Beijing 100049, China
  • 通讯作者: Ji-Kai LIU,E-mail:jkliu@mail.kib.ac.cn
  • 基金资助:
    This work was financially supported by National Basic Research Program of China(973 Program, 2009CB522300), the National Natural Science Foundation of China(30830113, U1132607).

Abstract: Investigation of the culture of basidiomycete Polyporus ellisii led to the isolation of a novel compound 3β, 9α, 15α-trihydroxy-(22E, 24R)-10(5→4)-abeo-ergosta-6, 8(14), 22-trien-5-one(1) with a new 5/7/6/5 ring system of ergosterol skeleton. In addition, five new steroids, 5β, 6β-epoxy-3β, 7α, 9α-trihydroxy-(22E, 24R)-ergosta-8(14), 22-dien-15-one(2), 5β, 6β-epoxy-3β, 7α-dihydroxy-(22E, 24R)-ergosta-8(14), 22-dien-15-one(3), 5α, 6α-epoxy-3β, 9α, 15α-trihydroxy-(22E, 24R)-ergosta-8(14), 22-dien-7-one(4), 15α-acetoxy-(22E, 24R)-ergosta-4, 6, 8(14), 22-tetraen-3-one(5), 15β-methoxy-(22E, 24R)-ergosta-4, 6, 8(14), 22-tetraen-3-one(6), along with four known ergosterols(7-10), were obtained. All structures were elucidated based on 1D and 2D NMR spectral data. New compounds were evaluated for cytotoxicity against five human cancer cell lines, only compound 4 was found to exhibit a favorable cytotoxicity profile toward all tested tumor cell lines.

Key words: ergosterol, Polyporus ellisii, basidiomycete, cytotoxicity

摘要: Investigation of the culture of basidiomycete Polyporus ellisii led to the isolation of a novel compound 3β, 9α, 15α-trihydroxy-(22E, 24R)-10(5→4)-abeo-ergosta-6, 8(14), 22-trien-5-one(1) with a new 5/7/6/5 ring system of ergosterol skeleton. In addition, five new steroids, 5β, 6β-epoxy-3β, 7α, 9α-trihydroxy-(22E, 24R)-ergosta-8(14), 22-dien-15-one(2), 5β, 6β-epoxy-3β, 7α-dihydroxy-(22E, 24R)-ergosta-8(14), 22-dien-15-one(3), 5α, 6α-epoxy-3β, 9α, 15α-trihydroxy-(22E, 24R)-ergosta-8(14), 22-dien-7-one(4), 15α-acetoxy-(22E, 24R)-ergosta-4, 6, 8(14), 22-tetraen-3-one(5), 15β-methoxy-(22E, 24R)-ergosta-4, 6, 8(14), 22-tetraen-3-one(6), along with four known ergosterols(7-10), were obtained. All structures were elucidated based on 1D and 2D NMR spectral data. New compounds were evaluated for cytotoxicity against five human cancer cell lines, only compound 4 was found to exhibit a favorable cytotoxicity profile toward all tested tumor cell lines.

关键词: ergosterol, Polyporus ellisii, basidiomycete, cytotoxicity