In vitro bone inducing effects of Lentinula edodes (shiitake) water extract on human osteoblastic cell cultures

doi:10.1007/s13659-013-0070-3

Natural Products and Bioprospecting ›› 2013, Vol. 3 ›› Issue (6): 282-287.DOI: 10.1007/s13659-013-0070-3

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In vitro bone inducing effects of Lentinula edodes (shiitake) water extract on human osteoblastic cell cultures

Ashraf SAIF^a, Kristian WENDE^b, Ulrike LINDEQUIST^b

a Al-Leith University College, Umm Al-Qura University, Makkah 311, Saudi Arabia;
b Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Ludwig-Jahn-Stra?e 17, D-17489 Greifswald, Germany

Received:2013-09-13 Revised:2013-11-16 Online:2018-02-11 Published:2013-12-24

In vitro bone inducing effects of Lentinula edodes (shiitake) water extract on human osteoblastic cell cultures

Ashraf SAIF^a, Kristian WENDE^b, Ulrike LINDEQUIST^b

a Al-Leith University College, Umm Al-Qura University, Makkah 311, Saudi Arabia;
b Institute of Pharmacy, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Ludwig-Jahn-Stra?e 17, D-17489 Greifswald, Germany

通讯作者: Ashraf SAIF,E-mail:aaa_saif@hotmail.com

Abstract

Abstract: The effect of Lentinula edodes water extract (LE) on two osteoblastic cell cultures (HOS 58 and Saos-2) was investigated to determine if this edible medicinal mushroom has osteoinductive properties. Activity of alkaline phosphatase and mineralization were used as indicators for the vitality and maturation of the bone cells. Cultivation of human osteosarcoma cells HOS 58 for five days in presence of a serial dilution of the aqueous extract of L. edodes (0.8 μg/mL-125 μg/mL) resulted in a significant elevation of alkaline phosphatase activity (ALP) of the cells in comparison to untreated cells. Saos-2 cells, incubated with LE (20 μg/mL) and β-glycerol phosphate (2 mM) for 21 days, displayed a 2 fold level of mineralization than cells cultured soley with the positive control, β-glycerophosphate. The obtained results clearly indicate the activity of LE as a bone inducing agent in vitro. Therefore, the shiitake mushroom (L. edodes) deserves attention as a supportive dietary treatment or nutraceutical in the case of diseases accompanied with bone disorder, such as osteoporosis, osteopenia, and late complication of diabetes.

Key words: medicinal mushrooms, Lentinula edodes, osteoporosis, alkaline phosphatase, bone mineralization, shiitake

摘要： The effect of Lentinula edodes water extract (LE) on two osteoblastic cell cultures (HOS 58 and Saos-2) was investigated to determine if this edible medicinal mushroom has osteoinductive properties. Activity of alkaline phosphatase and mineralization were used as indicators for the vitality and maturation of the bone cells. Cultivation of human osteosarcoma cells HOS 58 for five days in presence of a serial dilution of the aqueous extract of L. edodes (0.8 μg/mL-125 μg/mL) resulted in a significant elevation of alkaline phosphatase activity (ALP) of the cells in comparison to untreated cells. Saos-2 cells, incubated with LE (20 μg/mL) and β-glycerol phosphate (2 mM) for 21 days, displayed a 2 fold level of mineralization than cells cultured soley with the positive control, β-glycerophosphate. The obtained results clearly indicate the activity of LE as a bone inducing agent in vitro. Therefore, the shiitake mushroom (L. edodes) deserves attention as a supportive dietary treatment or nutraceutical in the case of diseases accompanied with bone disorder, such as osteoporosis, osteopenia, and late complication of diabetes.

关键词: medicinal mushrooms, Lentinula edodes, osteoporosis, alkaline phosphatase, bone mineralization, shiitake

Ashraf SAIF, Kristian WENDE, Ulrike LINDEQUIST. In vitro bone inducing effects of Lentinula edodes (shiitake) water extract on human osteoblastic cell cultures[J]. Natural Products and Bioprospecting, 2013, 3(6): 282-287.

Ashraf SAIF, Kristian WENDE, Ulrike LINDEQUIST. In vitro bone inducing effects of Lentinula edodes (shiitake) water extract on human osteoblastic cell cultures[J]. 应用天然产物, 2013, 3(6): 282-287.

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In vitro bone inducing effects of Lentinula edodes (shiitake) water extract on human osteoblastic cell cultures

In vitro bone inducing effects of Lentinula edodes (shiitake) water extract on human osteoblastic cell cultures

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