Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

doi:10.1007/s13659-024-00428-0

应用天然产物 ›› 2024, Vol. 14 ›› Issue (1): 10-10.DOI: 10.1007/s13659-024-00428-0

• ORIGINAL ARTICLES • 上一篇

Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

Shihui Qin^1,3, Yanlang Li³, Huiyan Shao³, Yang Yu¹, Yina Yang¹, Yi Zeng¹, Jia Huang³, Jiang-miao Hu³, Liu Yang^2,3

1. College of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, 230012, China;
2. State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, 999078, China;
3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China

收稿日期:2023-11-07 出版日期:2024-02-24 发布日期:2024-02-19
通讯作者: Jiang-miao Hu,E-mail:hujiangmiao@mail.kib.ac.cn;Liu Yang,E-mail:yangliu@mail.kib.ac.cn
基金资助:
The Chinese Academy of Sciences: KFJ-BRP-007-019, the authors declare no competing interests.

Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

Shihui Qin^1,3, Yanlang Li³, Huiyan Shao³, Yang Yu¹, Yina Yang¹, Yi Zeng¹, Jia Huang³, Jiang-miao Hu³, Liu Yang^2,3

1. College of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, 230012, China;
2. State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau, 999078, China;
3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China

Received:2023-11-07 Online:2024-02-24 Published:2024-02-19
Contact: Jiang-miao Hu,E-mail:hujiangmiao@mail.kib.ac.cn;Liu Yang,E-mail:yangliu@mail.kib.ac.cn
Supported by:
The Chinese Academy of Sciences: KFJ-BRP-007-019, the authors declare no competing interests.

摘要/Abstract

摘要： As the two most principal active substances in the corn silk, polysaccharides and flavonoids, the mechanism of interaction between them has been a topic of intense research. This study provides an in-depth investigation of the interaction mechanism between corn silk glycans and luteoloside (LUT) and the synergistic role that result from this interaction. The interaction mechanism was evaluated by isothermal titration calorimetry (ITC) and circular dichroism (CD), and the synergistic role was evaluated by the expression of glucose transporters (GLUT-1), insulin secretion and surface plasmon resonance (SPR). CD and ITC results indicated that the interaction between CSGs and LUT mainly driven by the Cotton effects, enthalpy and entropy-driven. This interaction precipitated the formation of complexes (CSGs/LUT complexes) between corn silk glycans (CSGs) with four different molecular weights and luteoloside (LUT). Furthermore, the CSGs and LUT play a synergistic role in glucose regulation through GLUT-1 expression and insulin secretion experiments, compared to single luteoloside group.

关键词: Corn silk glycans, CSGs/LUT complexes, The molecular interaction mechanism, The synergistic role

Abstract: As the two most principal active substances in the corn silk, polysaccharides and flavonoids, the mechanism of interaction between them has been a topic of intense research. This study provides an in-depth investigation of the interaction mechanism between corn silk glycans and luteoloside (LUT) and the synergistic role that result from this interaction. The interaction mechanism was evaluated by isothermal titration calorimetry (ITC) and circular dichroism (CD), and the synergistic role was evaluated by the expression of glucose transporters (GLUT-1), insulin secretion and surface plasmon resonance (SPR). CD and ITC results indicated that the interaction between CSGs and LUT mainly driven by the Cotton effects, enthalpy and entropy-driven. This interaction precipitated the formation of complexes (CSGs/LUT complexes) between corn silk glycans (CSGs) with four different molecular weights and luteoloside (LUT). Furthermore, the CSGs and LUT play a synergistic role in glucose regulation through GLUT-1 expression and insulin secretion experiments, compared to single luteoloside group.

Key words: Corn silk glycans, CSGs/LUT complexes, The molecular interaction mechanism, The synergistic role

Shihui Qin, Yanlang Li, Huiyan Shao, Yang Yu, Yina Yang, Yi Zeng, Jia Huang, Jiang-miao Hu, Liu Yang. Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity[J]. 应用天然产物, 2024, 14(1): 10-10.

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Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

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