Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest

doi:10.1007/s13659-020-00240-6

Natural Products and Bioprospecting ›› 2020, Vol. 10 ›› Issue (3): 131-140.DOI: 10.1007/s13659-020-00240-6

• ORIGINAL ARTICLES • Previous Articles Next Articles

Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest

Rong-Fang Mu^1,4, Yan-Fen Niu³, Qian Wang^1,4, Hui-Min Zhou^1,4, Jing Hu¹, Wan-Ying Qin^1,4, Wen-Yong Xiong^1,2,4

1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People's Republic of China;
2 Key Laboratory of Medicinal Chemistry for Natural Resource, Yunnan University, Kunming 650500, Yunnan, People's Republic of China;
3 Biomedical Engineering Research Center, Kunming Medical University, Kunming 650500, Yunnan, People's Republic of China;
4 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China

Received:2020-03-17 Revised:2020-04-04 Online:2020-06-02 Published:2020-06-24
Contact: Wen-Yong Xiong, Jing Hu
Supported by:
Thanks Dr. Jing Hu, Yan-Ting Lu and Fang Wang for discussing the work. This work was supported by National Key R&D Program of China, (2017YFC1700906) and Yunnan Provincial Science and Technology Department, China (2017FA044 and 2013HA023).

Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest

Rong-Fang Mu^1,4, Yan-Fen Niu³, Qian Wang^1,4, Hui-Min Zhou^1,4, Jing Hu¹, Wan-Ying Qin^1,4, Wen-Yong Xiong^1,2,4

1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, People's Republic of China;
2 Key Laboratory of Medicinal Chemistry for Natural Resource, Yunnan University, Kunming 650500, Yunnan, People's Republic of China;
3 Biomedical Engineering Research Center, Kunming Medical University, Kunming 650500, Yunnan, People's Republic of China;
4 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China

通讯作者: Wen-Yong Xiong, Jing Hu
基金资助:
Thanks Dr. Jing Hu, Yan-Ting Lu and Fang Wang for discussing the work. This work was supported by National Key R&D Program of China, (2017YFC1700906) and Yunnan Provincial Science and Technology Department, China (2017FA044 and 2013HA023).

Abstract

Abstract: Eriocalyxin B, an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx, has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory. However, the function and mechanism of the compound in adipocyte differentiation is still unknown. Here we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets, triglycerides and the expressions of adipogenesis-related factors, including C/EBPβ, C/EBPα, PPARγ, and FABP4. Moreover, we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation, most likely by suppressing mRNAs and proteins of CDK1, CDK2, Cyclin A and Cyclin B1. Overall, we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.

Key words: Eriocalyxin B, Adipocyte differentiation, Cell cycle

摘要： Eriocalyxin B, an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx, has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory. However, the function and mechanism of the compound in adipocyte differentiation is still unknown. Here we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets, triglycerides and the expressions of adipogenesis-related factors, including C/EBPβ, C/EBPα, PPARγ, and FABP4. Moreover, we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation, most likely by suppressing mRNAs and proteins of CDK1, CDK2, Cyclin A and Cyclin B1. Overall, we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.

关键词: Eriocalyxin B, Adipocyte differentiation, Cell cycle

Rong-Fang Mu, Yan-Fen Niu, Qian Wang, Hui-Min Zhou, Jing Hu, Wan-Ying Qin, Wen-Yong Xiong. Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest[J]. Natural Products and Bioprospecting, 2020, 10(3): 131-140.

Rong-Fang Mu, Yan-Fen Niu, Qian Wang, Hui-Min Zhou, Jing Hu, Wan-Ying Qin, Wen-Yong Xiong. Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest[J]. 应用天然产物, 2020, 10(3): 131-140.

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Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest

Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest

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