Antifungal alkaloids from Mahonia fortunei against pathogens of postharvest fruit

doi:10.1007/s13659-023-00374-3

应用天然产物 ›› 2023, Vol. 13 ›› Issue (2): 10-10.DOI: 10.1007/s13659-023-00374-3

• Original Article • 上一篇下一篇

Antifungal alkaloids from Mahonia fortunei against pathogens of postharvest fruit

Xiao-Na Wang¹, Zhao-Jie Wang¹, Yun Zhao², Huan Wang¹, Mei-Ling Xiang¹, Yang-Yang Liu¹, Li-Xing Zhao¹, Xiao-Dong Luo^1,2

1. Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of Chemical Science and Technology, Yunnan University, Kunming, 650500, People's Republic of China;
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming, Kunming, 650201, People's Republic of China

收稿日期:2022-12-09 出版日期:2023-04-24 发布日期:2023-05-18
通讯作者: Li-Xing Zhao,E-mail:lixingzhao@ynu.edu.cn;Xiao-Dong Luo,E-mail:xdluo@ynu.edu.cn
基金资助:
This work was supported by NSFC (32170405), the High-level Talent Promotion and Training Project of Kunming (2022SCP003), Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform (2022YKZY001), and Scientific and Technological Innovation Team of Yunnan Province (202105AE160006). We thank the Advanced Analysis and Measurement Center of Yunnan University for technical support.

Antifungal alkaloids from Mahonia fortunei against pathogens of postharvest fruit

Xiao-Na Wang¹, Zhao-Jie Wang¹, Yun Zhao², Huan Wang¹, Mei-Ling Xiang¹, Yang-Yang Liu¹, Li-Xing Zhao¹, Xiao-Dong Luo^1,2

1. Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of Chemical Science and Technology, Yunnan University, Kunming, 650500, People's Republic of China;
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming, Kunming, 650201, People's Republic of China

Received:2022-12-09 Online:2023-04-24 Published:2023-05-18
Contact: Li-Xing Zhao,E-mail:lixingzhao@ynu.edu.cn;Xiao-Dong Luo,E-mail:xdluo@ynu.edu.cn
Supported by:
This work was supported by NSFC (32170405), the High-level Talent Promotion and Training Project of Kunming (2022SCP003), Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform (2022YKZY001), and Scientific and Technological Innovation Team of Yunnan Province (202105AE160006). We thank the Advanced Analysis and Measurement Center of Yunnan University for technical support.

摘要/Abstract

摘要： Postharvest pathogens can affect a wide range of fresh fruit and vegetables, including grapes, resulting in significant profit loss. Isoquinoline alkaloids of Mahonia fortunei, a Chinese herbal medicine, have been used to treat infectious microbes, which might be effective against postharvest pathogens. The phytochemical and bioactive investigation of this plant led to the isolation of 18 alkaloids, of which 9 compounds inhibited the growth of Botrytis cinerea and 4 compounds against Penicillium italicum. The antifungal alkaloids could change the mycelium morphology, the total lipid content, and leak the cell contents of B. cinerea. Furthermore, the two most potent antifungal alkaloids, berberine (13) completely inhibited effect on gray mold of table grape at 512 mg L^-1, while jatrorrhizine (18) exhibited an inhibition rate > 90% on grape rot at the same concentration, with lower cytotoxicity and residue than chlorothalonil, which suggested that ingredients of M. fortunei might be a low-toxicity, low-residue, eco-friendly botanical fungicide against postharvest pathogens.

关键词: Mahonia fortunei, Chemical constituents, Botrytis cinerea, Penicillium italicum, Anti-postharvest pathogens

Abstract: Postharvest pathogens can affect a wide range of fresh fruit and vegetables, including grapes, resulting in significant profit loss. Isoquinoline alkaloids of Mahonia fortunei, a Chinese herbal medicine, have been used to treat infectious microbes, which might be effective against postharvest pathogens. The phytochemical and bioactive investigation of this plant led to the isolation of 18 alkaloids, of which 9 compounds inhibited the growth of Botrytis cinerea and 4 compounds against Penicillium italicum. The antifungal alkaloids could change the mycelium morphology, the total lipid content, and leak the cell contents of B. cinerea. Furthermore, the two most potent antifungal alkaloids, berberine (13) completely inhibited effect on gray mold of table grape at 512 mg L^-1, while jatrorrhizine (18) exhibited an inhibition rate > 90% on grape rot at the same concentration, with lower cytotoxicity and residue than chlorothalonil, which suggested that ingredients of M. fortunei might be a low-toxicity, low-residue, eco-friendly botanical fungicide against postharvest pathogens.

Key words: Mahonia fortunei, Chemical constituents, Botrytis cinerea, Penicillium italicum, Anti-postharvest pathogens

Xiao-Na Wang, Zhao-Jie Wang, Yun Zhao, Huan Wang, Mei-Ling Xiang, Yang-Yang Liu, Li-Xing Zhao, Xiao-Dong Luo. Antifungal alkaloids from Mahonia fortunei against pathogens of postharvest fruit[J]. 应用天然产物, 2023, 13(2): 10-10.

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Antifungal alkaloids from Mahonia fortunei against pathogens of postharvest fruit

Antifungal alkaloids from Mahonia fortunei against pathogens of postharvest fruit

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