Intestinal Absorption of Ergostane and Lanostane Triterpenoids from Antrodia cinnamomea Using Caco-2 Cell Monolayer Model

doi:10.1007/s13659-015-0072-4

应用天然产物 ›› 2015, Vol. 5 ›› Issue (5): 237-246.DOI: 10.1007/s13659-015-0072-4

• Original article • 上一篇下一篇

Intestinal Absorption of Ergostane and Lanostane Triterpenoids from Antrodia cinnamomea Using Caco-2 Cell Monolayer Model

Qi Wang¹, Xue Qiao¹, Yi Qian¹, Zi-wei Li¹, Yew-min Tzeng², De-min Zhou¹, De-an Guo¹, Min Ye¹

1. State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China;
2. Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung 41349, Taiwan

收稿日期:2015-08-11 修回日期:2015-09-10 出版日期:2015-10-24 发布日期:2018-02-11
通讯作者: De-min Zhou,e-mail:deminzhou@bjmu.edu.cn;Min Ye,e-mail:yemin@bjmu.edu.cn
基金资助:
This work was supported by National Natural Science Foundation of China(Nos. 81222054, 81303294), and the Program for New Century Excellent Talents in University from Chinese Ministry of Education(No. NCET-11-0019).

Intestinal Absorption of Ergostane and Lanostane Triterpenoids from Antrodia cinnamomea Using Caco-2 Cell Monolayer Model

Qi Wang¹, Xue Qiao¹, Yi Qian¹, Zi-wei Li¹, Yew-min Tzeng², De-min Zhou¹, De-an Guo¹, Min Ye¹

1. State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China;
2. Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung 41349, Taiwan

Received:2015-08-11 Revised:2015-09-10 Online:2015-10-24 Published:2018-02-11
Supported by:
This work was supported by National Natural Science Foundation of China(Nos. 81222054, 81303294), and the Program for New Century Excellent Talents in University from Chinese Ministry of Education(No. NCET-11-0019).

摘要/Abstract

摘要： Antrodia cinnamomea is a precious medicinal mushroom. It exhibits promising therapeutic effects on cancer, intoxication, hypertension, hepatitis, and inflammation. Its major bioactive constituents are ergostane and lanostane triterpenoids. In this study, we used intestinal Caco-2 cell monolayer model to reveal the intestinal absorption property of 14 representative triterpenoids from A. cinnamomea. The bidirectional transport through the monolayer at different time points was monitored by a fully validated LC/MS/MS method. In the case of pure compounds, ergostanes 5(25R-antcin H), 6(25S-antcin H) and 10(25R-antcin B) could readily pass through the Caco-2 cell layer, whereas lanostanes 13(dehydroeburicoic acid) and 14(eburicoic acid) could hardly pass through. When the cells were treated with A. cinnamomea extract, antcins A, B, C, H and K(1-6 and 9-11) were absorbed via passive transcellular diffusion, and showed high P_AB and P_BA values(>2.5×10^-5 cm/s). Meanwhile, the lanostanes dehydrosulphurenic acid(8), 15α-acetyldehydrosulphurenic acid(12), 13 and 14 exhibited poor permeability. Transport features of these compounds were consistent with their pharmacokinetic behaviors in rats. This study could also be helpful in predicting the intestinal absorption of A. cinnamomea in human.

关键词: Antrodia cinnamomea, Ergostane, Lanostane, Triterpenoids, Caco-2

Abstract: Antrodia cinnamomea is a precious medicinal mushroom. It exhibits promising therapeutic effects on cancer, intoxication, hypertension, hepatitis, and inflammation. Its major bioactive constituents are ergostane and lanostane triterpenoids. In this study, we used intestinal Caco-2 cell monolayer model to reveal the intestinal absorption property of 14 representative triterpenoids from A. cinnamomea. The bidirectional transport through the monolayer at different time points was monitored by a fully validated LC/MS/MS method. In the case of pure compounds, ergostanes 5(25R-antcin H), 6(25S-antcin H) and 10(25R-antcin B) could readily pass through the Caco-2 cell layer, whereas lanostanes 13(dehydroeburicoic acid) and 14(eburicoic acid) could hardly pass through. When the cells were treated with A. cinnamomea extract, antcins A, B, C, H and K(1-6 and 9-11) were absorbed via passive transcellular diffusion, and showed high P_AB and P_BA values(>2.5×10^-5 cm/s). Meanwhile, the lanostanes dehydrosulphurenic acid(8), 15α-acetyldehydrosulphurenic acid(12), 13 and 14 exhibited poor permeability. Transport features of these compounds were consistent with their pharmacokinetic behaviors in rats. This study could also be helpful in predicting the intestinal absorption of A. cinnamomea in human.

Key words: Antrodia cinnamomea, Ergostane, Lanostane, Triterpenoids, Caco-2

Qi Wang, Xue Qiao, Yi Qian, Zi-wei Li, Yew-min Tzeng, De-min Zhou, De-an Guo, Min Ye. Intestinal Absorption of Ergostane and Lanostane Triterpenoids from Antrodia cinnamomea Using Caco-2 Cell Monolayer Model[J]. 应用天然产物, 2015, 5(5): 237-246.

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Intestinal Absorption of Ergostane and Lanostane Triterpenoids from Antrodia cinnamomea Using Caco-2 Cell Monolayer Model

Intestinal Absorption of Ergostane and Lanostane Triterpenoids from Antrodia cinnamomea Using Caco-2 Cell Monolayer Model

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