Study on the Structural Effect of Maltoligosaccharides on Cytochrome c Complexes Stabilities by Native Mass Spectrometry

doi:10.1007/s13659-017-0150-x

Natural Products and Bioprospecting ›› 2018, Vol. 8 ›› Issue (1): 57-61.DOI: 10.1007/s13659-017-0150-x

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Study on the Structural Effect of Maltoligosaccharides on Cytochrome c Complexes Stabilities by Native Mass Spectrometry

Quan Chi, Ying-Zhi Liu, Xian Wang

Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, Hubei 430074, People's Republic of China

Received:2017-11-13 Revised:2017-12-13 Online:2018-02-27 Published:2018-02-24
Contact: Xian Wang

Study on the Structural Effect of Maltoligosaccharides on Cytochrome c Complexes Stabilities by Native Mass Spectrometry

Quan Chi, Ying-Zhi Liu, Xian Wang

Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, Hubei 430074, People's Republic of China

基金资助:
This research was supported by the National Natural Science Foundation of China (Grant No. 21675176), the Natural Science Foundation of Hubei Province (Grant No. 2014CFA025), and the Preferred Research Foundation for the Returned Overseas Scholars from Ministry of Human Resources and Social Security of the People's Republic of China (Grant No. BZY14036) for financial supports.

Abstract

Abstract: Noncovalent interactions between ligands and targeting proteins are essential for understanding molecular mechanisms of proteins. In this work, we investigated the interaction of Cytochrome c (Cyt c) with maltoligosaccharides, namely maltose (Mal Ⅱ), maltotriose (Mal Ⅲ), maltotetraose (Mal IV), maltopentaose (Mal V), maltohexaose (Mal VI) and maltoheptaose (Mal VⅡ). Using electrospray ionization mass spetrometry (ESI-MS) assay, the 1:1 and 1:2 complexes formed by Cyt c with maltoligosaccharide ligand were observed. The corresponding association constants were calculated according to the deconvoluted spectra. The order of the relative binding affinities of the selected oligosaccharides with Cyt c were as Mal Ⅲ > Mal IV > Mal Ⅱ > Mal V > Mal VI > Mal VⅡ. The results indicated that the stability of noncovalent protein complexes was intimately correlated to the molecular structure of bound ligand. The relevant functional groups that could form H-bonds, electrostatic or hydrophobic forces with protein's amino residues played an important role for the stability of protein complexes. In addition, the steric structure of ligand was also critical for an appropriate interaction with the binding pocket of proteins.

Key words: Electrospray ionization mass spectrometry, Maltoligosaccharides, Cytochrome c complexes, Structure-binding relationship

摘要： Noncovalent interactions between ligands and targeting proteins are essential for understanding molecular mechanisms of proteins. In this work, we investigated the interaction of Cytochrome c (Cyt c) with maltoligosaccharides, namely maltose (Mal Ⅱ), maltotriose (Mal Ⅲ), maltotetraose (Mal IV), maltopentaose (Mal V), maltohexaose (Mal VI) and maltoheptaose (Mal VⅡ). Using electrospray ionization mass spetrometry (ESI-MS) assay, the 1:1 and 1:2 complexes formed by Cyt c with maltoligosaccharide ligand were observed. The corresponding association constants were calculated according to the deconvoluted spectra. The order of the relative binding affinities of the selected oligosaccharides with Cyt c were as Mal Ⅲ > Mal IV > Mal Ⅱ > Mal V > Mal VI > Mal VⅡ. The results indicated that the stability of noncovalent protein complexes was intimately correlated to the molecular structure of bound ligand. The relevant functional groups that could form H-bonds, electrostatic or hydrophobic forces with protein's amino residues played an important role for the stability of protein complexes. In addition, the steric structure of ligand was also critical for an appropriate interaction with the binding pocket of proteins.

关键词: Electrospray ionization mass spectrometry, Maltoligosaccharides, Cytochrome c complexes, Structure-binding relationship

Quan Chi, Ying-Zhi Liu, Xian Wang. Study on the Structural Effect of Maltoligosaccharides on Cytochrome c Complexes Stabilities by Native Mass Spectrometry[J]. Natural Products and Bioprospecting, 2018, 8(1): 57-61.

Quan Chi, Ying-Zhi Liu, Xian Wang. Study on the Structural Effect of Maltoligosaccharides on Cytochrome c Complexes Stabilities by Native Mass Spectrometry[J]. 应用天然产物, 2018, 8(1): 57-61.

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Study on the Structural Effect of Maltoligosaccharides on Cytochrome c Complexes Stabilities by Native Mass Spectrometry

Study on the Structural Effect of Maltoligosaccharides on Cytochrome c Complexes Stabilities by Native Mass Spectrometry

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