Paeoniflorin mitigates insulin-like growth factor 1-induced lipogenesis and inflammation in human sebocytes by inhibiting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways

doi:10.1007/s13659-024-00478-4

应用天然产物 ›› 2024, Vol. 14 ›› Issue (6): 56-56.DOI: 10.1007/s13659-024-00478-4

Paeoniflorin mitigates insulin-like growth factor 1-induced lipogenesis and inflammation in human sebocytes by inhibiting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways

Chuanchuan Cai¹, Si Liu^2,3, Yufeng Liu^2,3, Shaobin Huang^2,3, Shiya Lu⁴, Fang Liu^2,3, Xiaohua Luo^2,3, Christos C. Zouboulis⁵, Ge Shi^2,3

1. Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China;
2. Department of Cosmetic and Plastic Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China;
3. Biomedical Innovation Center, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China;
4. Huamei-Bond International College, Guangzhou, 510520, China;
5. Departments of Dermatology, Venereology, Allergology and Immunology, Staedtisches Klinikum Dessau, Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg, 06847, Dessau, Germany

收稿日期:2024-07-18 出版日期:2024-12-24 发布日期:2024-12-13
通讯作者: Ge Shi,E-mail:shig9@mail.sysu.edu.cn
基金资助:
This work was s financially supported by the National Natural Science Foundation of China (Project No. 81773345 and 82104856) and Basic and Applied Basic Research Foundation of Guangdong Province (No. SL2023A04J01298).

Paeoniflorin mitigates insulin-like growth factor 1-induced lipogenesis and inflammation in human sebocytes by inhibiting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways

Chuanchuan Cai¹, Si Liu^2,3, Yufeng Liu^2,3, Shaobin Huang^2,3, Shiya Lu⁴, Fang Liu^2,3, Xiaohua Luo^2,3, Christos C. Zouboulis⁵, Ge Shi^2,3

1. Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China;
2. Department of Cosmetic and Plastic Surgery, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China;
3. Biomedical Innovation Center, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China;
4. Huamei-Bond International College, Guangzhou, 510520, China;
5. Departments of Dermatology, Venereology, Allergology and Immunology, Staedtisches Klinikum Dessau, Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg, 06847, Dessau, Germany

Received:2024-07-18 Online:2024-12-24 Published:2024-12-13
Contact: Ge Shi,E-mail:shig9@mail.sysu.edu.cn
Supported by:
This work was s financially supported by the National Natural Science Foundation of China (Project No. 81773345 and 82104856) and Basic and Applied Basic Research Foundation of Guangdong Province (No. SL2023A04J01298).

摘要/Abstract

摘要： Insulin-like growth factor-1 (IGF-1) is considered as a pathogenic factor contributing to sebaceous gland dysfunction, which leads to acne vulgaris. Paeoniflorin (Pae), a bioactive monomer derived from total glycosides of paeony, has shown potential in treating various diseases. However, its anti-acne effects on human sebocytes are not well understood. In this study, we investigated the effects of Pae on acne development induced by IGF-1 in SZ95 sebocytes. Following IGF-1 stimulation, SZ95 sebocytes were exposed to Pae and then determined for proliferation, cell cycle, apoptosis, lipogenesis and pro-inflammatory cytokine secretion. We also analyzed the expression of proteins involved in the PI3K/Akt/FoxO1 and JAK2/STAT3 pathways. In vitro experiments demonstrated that Pae significantly inhibited colony formation, induced G1/S cell cycle arrest, promoted apoptosis, inhibited lipogenesis and cytokine synthesis in IGF-1-treated SZ95 sebocytes. Furthermore, Pae suppressed the phosphorylation of Akt, FoxO1, JAK2, and STAT3. Importantly, the sebo-suppressive and anti-inflammatory effects of Pae were enhanced by blocking PI3K and JAK2. In summary, our findings suggest that Pae has potent anti-proliferative and pro-apoptotic effects in SZ95 sebocytes. Additionally, Pae effectively protects against IGF-1-induced lipogenesis and inflammation by targeting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways.

关键词: Paeoniflorin, Acne vulgaris, Sebocytes, Insulin-like growth factor-1

Abstract: Insulin-like growth factor-1 (IGF-1) is considered as a pathogenic factor contributing to sebaceous gland dysfunction, which leads to acne vulgaris. Paeoniflorin (Pae), a bioactive monomer derived from total glycosides of paeony, has shown potential in treating various diseases. However, its anti-acne effects on human sebocytes are not well understood. In this study, we investigated the effects of Pae on acne development induced by IGF-1 in SZ95 sebocytes. Following IGF-1 stimulation, SZ95 sebocytes were exposed to Pae and then determined for proliferation, cell cycle, apoptosis, lipogenesis and pro-inflammatory cytokine secretion. We also analyzed the expression of proteins involved in the PI3K/Akt/FoxO1 and JAK2/STAT3 pathways. In vitro experiments demonstrated that Pae significantly inhibited colony formation, induced G1/S cell cycle arrest, promoted apoptosis, inhibited lipogenesis and cytokine synthesis in IGF-1-treated SZ95 sebocytes. Furthermore, Pae suppressed the phosphorylation of Akt, FoxO1, JAK2, and STAT3. Importantly, the sebo-suppressive and anti-inflammatory effects of Pae were enhanced by blocking PI3K and JAK2. In summary, our findings suggest that Pae has potent anti-proliferative and pro-apoptotic effects in SZ95 sebocytes. Additionally, Pae effectively protects against IGF-1-induced lipogenesis and inflammation by targeting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways.

Key words: Paeoniflorin, Acne vulgaris, Sebocytes, Insulin-like growth factor-1

Chuanchuan Cai, Si Liu, Yufeng Liu, Shaobin Huang, Shiya Lu, Fang Liu, Xiaohua Luo, Christos C. Zouboulis, Ge Shi. Paeoniflorin mitigates insulin-like growth factor 1-induced lipogenesis and inflammation in human sebocytes by inhibiting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways[J]. 应用天然产物, 2024, 14(6): 56-56.

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Paeoniflorin mitigates insulin-like growth factor 1-induced lipogenesis and inflammation in human sebocytes by inhibiting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways

Paeoniflorin mitigates insulin-like growth factor 1-induced lipogenesis and inflammation in human sebocytes by inhibiting the PI3K/Akt/FoxO1 and JAK2/STAT3 signaling pathways

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