Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii

doi:10.1007/s13659-014-0051-1

Natural Products and Bioprospecting ›› 2015, Vol. 5 ›› Issue (1): 47-53.DOI: 10.1007/s13659-014-0051-1

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Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii

Yang Wang^1,2, Ling Zhang¹, Gen-Tao Li¹, Zheng-Hui Li¹, Ze-Jun Dong¹, Yan Li¹, Ji-Kai Liu¹

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2014-11-13 Revised:2014-12-07 Online:2018-02-11 Published:2015-02-24
Supported by:
This work was supported by the National Natural Sciences Foundation of China(U1132607, 21302194), the National Science & Technology Pillar Program of China(2012BAD23B0305 and 2013BAI11B02).

Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii

Yang Wang^1,2, Ling Zhang¹, Gen-Tao Li¹, Zheng-Hui Li¹, Ze-Jun Dong¹, Yan Li¹, Ji-Kai Liu¹

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

通讯作者: Ji-Kai Liu,e-mail:jkliu@mail.kib.ac.cn
基金资助:
This work was supported by the National Natural Sciences Foundation of China(U1132607, 21302194), the National Science & Technology Pillar Program of China(2012BAD23B0305 and 2013BAI11B02).

Abstract

Abstract: Engleromyces goetzii is a traditional medicinal mushroom that is widely used to treat infection, inflammation and cancer in Tibet, Sichuan and Yunnan provinces of China. Two new trichothecenes, engleromycones A and B(1 and 2), one new cuparane-type sesquiterpenoid named infuscol F(11), eight known trichothecene analogs, sambucinol(3), 3-deoxysambucinol(4), trichothecolone(5), trichodermol(6), 8-deoxytrichothecin(7), trichothecin(8), trichothecinol B(9) and trichothecinol A(10), and one known cyclopentanoid sesquiterpene cyclonerodiol(12) were isolated from the cultures of E. goetzii. The new compounds were elucidated through spectroscopic analyses. The anticancer effects of trichothecenes 1-10 were examined in the HL-60, SMMC-7721, A549, MCF-7, and SW-480 human cancer cell lines using an MTT assay. Trichothecinol A(10) significantly inhibited the growth of MCF-7 cells, with an IC₅₀ value of 0.006 μM, which was comparable to the cytotoxic activity of the positive control, paclitaxel, indicating that trichothecinol A(10) represents a potential anticancer agent.

Key words: Engleromyces goetzii, Trichothecene, Engleromycone, Cuparane, Infuscol, Mycotoxin

摘要： Engleromyces goetzii is a traditional medicinal mushroom that is widely used to treat infection, inflammation and cancer in Tibet, Sichuan and Yunnan provinces of China. Two new trichothecenes, engleromycones A and B(1 and 2), one new cuparane-type sesquiterpenoid named infuscol F(11), eight known trichothecene analogs, sambucinol(3), 3-deoxysambucinol(4), trichothecolone(5), trichodermol(6), 8-deoxytrichothecin(7), trichothecin(8), trichothecinol B(9) and trichothecinol A(10), and one known cyclopentanoid sesquiterpene cyclonerodiol(12) were isolated from the cultures of E. goetzii. The new compounds were elucidated through spectroscopic analyses. The anticancer effects of trichothecenes 1-10 were examined in the HL-60, SMMC-7721, A549, MCF-7, and SW-480 human cancer cell lines using an MTT assay. Trichothecinol A(10) significantly inhibited the growth of MCF-7 cells, with an IC₅₀ value of 0.006 μM, which was comparable to the cytotoxic activity of the positive control, paclitaxel, indicating that trichothecinol A(10) represents a potential anticancer agent.

关键词: Engleromyces goetzii, Trichothecene, Engleromycone, Cuparane, Infuscol, Mycotoxin

Yang Wang, Ling Zhang, Gen-Tao Li, Zheng-Hui Li, Ze-Jun Dong, Yan Li, Ji-Kai Liu. Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii[J]. Natural Products and Bioprospecting, 2015, 5(1): 47-53.

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Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii

Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii

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