Abstract: A quantitative ¹H NMR method (qHNMR) was used to measure the trigonelline content in the leaves of six species of the Annona genus. The methodology employed compared the intensities of the signals at δ 9.14 (H-2) and δ 0.00, the internal standard TSP-d₄. This measuring method was able to establish the concentration of trigonelline in the range from 0.67 to 10.04 mg·g^-1 depending on the investigated extract.

Key words: trigonelline, Annona, quantitative ¹H NMR

摘要： A quantitative ¹H NMR method (qHNMR) was used to measure the trigonelline content in the leaves of six species of the Annona genus. The methodology employed compared the intensities of the signals at δ 9.14 (H-2) and δ 0.00, the internal standard TSP-d₄. This measuring method was able to establish the concentration of trigonelline in the range from 0.67 to 10.04 mg·g^-1 depending on the investigated extract.

关键词: trigonelline, Annona, quantitative ¹H NMR