Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

doi:10.1007/s13659-012-0017-0

应用天然产物 ›› 2012, Vol. 2 ›› Issue (2): 87-91.DOI: 10.1007/s13659-012-0017-0

• Regular Article • 上一篇

Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

Ramona LIEDER^a,b, Sigrídur Thóra REYNISDÓTTIR^a, Finnbogi THORMÓDSSON^e, Chuen-How NG^c, Jon Magnús EINARSSON^c, Jóhannes GÍSLASON^c, Jóhannes BJÖRNSSON^d,f, Sveinn GUDMUNDSSON^a, Pétur Henry PETERSEN^e, Ólafur Eysteinn SIGURJÓNSSON^a,b,d

a The Blood Bank, Landspitali University Hospital, Snorrabraut 60, 105 Reykjavik, Iceland;
b School of Science and Engineering, Reykjavik University, Menntavegi 1, 101 Reykjavik, Iceland;
c Genis ehf, Vatnagördum 18, 104 Reykjavik, Iceland;
d Biomedical Center, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
e Department of Anatomy, Medical Faculty, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
f Department of Pathology, Landspitali University Hospital, Baronsstig, 101 Reykjavik, Iceland

收稿日期:2012-02-28 修回日期:2012-03-25 出版日期:2012-04-24 发布日期:2018-02-11
通讯作者: Ólafur Eysteinn SIGURJÓNSSON,E-mail:oes@landspitali.is
基金资助:
This work was supported by grants from Technology Development Fund, managed by the Icelandic Center for Research, and Landspitali University Hospital Research Fund.

Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

a The Blood Bank, Landspitali University Hospital, Snorrabraut 60, 105 Reykjavik, Iceland;
b School of Science and Engineering, Reykjavik University, Menntavegi 1, 101 Reykjavik, Iceland;
c Genis ehf, Vatnagördum 18, 104 Reykjavik, Iceland;
d Biomedical Center, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
e Department of Anatomy, Medical Faculty, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
f Department of Pathology, Landspitali University Hospital, Baronsstig, 101 Reykjavik, Iceland

Received:2012-02-28 Revised:2012-03-25 Online:2012-04-24 Published:2018-02-11
Supported by:
This work was supported by grants from Technology Development Fund, managed by the Icelandic Center for Research, and Landspitali University Hospital Research Fund.

摘要/Abstract

摘要： Human mesenchymal stem cells(hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins(CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes.

关键词: YKL-40, mesenchymal stem cells, osteogenic differentiation, chitinase-like-protein

Abstract: Human mesenchymal stem cells(hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins(CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes.

Key words: YKL-40, mesenchymal stem cells, osteogenic differentiation, chitinase-like-protein

Ramona LIEDER, Sigrídur Thóra REYNISDÓTTIR, Finnbogi THORMÓDSSON, Chuen-How NG, Jon Magnús EINARSSON, Jóhannes GÍSLASON, Jóhannes BJÖRNSSON, Sveinn GUDMUNDSSON, Pétur Henry PETERSEN, Ólafur Eysteinn SIGURJÓNSSON. Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation[J]. 应用天然产物, 2012, 2(2): 87-91.

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Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

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