Brown Algae(Phaeophyceae) from the Coast of Madagascar:preliminary Bioactivity Studies and Isolation of Natural Products

doi:10.1007/s13659-015-0068-0

Abstract

Abstract: Eight species of brown algae(Phaeophyceae) from the coast of Madagascar have been investigated for their chemical constituents. Fucosterol(3) was obtained as the most abundant compound. The brown alga Sargassum ilicifolium was the source for the first isolation of the terpenoid C₂₇-alcohol 1, 1', 2-trinorsqualenol(1) from marine sources. From S. incisifolium we isolated the highly unsaturated glycolipid 1-O-palmitoyl-2-O-stearidonoyl-3-O-β-D-galactopyranosylglycerol(4) and we report the first full assignment of its ¹H and ¹³C NMR data. Apo-9'-fucoxanthinone(8) along with 24-ketocholesterol(5),(22E)-3β-hydroxycholesta-5, 22-dien-24-one(6), and saringosterol(7) were obtained from Turbinaria ornata. The crude extracts of all eight species of brown algae exhibited a pronounced antimicrobial activity against the Gram-positive bacteria Bacillus cereus, Staphylococcus aureus, and Streptococcus pneumoniae.

Key words: Brown algae, Steroids, Terpenoids, NMR spectroscopy, Antimicrobial activity

摘要： Eight species of brown algae(Phaeophyceae) from the coast of Madagascar have been investigated for their chemical constituents. Fucosterol(3) was obtained as the most abundant compound. The brown alga Sargassum ilicifolium was the source for the first isolation of the terpenoid C₂₇-alcohol 1, 1', 2-trinorsqualenol(1) from marine sources. From S. incisifolium we isolated the highly unsaturated glycolipid 1-O-palmitoyl-2-O-stearidonoyl-3-O-β-D-galactopyranosylglycerol(4) and we report the first full assignment of its ¹H and ¹³C NMR data. Apo-9'-fucoxanthinone(8) along with 24-ketocholesterol(5),(22E)-3β-hydroxycholesta-5, 22-dien-24-one(6), and saringosterol(7) were obtained from Turbinaria ornata. The crude extracts of all eight species of brown algae exhibited a pronounced antimicrobial activity against the Gram-positive bacteria Bacillus cereus, Staphylococcus aureus, and Streptococcus pneumoniae.

关键词: Brown algae, Steroids, Terpenoids, NMR spectroscopy, Antimicrobial activity

Marie Pascaline Rahelivao, Margit Gruner, Hanta Andriamanantoanina, Ingmar Bauer, Hans-Joachim Knölker. Brown Algae(Phaeophyceae) from the Coast of Madagascar:preliminary Bioactivity Studies and Isolation of Natural Products[J]. Natural Products and Bioprospecting, 2015, 5(5): 223-235.

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