Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A

doi:10.1007/s13659-024-00471-x

应用天然产物 ›› 2024, Vol. 14 ›› Issue (6): 50-50.DOI: 10.1007/s13659-024-00471-x

Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A

Ya-Ya Yang¹, Shun-Li Jing¹, Jia-Li Shao¹, Ji-Xuan Chen¹, Wei-Feng Zhang¹, Si-Yuan Wan², Yu-Ping Shen¹, Huan Yang¹, Wei Yu²

1. School of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu Province, People's Republic of China;
2. Development Department, Jiangsu Grand Xianle Pharmaceutical Co., Ltd, Yancheng, 224555 People's Republic of China

收稿日期:2024-06-21 出版日期:2024-12-24 发布日期:2024-12-13
通讯作者: Huan Yang,E-mail:yanghuan1980@ujs.edu.cn;Wei Yu,E-mail:13784052172@163.com
基金资助:
This work was supported by the National Natural Science Foundation of China (Grant No: 81873196).

Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A

Ya-Ya Yang¹, Shun-Li Jing¹, Jia-Li Shao¹, Ji-Xuan Chen¹, Wei-Feng Zhang¹, Si-Yuan Wan², Yu-Ping Shen¹, Huan Yang¹, Wei Yu²

1. School of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu Province, People's Republic of China;
2. Development Department, Jiangsu Grand Xianle Pharmaceutical Co., Ltd, Yancheng, 224555 People's Republic of China

Received:2024-06-21 Online:2024-12-24 Published:2024-12-13
Contact: Huan Yang,E-mail:yanghuan1980@ujs.edu.cn;Wei Yu,E-mail:13784052172@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (Grant No: 81873196).

摘要/Abstract

摘要： Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl-γ-aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant β-glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products.

关键词: Immobilized β-glucosidase, Modified SBA-15, Sagittatoside A

Abstract: Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl-γ-aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant β-glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products.

Key words: Immobilized β-glucosidase, Modified SBA-15, Sagittatoside A

Ya-Ya Yang, Shun-Li Jing, Jia-Li Shao, Ji-Xuan Chen, Wei-Feng Zhang, Si-Yuan Wan, Yu-Ping Shen, Huan Yang, Wei Yu. Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A[J]. 应用天然产物, 2024, 14(6): 50-50.

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Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A

Purification and immobilization of β-glucosidase using surface modified mesoporous silica Santa Barbara Amorphous 15 for eco-friendly preparation of sagittatoside A

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