Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway

doi:10.1007/s13659-024-00458-8

应用天然产物 ›› 2024, Vol. 14 ›› Issue (5): 36-36.DOI: 10.1007/s13659-024-00458-8

• ORIGINAL ARTICLES • 上一篇下一篇

Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway

Yuru Shi, Xiaoqian Zhang, Shengji Pei, Yuhua Wang

Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Heilongtan, Kunming, 650201, Yunnan, China

收稿日期:2024-03-27 出版日期:2024-10-24 发布日期:2024-10-14
通讯作者: Yuhua Wang,E-mail:wangyuhua@mail.kib.ac.cn
基金资助:
This work was funded by Plant Germplasm Resources Innovation Project of Chinese Academy of Sciences (No. KFJ-BRP-007-002) and Yunnan Revitalization Talent Support Program Industrial Innovation Talent Project (Fleagrass Planting and Mosquito Repellent Product Industrialization Research and Development).

Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway

Yuru Shi, Xiaoqian Zhang, Shengji Pei, Yuhua Wang

Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Heilongtan, Kunming, 650201, Yunnan, China

Received:2024-03-27 Online:2024-10-24 Published:2024-10-14
Contact: Yuhua Wang,E-mail:wangyuhua@mail.kib.ac.cn
Supported by:
This work was funded by Plant Germplasm Resources Innovation Project of Chinese Academy of Sciences (No. KFJ-BRP-007-002) and Yunnan Revitalization Talent Support Program Industrial Innovation Talent Project (Fleagrass Planting and Mosquito Repellent Product Industrialization Research and Development).

摘要/Abstract

摘要： Adenosma buchneroides Bonati, also known as fleagrass, is an important medicinal plant used by the Akha (Hani) people of China for treating inflammation-related skin swelling, acne, and diarrhoea, among other conditions. In this study, we aimed to evaluate the anti-inflammatory activities and explore the molecular mechanisms of fleagrass on treating skin swelling and acne. The results demonstrated that fleagrass inhibited the enzymatic activities of 5-LOX and COX-2 in vitro, and decreased the release of NO, IL-6, TNF-α, and IL-10 in the LPS-induced RAW264.7 macrophages. The levels of proteins associated with the nuclear factor-kappa B (NF-κB) pathway were examined by western blotting and immunofluorescence, demonstrating that fleagrass downregulated the expression of TLR4, MyD88, NF-κB/p65, and iNOS and blocked the nuclear translocation of NF-κB/p65. Furthermore, fleagrass exhibited acute anti-inflammatory activity in paw oedema models. The results confirm that fleagrass exhibits remarkable anti-inflammatory activity and can be used in alleviating inflammation, suggesting that fleagrass has the potential to be a novel anti-inflammatory agent.

关键词: Fleagrass, Anti-inflammatory activities, TLR4/MyD88/NF-κB signaling pathway, Ethnopharmacology

Abstract: Adenosma buchneroides Bonati, also known as fleagrass, is an important medicinal plant used by the Akha (Hani) people of China for treating inflammation-related skin swelling, acne, and diarrhoea, among other conditions. In this study, we aimed to evaluate the anti-inflammatory activities and explore the molecular mechanisms of fleagrass on treating skin swelling and acne. The results demonstrated that fleagrass inhibited the enzymatic activities of 5-LOX and COX-2 in vitro, and decreased the release of NO, IL-6, TNF-α, and IL-10 in the LPS-induced RAW264.7 macrophages. The levels of proteins associated with the nuclear factor-kappa B (NF-κB) pathway were examined by western blotting and immunofluorescence, demonstrating that fleagrass downregulated the expression of TLR4, MyD88, NF-κB/p65, and iNOS and blocked the nuclear translocation of NF-κB/p65. Furthermore, fleagrass exhibited acute anti-inflammatory activity in paw oedema models. The results confirm that fleagrass exhibits remarkable anti-inflammatory activity and can be used in alleviating inflammation, suggesting that fleagrass has the potential to be a novel anti-inflammatory agent.

Key words: Fleagrass, Anti-inflammatory activities, TLR4/MyD88/NF-κB signaling pathway, Ethnopharmacology

Yuru Shi, Xiaoqian Zhang, Shengji Pei, Yuhua Wang. Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway[J]. 应用天然产物, 2024, 14(5): 36-36.

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Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway

Ethnopharmacological study on Adenosma buchneroides Bonati inhibiting inflammation via the regulation of TLR4/MyD88/NF-κB signaling pathway

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