The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans

doi:10.1007/s13659-015-0064-4

应用天然产物 ›› 2015, Vol. 5 ›› Issue (4): 177-183.DOI: 10.1007/s13659-015-0064-4

• Original article • 下一篇

The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans

Jie Yang^1,2,3, Qin-Li Wan^1,2, Quan-Zhang Mu¹, Chun-Feng Wu¹, Ai-Jun Ding^1,2, Zhong-Lin Yang^1,2, Ming-Hua Qiu¹, Huai-Rong Luo¹

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming 650201, Yunnan, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China

收稿日期:2015-04-28 修回日期:2015-05-25 出版日期:2015-08-24 发布日期:2018-02-11
通讯作者: Huai-Rong Luo,e-mail:luohuairong@mail.kib.ac.cn
基金资助:
This work was partially supported by the Yunnan provincial government(20080A007), and the 100 Talents Program of the Chinese Academy of Sciences, the State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany(P2008-ZZ21 and T2009-KF05).

The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans

Jie Yang^1,2,3, Qin-Li Wan^1,2, Quan-Zhang Mu¹, Chun-Feng Wu¹, Ai-Jun Ding^1,2, Zhong-Lin Yang^1,2, Ming-Hua Qiu¹, Huai-Rong Luo¹

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming 650201, Yunnan, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China

Received:2015-04-28 Revised:2015-05-25 Online:2015-08-24 Published:2018-02-11
Supported by:
This work was partially supported by the Yunnan provincial government(20080A007), and the 100 Talents Program of the Chinese Academy of Sciences, the State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany(P2008-ZZ21 and T2009-KF05).

摘要/Abstract

摘要： Aging is the major risk factor for many human diseases and degeneration. Thus, clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted. In traditional Chinese medicine(TCM), some were claimed to slow down aging. Qingyangshen(Cynanchum otophyllum schneid) is such a TCM. Here, we assayed the longevity effect of compound Otophylloside B(Ot B), a C-21 steroidal glycoside isolated from Qingyangshen, in Caenorhabditis elegans, which is a popular model for aging research. Our results showed that Ot B could modestly extend the lifespan of C. elegans, delay the age-related decline of body movement and improve the stress resistance. Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16. Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor(daf-2). In addition, Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis, for lifespan extension.

关键词: Otophylloside B, Caenorhabditis elegans, Aging, DAF-16/FOXO, IIS signaling pathway

Abstract: Aging is the major risk factor for many human diseases and degeneration. Thus, clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted. In traditional Chinese medicine(TCM), some were claimed to slow down aging. Qingyangshen(Cynanchum otophyllum schneid) is such a TCM. Here, we assayed the longevity effect of compound Otophylloside B(Ot B), a C-21 steroidal glycoside isolated from Qingyangshen, in Caenorhabditis elegans, which is a popular model for aging research. Our results showed that Ot B could modestly extend the lifespan of C. elegans, delay the age-related decline of body movement and improve the stress resistance. Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16. Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor(daf-2). In addition, Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis, for lifespan extension.

Key words: Otophylloside B, Caenorhabditis elegans, Aging, DAF-16/FOXO, IIS signaling pathway

Jie Yang, Qin-Li Wan, Quan-Zhang Mu, Chun-Feng Wu, Ai-Jun Ding, Zhong-Lin Yang, Ming-Hua Qiu, Huai-Rong Luo. The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans[J]. 应用天然产物, 2015, 5(4): 177-183.

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The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans

The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans

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