Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

doi:10.1007/s13659-023-00384-1

Natural Products and Bioprospecting ›› 2023, Vol. 13 ›› Issue (3): 18-18.DOI: 10.1007/s13659-023-00384-1

• 2023-6-25 • Previous Articles Next Articles

Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

Luan Wen^1,2, Zhou-Wei Wu^1,2, Li-Wu Lin^1,2, Abdulbaset Al-Romaima^1,2, Xing-Rong Peng^1,2, Ming-Hua Qiu^1,2

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, People’s Republic of China;
2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

Received:2023-05-05 Online:2023-07-18 Published:2023-06-24
Contact: Ming-Hua Qiu,E-mail:mhchiu@mail.kib.ac.cn
Supported by:
The research work was financially supported by the National Natural Science Foundation of China (No. 31872675), as well as the Cooperation Project with DR PLANT Company (2023).

Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

Luan Wen^1,2, Zhou-Wei Wu^1,2, Li-Wu Lin^1,2, Abdulbaset Al-Romaima^1,2, Xing-Rong Peng^1,2, Ming-Hua Qiu^1,2

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, People’s Republic of China;
2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

通讯作者: Ming-Hua Qiu,E-mail:mhchiu@mail.kib.ac.cn
基金资助:
The research work was financially supported by the National Natural Science Foundation of China (No. 31872675), as well as the Cooperation Project with DR PLANT Company (2023).

Abstract

Abstract: Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp. Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses. Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6. Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase. MCPb (Mw=10.1 kDa) and MCPc (Mw=5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.

Key words: Lepidium meyenii, Polysaccharide, Molecular weight, Structural characterization, α-Glucosidase inhibition

摘要： Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp. Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses. Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6. Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase. MCPb (Mw=10.1 kDa) and MCPc (Mw=5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.

关键词: Lepidium meyenii, Polysaccharide, Molecular weight, Structural characterization, α-Glucosidase inhibition

Luan Wen, Zhou-Wei Wu, Li-Wu Lin, Abdulbaset Al-Romaima, Xing-Rong Peng, Ming-Hua Qiu. Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights[J]. Natural Products and Bioprospecting, 2023, 13(3): 18-18.

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Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

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