Identification of enzymes responsible for the reduction of geraniol to citronellol

doi:10.1007/s13659-011-0032-6

应用天然产物 ›› 2011, Vol. 1 ›› Issue (3): 108-111.DOI: 10.1007/s13659-011-0032-6

• Regular Article • 上一篇下一篇

Identification of enzymes responsible for the reduction of geraniol to citronellol

Tian-Tian YUAN^a,b, Qian-Qian CHEN^a, Pei-Ji ZHAO^a, Ying ZENG^a, Xiao-Zhu LIU^b, Shan LU^c

a State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
b Life Science College, Southwest Forestry University, Kunming 650224, China;
c State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China

收稿日期:2011-11-17 修回日期:2011-11-30 出版日期:2011-06-24 发布日期:2018-02-11
通讯作者: Ying ZENG,E-mail:biochem@mail.kib.ac.cn
基金资助:
This work was supported by grants from the Ministry of Science and Technology of China(2007CB108802) and State Key Laboratory of Phytochemistry and Plant Resources in West China(P2008-ZZ16, P2009-ZZ02).

Identification of enzymes responsible for the reduction of geraniol to citronellol

Tian-Tian YUAN^a,b, Qian-Qian CHEN^a, Pei-Ji ZHAO^a, Ying ZENG^a, Xiao-Zhu LIU^b, Shan LU^c

a State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
b Life Science College, Southwest Forestry University, Kunming 650224, China;
c State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China

Received:2011-11-17 Revised:2011-11-30 Online:2011-06-24 Published:2018-02-11
Supported by:
This work was supported by grants from the Ministry of Science and Technology of China(2007CB108802) and State Key Laboratory of Phytochemistry and Plant Resources in West China(P2008-ZZ16, P2009-ZZ02).

摘要/Abstract

摘要： The reduction of geraniol to citronellol is the first step for the synthesis of natural phytol in the production of tocopherols and natural vitamin K. Baker's yeast was used in the bioreduction described above as a whole-cell biocatalyst. However, the enzyme responsible for the reduction of geraniol to citronellol is not yet known. Four old yellow enzyme(OYE) genes were cloned from yeast and plants, and expressed in Escherichia coli for a high level of recombinant proteins. The recombinant protein displayed a catalytic activity of converting geraniol to citronellol as a sole product verified by GC-MS analyses. The recombinant OYE2 intact cells were found to show 3. 7 and 1. 9-fold higher activity than that of yeast cells and the recombinant crude extracts, respectively. Compared to the recombinant fusion enzyme, the entrokinase-cleaved enzyme displayed nearly identical activity for geraniol reduction. To our knowledge, this is the first enzyme identified to catalyze the formation of citronellol from geraniol by reducing the allylic alcohol double bond, which is normally known as inactivating group for the old yellow enzymes.

关键词: allylic alcohol, citronellol, geraniol, old yellow enzyme, reduction

Abstract: The reduction of geraniol to citronellol is the first step for the synthesis of natural phytol in the production of tocopherols and natural vitamin K. Baker's yeast was used in the bioreduction described above as a whole-cell biocatalyst. However, the enzyme responsible for the reduction of geraniol to citronellol is not yet known. Four old yellow enzyme(OYE) genes were cloned from yeast and plants, and expressed in Escherichia coli for a high level of recombinant proteins. The recombinant protein displayed a catalytic activity of converting geraniol to citronellol as a sole product verified by GC-MS analyses. The recombinant OYE2 intact cells were found to show 3. 7 and 1. 9-fold higher activity than that of yeast cells and the recombinant crude extracts, respectively. Compared to the recombinant fusion enzyme, the entrokinase-cleaved enzyme displayed nearly identical activity for geraniol reduction. To our knowledge, this is the first enzyme identified to catalyze the formation of citronellol from geraniol by reducing the allylic alcohol double bond, which is normally known as inactivating group for the old yellow enzymes.

Key words: allylic alcohol, citronellol, geraniol, old yellow enzyme, reduction

Tian-Tian YUAN, Qian-Qian CHEN, Pei-Ji ZHAO, Ying ZENG, Xiao-Zhu LIU, Shan LU. Identification of enzymes responsible for the reduction of geraniol to citronellol[J]. 应用天然产物, 2011, 1(3): 108-111.

Tian-Tian YUAN, Qian-Qian CHEN, Pei-Ji ZHAO, Ying ZENG, Xiao-Zhu LIU, Shan LU. Identification of enzymes responsible for the reduction of geraniol to citronellol[J]. Natural Products and Bioprospecting, 2011, 1(3): 108-111.

Identification of enzymes responsible for the reduction of geraniol to citronellol

Identification of enzymes responsible for the reduction of geraniol to citronellol

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价