The ion balance of Shotokuseki extract promotes filaggrin fragmentation and increases amino acid production and pyrrolidone carboxylic acid content in three-dimensional cultured human epidermis

doi:10.1007/s13659-022-00353-0

应用天然产物 ›› 2022, Vol. 12 ›› Issue (6): 37-37.DOI: 10.1007/s13659-022-00353-0

• ORIGINAL ARTICLES • 上一篇下一篇

The ion balance of Shotokuseki extract promotes filaggrin fragmentation and increases amino acid production and pyrrolidone carboxylic acid content in three-dimensional cultured human epidermis

Kei Tsukui^1,2, Takuya Kakiuchi³, Masamitsu Suzuki³, Hidetomo Sakurai³, Yoshihiro Tokudome^1,2,4

1 Laboratory of Cosmetic Sciences, Graduate School of Advanced Health Sciences, Saga University, 1 Honjo, Saga 840-8502, Japan;
2 Laboratory of Dermatological Physiology, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan;
3 Zeria Pharmaceutical Co., Ltd., 10-11 Nihonbashi, Kobuna-cho, Chuo-ku, Tokyo 103-8351, Japan;
4 Laboratory of Cosmetic Sciences, Regional Innovation Center, Saga University, 1 Honjo, Saga 840-8502, Japan

收稿日期:2022-04-28 出版日期:2022-12-24 发布日期:2022-12-23
通讯作者: Yoshihiro Tokudome, E-mail: domedome@cc.saga-u.ac.jp

The ion balance of Shotokuseki extract promotes filaggrin fragmentation and increases amino acid production and pyrrolidone carboxylic acid content in three-dimensional cultured human epidermis

Kei Tsukui^1,2, Takuya Kakiuchi³, Masamitsu Suzuki³, Hidetomo Sakurai³, Yoshihiro Tokudome^1,2,4

1 Laboratory of Cosmetic Sciences, Graduate School of Advanced Health Sciences, Saga University, 1 Honjo, Saga 840-8502, Japan;
2 Laboratory of Dermatological Physiology, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan;
3 Zeria Pharmaceutical Co., Ltd., 10-11 Nihonbashi, Kobuna-cho, Chuo-ku, Tokyo 103-8351, Japan;
4 Laboratory of Cosmetic Sciences, Regional Innovation Center, Saga University, 1 Honjo, Saga 840-8502, Japan

Received:2022-04-28 Online:2022-12-24 Published:2022-12-23
Contact: Yoshihiro Tokudome, E-mail: domedome@cc.saga-u.ac.jp

摘要/Abstract

摘要： Natural moisturizing factor (NMF) in the stratum corneum contributes to the retention of moisture there. The purpose of this study was to determine the penetration of ions in Shotokuseki extract (SE) into the three-dimensional cultured epidermis and the effect of NMF on the biosynthesis of amino acids and pyrrolidone carboxylic acid formation. Various ions, amino acids and pyrrolidone carboxylic acid were quantified by inductively coupled plasma mass spectrometry, fully automatic amino acid analyzer or high-performance liquid chromatography (HPLC) in three-dimensional cultured epidermis after application of SE. Gene expression levels of profilaggrin, calpain1, caspase14, and bleomycin hydrolase, which are involved in NMF production, were determined by reverse-transcription qPCR and bleomycin hydrolase activity was determined by aminopeptidase assay. The application of SE increased Na, K, Mg, Ca, Al, and Fe levels in three-dimensional cultured epidermis. The mRNA levels of the starting material of amino acid synthesis proflaggrin, and calpain1 and bleomycin hydrolase, which are involved in its fragmentation, increased. The activity of bleomycin hydrolase also increased. Furthermore, the levels of amino acids and pyrrolidone carboxylic acid increased in the three-dimensional cultured epidermis. This suggests that the ionic composition of SE may be involved in its moisturizing efect on the stratum corneum.

关键词: Shotokuseki extract, Epidermis, Amino acid, Pyrrolidone carboxylic acid, Ion, Gene expression

Abstract: Natural moisturizing factor (NMF) in the stratum corneum contributes to the retention of moisture there. The purpose of this study was to determine the penetration of ions in Shotokuseki extract (SE) into the three-dimensional cultured epidermis and the effect of NMF on the biosynthesis of amino acids and pyrrolidone carboxylic acid formation. Various ions, amino acids and pyrrolidone carboxylic acid were quantified by inductively coupled plasma mass spectrometry, fully automatic amino acid analyzer or high-performance liquid chromatography (HPLC) in three-dimensional cultured epidermis after application of SE. Gene expression levels of profilaggrin, calpain1, caspase14, and bleomycin hydrolase, which are involved in NMF production, were determined by reverse-transcription qPCR and bleomycin hydrolase activity was determined by aminopeptidase assay. The application of SE increased Na, K, Mg, Ca, Al, and Fe levels in three-dimensional cultured epidermis. The mRNA levels of the starting material of amino acid synthesis proflaggrin, and calpain1 and bleomycin hydrolase, which are involved in its fragmentation, increased. The activity of bleomycin hydrolase also increased. Furthermore, the levels of amino acids and pyrrolidone carboxylic acid increased in the three-dimensional cultured epidermis. This suggests that the ionic composition of SE may be involved in its moisturizing efect on the stratum corneum.

Key words: Shotokuseki extract, Epidermis, Amino acid, Pyrrolidone carboxylic acid, Ion, Gene expression

Kei Tsukui, Takuya Kakiuchi, Masamitsu Suzuki, Hidetomo Sakurai, Yoshihiro Tokudome. The ion balance of Shotokuseki extract promotes filaggrin fragmentation and increases amino acid production and pyrrolidone carboxylic acid content in three-dimensional cultured human epidermis[J]. 应用天然产物, 2022, 12(6): 37-37.

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The ion balance of Shotokuseki extract promotes filaggrin fragmentation and increases amino acid production and pyrrolidone carboxylic acid content in three-dimensional cultured human epidermis

The ion balance of Shotokuseki extract promotes filaggrin fragmentation and increases amino acid production and pyrrolidone carboxylic acid content in three-dimensional cultured human epidermis

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