Pyridone alkaloids from an Antarctic endolichenic Tolypocladium sp.

doi:10.1007/s13659-026-00607-1

Natural Products and Bioprospecting ›› 2026, Vol. 16 ›› Issue (4): 46-46.DOI: 10.1007/s13659-026-00607-1

• ORIGINAL ARTICLES • Next Articles

Pyridone alkaloids from an Antarctic endolichenic Tolypocladium sp.

Shasha Li¹, Ting Yu¹, Jianju Feng², Yue Shang¹, Tao Zhang², Shuzhen Chen¹, Liyan Yu², Maoluo Gan¹

1. Beijing Key Laboratory of Technology and Application for Anti-Infective New Drugs Research and Development, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;
2. China Pharmaceutical Culture Collection, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China

Received:2025-11-17 Accepted:2026-02-09 Online:2026-08-29 Published:2026-08-22
Contact: Maoluo Gan,E-mail:ganml@imb.pumc.edu.cn
Supported by:
This work was financially supported by CAMS Innovation Fund for Medical Sciences (CIFMS, 2021-I2M-1-055 and 2024-I2M-TS-016), National Natural Science Foundation of China (Grant no. 82273830), the National Key R&D Program of China (Grant no. 2024YFC2309604), and the National Microbial Resources Center (No. NMRC-2025-3).

Pyridone alkaloids from an Antarctic endolichenic Tolypocladium sp.

Shasha Li¹, Ting Yu¹, Jianju Feng², Yue Shang¹, Tao Zhang², Shuzhen Chen¹, Liyan Yu², Maoluo Gan¹

1. Beijing Key Laboratory of Technology and Application for Anti-Infective New Drugs Research and Development, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;
2. China Pharmaceutical Culture Collection, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China

通讯作者: Maoluo Gan,E-mail:ganml@imb.pumc.edu.cn
基金资助:
This work was financially supported by CAMS Innovation Fund for Medical Sciences (CIFMS, 2021-I2M-1-055 and 2024-I2M-TS-016), National Natural Science Foundation of China (Grant no. 82273830), the National Key R&D Program of China (Grant no. 2024YFC2309604), and the National Microbial Resources Center (No. NMRC-2025-3).

Abstract

Abstract: Three new pyridone alkaloids, tolypyrone A (1), tolypyrosides A (2) and B (3), and a new tetramic acid 2′-epitolypoalbin (4), along with ten known compounds, were obtained from Tolypocladium sp. CPCC 401485 isolated from an unidentified Antarctic lichen specimen. Structural elucidation of new compounds was achieved by analyses of HRESIMS and NMR spectroscopic data combined with X-ray crystallography, advanced Marfey's method, and ECD calculation. Tolypyrone A (1) was the first 4-pyridone alkaloid with an unusual octahydro-1H-isochromeno[3,4-b]pyridin-4-one triheterocyclic ring system. Compounds 1, 4 and 6 exhibited antimicrobial activities with the minimum inhibition concentration of 16-64 μg/mL.

Key words: Pyridone alkaloids, Tetramic acid, Antarctic microbes, Endolichenic fungus, Tolypocladium sp., Antimicrobial activities

摘要： Three new pyridone alkaloids, tolypyrone A (1), tolypyrosides A (2) and B (3), and a new tetramic acid 2′-epitolypoalbin (4), along with ten known compounds, were obtained from Tolypocladium sp. CPCC 401485 isolated from an unidentified Antarctic lichen specimen. Structural elucidation of new compounds was achieved by analyses of HRESIMS and NMR spectroscopic data combined with X-ray crystallography, advanced Marfey's method, and ECD calculation. Tolypyrone A (1) was the first 4-pyridone alkaloid with an unusual octahydro-1H-isochromeno[3,4-b]pyridin-4-one triheterocyclic ring system. Compounds 1, 4 and 6 exhibited antimicrobial activities with the minimum inhibition concentration of 16-64 μg/mL.

关键词: Pyridone alkaloids, Tetramic acid, Antarctic microbes, Endolichenic fungus, Tolypocladium sp., Antimicrobial activities

Shasha Li, Ting Yu, Jianju Feng, Yue Shang, Tao Zhang, Shuzhen Chen, Liyan Yu, Maoluo Gan. Pyridone alkaloids from an Antarctic endolichenic Tolypocladium sp.[J]. Natural Products and Bioprospecting, 2026, 16(4): 46-46.

Shasha Li, Ting Yu, Jianju Feng, Yue Shang, Tao Zhang, Shuzhen Chen, Liyan Yu, Maoluo Gan. Pyridone alkaloids from an Antarctic endolichenic Tolypocladium sp.[J]. 应用天然产物, 2026, 16(4): 46-46.

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Pyridone alkaloids from an Antarctic endolichenic Tolypocladium sp.

Pyridone alkaloids from an Antarctic endolichenic Tolypocladium sp.

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