Metabolomic and transcriptomic analyses revealed potential mechanisms of Anchusa italica Retz. in alleviating cerebral ischemia-reperfusion injury via Wnt/β-catenin pathway modulation

doi:10.1007/s13659-024-00495-3

应用天然产物 ›› 2025, Vol. 15 ›› Issue (2): 11-11.DOI: 10.1007/s13659-024-00495-3

• ORIGINAL ARTICLES • 下一篇

Metabolomic and transcriptomic analyses revealed potential mechanisms of Anchusa italica Retz. in alleviating cerebral ischemia-reperfusion injury via Wnt/β-catenin pathway modulation

Wenta Tan^1,2, Shuo Fu¹, Yufei Wang³, Bojun Hu⁴, Guiquan Ding¹, Li Zhang^1,5, Wen Zhang¹, Guanhua Du^1,6, Junke Song¹

1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China;
2. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China;
3. Qingdao Center for Disease Control and Prevention, Qingdao, 266000, China;
4. School of Pharmacy, Henan University, Kaifeng, 475004, China;
5. Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uyghur Medicine, Urumqi, 830000, China;
6. Xinjiang Key Laboratory of Uygur Medical Research, Xinjiang Institute of Materia Medica, Urumqi, 830004, China

收稿日期:2024-10-20 接受日期:2024-12-20 出版日期:2025-04-25 发布日期:2025-05-17
通讯作者: Wen Zhang, E-mail:zhangwen9010@imm.ac.cn;Guanhua Du, E-mail:dugh@imm.ac.cn;Junke Song, E-mail:smilejunke@imm.ac.cn
基金资助:
This research was funded by the National Natural Science Foundation of China (No. 82004071), the CAMS Innovation Fund for Medical Sciences (CIFMS) (No. 2022-I2M-1-015), the Young Elite Scientist Sponsorship Program by China Association for Science and Technology (No. YESS20200108), and Xinjiang Uygur Autonomous Region Innovation Environment Construction Special Fund and Technology Innovation Base Construction Key Laboratory Open Project (No. 2023D04065).

Metabolomic and transcriptomic analyses revealed potential mechanisms of Anchusa italica Retz. in alleviating cerebral ischemia-reperfusion injury via Wnt/β-catenin pathway modulation

Wenta Tan^1,2, Shuo Fu¹, Yufei Wang³, Bojun Hu⁴, Guiquan Ding¹, Li Zhang^1,5, Wen Zhang¹, Guanhua Du^1,6, Junke Song¹

1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China;
2. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China;
3. Qingdao Center for Disease Control and Prevention, Qingdao, 266000, China;
4. School of Pharmacy, Henan University, Kaifeng, 475004, China;
5. Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uyghur Medicine, Urumqi, 830000, China;
6. Xinjiang Key Laboratory of Uygur Medical Research, Xinjiang Institute of Materia Medica, Urumqi, 830004, China

Received:2024-10-20 Accepted:2024-12-20 Online:2025-04-25 Published:2025-05-17
Contact: Wen Zhang, E-mail:zhangwen9010@imm.ac.cn;Guanhua Du, E-mail:dugh@imm.ac.cn;Junke Song, E-mail:smilejunke@imm.ac.cn
Supported by:
This research was funded by the National Natural Science Foundation of China (No. 82004071), the CAMS Innovation Fund for Medical Sciences (CIFMS) (No. 2022-I2M-1-015), the Young Elite Scientist Sponsorship Program by China Association for Science and Technology (No. YESS20200108), and Xinjiang Uygur Autonomous Region Innovation Environment Construction Special Fund and Technology Innovation Base Construction Key Laboratory Open Project (No. 2023D04065).

摘要/Abstract

摘要： Anchusa italica Retz. (AIR), a traditional herbal remedy, is commonly applied in managing heart and brain disorders. However, its specific function and mechanism in acute cerebral ischemia-reperfusion injury (CIRI) are not fully understood. This research focused on the interventional effects and potential mechanisms of AIR extract (AIRE) in a rat model of CIRI. The model was established using the filament occlusion method, which involved blocking the middle cerebral artery for 1.5 h and then removing the filament to restore blood flow. Transcriptomic and metabolomic analyses were conducted to explore the molecular pathways and metabolites affected by AIRE. ATP level was measured using an ATP assay kit. Additionally, RT-qPCR and western blot tests were conducted to evaluate the influence of AIRE on the Wnt signaling pathway and mitochondrial function. Transcriptomic and metabolomic analyses indicated that AIRE regulated the Wnt signaling pathway in CIRI and modulated metabolites associated with mitochondrial energy metabolism, such as citrate and succinate. ATP assay result demonstrated that AIRE enhanced ATP production in CIRI. Further, RT-qPCR and western blot analyses revealed that AIRE activated the Wnt/β-catenin signaling pathway and corrected mitochondrial dysfunction. These results proposed that AIRE mitigated mitochondrial energy metabolism deficits in CIRI via the Wnt/β-catenin pathway. By restoring the balance of mitochondrial function and energy metabolism, AIRE might offer a potentially therapeutic strategy for addressing CIRI.

关键词: Anchusa italica Retz., Cerebral ischemia-reperfusion injury, Transcriptomics, Metabolomics, Wnt/β-catenin signaling pathway

Abstract: Anchusa italica Retz. (AIR), a traditional herbal remedy, is commonly applied in managing heart and brain disorders. However, its specific function and mechanism in acute cerebral ischemia-reperfusion injury (CIRI) are not fully understood. This research focused on the interventional effects and potential mechanisms of AIR extract (AIRE) in a rat model of CIRI. The model was established using the filament occlusion method, which involved blocking the middle cerebral artery for 1.5 h and then removing the filament to restore blood flow. Transcriptomic and metabolomic analyses were conducted to explore the molecular pathways and metabolites affected by AIRE. ATP level was measured using an ATP assay kit. Additionally, RT-qPCR and western blot tests were conducted to evaluate the influence of AIRE on the Wnt signaling pathway and mitochondrial function. Transcriptomic and metabolomic analyses indicated that AIRE regulated the Wnt signaling pathway in CIRI and modulated metabolites associated with mitochondrial energy metabolism, such as citrate and succinate. ATP assay result demonstrated that AIRE enhanced ATP production in CIRI. Further, RT-qPCR and western blot analyses revealed that AIRE activated the Wnt/β-catenin signaling pathway and corrected mitochondrial dysfunction. These results proposed that AIRE mitigated mitochondrial energy metabolism deficits in CIRI via the Wnt/β-catenin pathway. By restoring the balance of mitochondrial function and energy metabolism, AIRE might offer a potentially therapeutic strategy for addressing CIRI.

Key words: Anchusa italica Retz., Cerebral ischemia-reperfusion injury, Transcriptomics, Metabolomics, Wnt/β-catenin signaling pathway

Wenta Tan, Shuo Fu, Yufei Wang, Bojun Hu, Guiquan Ding, Li Zhang, Wen Zhang, Guanhua Du, Junke Song. Metabolomic and transcriptomic analyses revealed potential mechanisms of Anchusa italica Retz. in alleviating cerebral ischemia-reperfusion injury via Wnt/β-catenin pathway modulation[J]. 应用天然产物, 2025, 15(2): 11-11.

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Metabolomic and transcriptomic analyses revealed potential mechanisms of Anchusa italica Retz. in alleviating cerebral ischemia-reperfusion injury via Wnt/β-catenin pathway modulation

Metabolomic and transcriptomic analyses revealed potential mechanisms of Anchusa italica Retz. in alleviating cerebral ischemia-reperfusion injury via Wnt/β-catenin pathway modulation

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