Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways

doi:10.1007/s13659-023-00399-8

应用天然产物 ›› 2023, Vol. 13 ›› Issue (5): 33-33.DOI: 10.1007/s13659-023-00399-8

Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways

Donatus Wewura Adongo¹, Charles Kwaku Benneh¹, Augustine Tandoh¹, Robert Peter Biney², Kennedy Kwami Edem Kukuia³, Priscilla Kolibea Mante⁴, Benjamin Kingsley Harley⁵, David Oteng¹, Emmanuel Aduboffour Appiah¹, Ernest Cudjoe Anorbor¹, Eric Woode¹

1. Department of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana;
2. Department of Pharmacotherapeutics and Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana;
3. Department of Medical Pharmacology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Korle Bu, Accra, Ghana;
4. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;
5. Department of Pharmacognosy and Herbal Medicine, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana

收稿日期:2023-07-11 出版日期:2023-10-24 发布日期:2023-11-03
通讯作者: Donatus Wewura Adongo,E-mail:dadongo@uhas.edu.gh
基金资助:
We appreciate the technical support provided by Messrs. R. Agbolosoo and B. Cato of the Department of Pharmacology and Toxicology at the University of Health and Allied Sciences (UHAS).

Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways

1. Department of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana;
2. Department of Pharmacotherapeutics and Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana;
3. Department of Medical Pharmacology, University of Ghana Medical School, College of Health Sciences, University of Ghana, Korle Bu, Accra, Ghana;
4. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;
5. Department of Pharmacognosy and Herbal Medicine, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana

Received:2023-07-11 Online:2023-10-24 Published:2023-11-03
Contact: Donatus Wewura Adongo,E-mail:dadongo@uhas.edu.gh
Supported by:
We appreciate the technical support provided by Messrs. R. Agbolosoo and B. Cato of the Department of Pharmacology and Toxicology at the University of Health and Allied Sciences (UHAS).

摘要/Abstract

摘要： Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases. The hydroethanolic extract (PME) from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models. However, its effects in chronic anxiety models and possible mechanism(s) of action were not studied. Therefore, the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety. The zebrafish light dark test (LDT) and novel tank test (NTT) were employed to assess the anxiolytic-like effects of PME (0.1, 0.3, 1.0 mg mL^-1), fluoxetine (3×10^-5 mg mL^-1) and diazepam (1.5×10^-7 mg mL^-1). The chronic unpredictable stress (CUS) test was used to further evaluate the extract’s anxiolytic-like properties. The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil, granisetron, methysergide, or pizotifen, all at 1×10^-3 mg mL^-1. The extract significantly decreased anxiety behaviours in the NT and LD tests. These observed effects of the extract were however counteracted by flumazenil, granisetron, methysergide and pizotifen pre-treatment. In addition, PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish. Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways.

关键词: Anxiety disorders, Pseudospondias microcarpa, Zebrafish, Novel tank, Benzodiazepines

Abstract: Pseudospondias microcarpa is used in ethnomedicine to manage central nervous system diseases. The hydroethanolic extract (PME) from the leaves of the plant has shown anxiolytic-like properties in mice anxiety models. However, its effects in chronic anxiety models and possible mechanism(s) of action were not studied. Therefore, the current study evaluated the anxiolytic-like mechanisms of PME in zebrafish models of anxiety. The zebrafish light dark test (LDT) and novel tank test (NTT) were employed to assess the anxiolytic-like effects of PME (0.1, 0.3, 1.0 mg mL^-1), fluoxetine (3×10^-5 mg mL^-1) and diazepam (1.5×10^-7 mg mL^-1). The chronic unpredictable stress (CUS) test was used to further evaluate the extract’s anxiolytic-like properties. The potential mechanisms of anxiolytic action of the extract was evaluated after pre-treated with flumazenil, granisetron, methysergide, or pizotifen, all at 1×10^-3 mg mL^-1. The extract significantly decreased anxiety behaviours in the NT and LD tests. These observed effects of the extract were however counteracted by flumazenil, granisetron, methysergide and pizotifen pre-treatment. In addition, PME treatment significantly reversed CUS-induced anxiety behaviours in zebrafish. Results show that PME possesses anxiolytic-like effects possibly through interaction with serotonergic and gamma-aminobutyric acid mediated pathways.

Key words: Anxiety disorders, Pseudospondias microcarpa, Zebrafish, Novel tank, Benzodiazepines

Donatus Wewura Adongo, Charles Kwaku Benneh, Augustine Tandoh, Robert Peter Biney, Kennedy Kwami Edem Kukuia, Priscilla Kolibea Mante, Benjamin Kingsley Harley, David Oteng, Emmanuel Aduboffour Appiah, Ernest Cudjoe Anorbor, Eric Woode. Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways[J]. 应用天然产物, 2023, 13(5): 33-33.

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Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways

Anxiolytic-like effects of Pseudospondias microcarpa hydroethanolic leaf extract in zebrafish: Possible involvement of GABAergic and serotonergic pathways

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