Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant

doi:10.1007/s13659-012-0035-y

应用天然产物 ›› 2012, Vol. 2 ›› Issue (4): 160-165.DOI: 10.1007/s13659-012-0035-y

• Regular Article • 上一篇下一篇

Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant

Ranabir SAHU, Saikat DEWANJEE

Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India

收稿日期:2012-05-11 修回日期:2012-05-11 出版日期:2012-08-24 发布日期:2018-02-11
通讯作者: Saikat DEWANJEE,E-mail:s.dewanjee@yahoo.com
基金资助:
The authors are thankful to University Grants Commission, New Delhi, India for providing fund to Mr Ranabir Sahu through Rajiv Gandhi National Fellowship, 2011.

Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant

Ranabir SAHU, Saikat DEWANJEE

Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India

Received:2012-05-11 Revised:2012-05-11 Online:2012-08-24 Published:2018-02-11
Supported by:
The authors are thankful to University Grants Commission, New Delhi, India for providing fund to Mr Ranabir Sahu through Rajiv Gandhi National Fellowship, 2011.

摘要/Abstract

摘要： Solenostemon scutellarioides is a commercially important ornamental plant. In present study, it was attempted to establish a protocol for high frequency in vitro regeneration of S. scutellarioides. Caulogenesis was found to be significant in solid MS medium supplemented with 0.5 mg dm^-3 BAP. Combination of GA₃(0.5 mg dm^-3) and BAP(0.5 mg dm^-3) induced high frequency regeneration coupled with higher plant height. The plantlets exposed to IBA(1 mg dm^-3) exhibited significant root development in terms of first appearance of root and number of roots per shoot. To improve its commercial acceptability in terms of plant architecture and foliage colouration, two weeks old in vitro grown plantlets were exposed to different culture conditions namely MS strength, sucrose concentration, pH and light. A variable plant types with diverse ornamental traits were developed under different culture conditions.

关键词: anthocyanin, chlorophyll, in vitro, micropropagation, Solenostemon scutellarioides

Abstract: Solenostemon scutellarioides is a commercially important ornamental plant. In present study, it was attempted to establish a protocol for high frequency in vitro regeneration of S. scutellarioides. Caulogenesis was found to be significant in solid MS medium supplemented with 0.5 mg dm^-3 BAP. Combination of GA₃(0.5 mg dm^-3) and BAP(0.5 mg dm^-3) induced high frequency regeneration coupled with higher plant height. The plantlets exposed to IBA(1 mg dm^-3) exhibited significant root development in terms of first appearance of root and number of roots per shoot. To improve its commercial acceptability in terms of plant architecture and foliage colouration, two weeks old in vitro grown plantlets were exposed to different culture conditions namely MS strength, sucrose concentration, pH and light. A variable plant types with diverse ornamental traits were developed under different culture conditions.

Key words: anthocyanin, chlorophyll, in vitro, micropropagation, Solenostemon scutellarioides

Ranabir SAHU, Saikat DEWANJEE. Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant[J]. 应用天然产物, 2012, 2(4): 160-165.

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Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant

Differential physiological and biochemical responses under variable culture conditions in micro-propagated Solenostemon scutellarioides: an important ornamental plant

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