Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway

doi:10.1007/s13659-024-00482-8

应用天然产物 ›› 2024, Vol. 14 ›› Issue (6): 60-60.DOI: 10.1007/s13659-024-00482-8

• ORIGINAL ARTICLES • 上一篇下一篇

Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway

Qiyuan Su¹, Qian Hu², Songtao Wu³, Suqin Yang⁴, Hanwen Su⁵, Zhengjun Zhang^6,7, Chengxiu Ling¹

1. Wisdom Lake Academy of Pharmacy, Xi'an Jiaotong-Liverpool University, Suzhou 215123, Jiangsu, People's Republic of China;
2. Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430016, Hubei, People's Republic of China;
3. Faculty of Pharmacy, Hubei University of Chinese Medicine, No. 16, Huangjiahu West Road, Hongshan District, Wuhan 430065, Hubei, People's Republic of China;
4. School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, Hubei, People's Republic of China;
5. Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, People's Republic of China;
6. Department of Statistics, University of Wisconsin-Madison, Madison, WI 53706-1481, USA;
7. School of Economics and Management, and MOE Social Science Laboratory of Digital Economic Forecasts and Policy Simulation, University of Chinese Academy of Sciences, Center for Forecasting Sciences, Chinese Academy of Sciences, Beijing 100049, People's Republic of China

收稿日期:2024-09-21 出版日期:2024-12-24 发布日期:2024-12-13
通讯作者: Hanwen Su,E-mail:hanwensu@whu.edu.cn;Zhengjun Zhang,E-mail:zjz@stat.wisc.edu;Chengxiu Ling,E-mail:Chengxiu.ling@xjtlu.edu.cn
基金资助:
This work was supported by the Natural Science Foundation of Hubei Province (2024AFD252); the Fundamental Research Funds for the Central Universities South-Central Minzu University (Grant Numbers: CZZ24017); and the Fundamental Research Funds for Health Commission of Hubei Province (ZY2023M022).

Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway

Qiyuan Su¹, Qian Hu², Songtao Wu³, Suqin Yang⁴, Hanwen Su⁵, Zhengjun Zhang^6,7, Chengxiu Ling¹

1. Wisdom Lake Academy of Pharmacy, Xi'an Jiaotong-Liverpool University, Suzhou 215123, Jiangsu, People's Republic of China;
2. Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430016, Hubei, People's Republic of China;
3. Faculty of Pharmacy, Hubei University of Chinese Medicine, No. 16, Huangjiahu West Road, Hongshan District, Wuhan 430065, Hubei, People's Republic of China;
4. School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, Hubei, People's Republic of China;
5. Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, People's Republic of China;
6. Department of Statistics, University of Wisconsin-Madison, Madison, WI 53706-1481, USA;
7. School of Economics and Management, and MOE Social Science Laboratory of Digital Economic Forecasts and Policy Simulation, University of Chinese Academy of Sciences, Center for Forecasting Sciences, Chinese Academy of Sciences, Beijing 100049, People's Republic of China

Received:2024-09-21 Online:2024-12-24 Published:2024-12-13
Contact: Hanwen Su,E-mail:hanwensu@whu.edu.cn;Zhengjun Zhang,E-mail:zjz@stat.wisc.edu;Chengxiu Ling,E-mail:Chengxiu.ling@xjtlu.edu.cn
Supported by:
This work was supported by the Natural Science Foundation of Hubei Province (2024AFD252); the Fundamental Research Funds for the Central Universities South-Central Minzu University (Grant Numbers: CZZ24017); and the Fundamental Research Funds for Health Commission of Hubei Province (ZY2023M022).

摘要/Abstract

摘要： This study aimed to evaluate the therapeutic properties of the traditional Chinese medicine Xuesanqi (XSQ, from the rhizome of Polygonum amplexicaule D. Don) in treating ulcerative colitis. We hypothesized that its many active components can alleviate symptoms of colitis by regulating the gut microbiota, its metabolites, and various signaling pathways. To test our hypotheses, we designed a DSS- induced colitis model in C57BL/6 male mice. Apparent metrics were evaluated in each group of mice and performed histological analysis of relevant tissues. The gut microbial composition was analyzed by 16S rRNA sequencing of bacteria. Simultaneously, the SCFAs content was detected by gas chromatography, inflammatory factor secretion was evaluated by ELISA or western-blot, the expression of tight junction protein and key proteins of the MAPK signaling pathway were analyzed by western-blot. Our result showed that the treatment with XSQ alleviated significant various symptoms such as weight loss, blood in stool, and shortening of colon. In addition, XSQ treatment restored the dysregulated gut microbiota in colitis mice, increased short chain fatty acids (SCFAs) and normalized the MAPK/ERK/JNK signaling pathways, promoted expression of tight junction protein Occludin, Claudin-1, and E-cadherin proteins. Furthermore, we also observed a dose-dependent pattern in these treatment responses. These findings demonstrated the active components of XSQ is a promising new treatment platform for ulcerative colitis.

关键词: Colitis, Xuesanqi, Gut microbiota, Short chain fatty acids (SCFas), MAPK/ERK/JNK signaling pathway, Tight junction (T.J.) protein

Abstract: This study aimed to evaluate the therapeutic properties of the traditional Chinese medicine Xuesanqi (XSQ, from the rhizome of Polygonum amplexicaule D. Don) in treating ulcerative colitis. We hypothesized that its many active components can alleviate symptoms of colitis by regulating the gut microbiota, its metabolites, and various signaling pathways. To test our hypotheses, we designed a DSS- induced colitis model in C57BL/6 male mice. Apparent metrics were evaluated in each group of mice and performed histological analysis of relevant tissues. The gut microbial composition was analyzed by 16S rRNA sequencing of bacteria. Simultaneously, the SCFAs content was detected by gas chromatography, inflammatory factor secretion was evaluated by ELISA or western-blot, the expression of tight junction protein and key proteins of the MAPK signaling pathway were analyzed by western-blot. Our result showed that the treatment with XSQ alleviated significant various symptoms such as weight loss, blood in stool, and shortening of colon. In addition, XSQ treatment restored the dysregulated gut microbiota in colitis mice, increased short chain fatty acids (SCFAs) and normalized the MAPK/ERK/JNK signaling pathways, promoted expression of tight junction protein Occludin, Claudin-1, and E-cadherin proteins. Furthermore, we also observed a dose-dependent pattern in these treatment responses. These findings demonstrated the active components of XSQ is a promising new treatment platform for ulcerative colitis.

Key words: Colitis, Xuesanqi, Gut microbiota, Short chain fatty acids (SCFas), MAPK/ERK/JNK signaling pathway, Tight junction (T.J.) protein

Qiyuan Su, Qian Hu, Songtao Wu, Suqin Yang, Hanwen Su, Zhengjun Zhang, Chengxiu Ling. Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway[J]. 应用天然产物, 2024, 14(6): 60-60.

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Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway

Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway

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