Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation for the management of insulin resistance: chemical analysis and bioactivity

doi:10.1007/s13659-022-00352-1

应用天然产物 ›› 2022, Vol. 12 ›› Issue (5): 30-30.DOI: 10.1007/s13659-022-00352-1

Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation for the management of insulin resistance: chemical analysis and bioactivity

Ganna Kravchenko¹, Oksana Krasilnikova¹, Ain Raal², Matar Mazen¹, Natalia Chaika¹, Igor Kireyev¹, Andriy Grytsyk³, Oleh Koshovyi¹

1 National University of Pharmacy, 53 Pushkinska Str., Kharkiv 61002, Ukraine;
2 Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia;
3 Ivano-Frankivsk National Medical University, 2 Halytska Str., Ivano-Frankivsk 76018, Ukraine

收稿日期:2022-04-11 修回日期:2022-06-12 出版日期:2022-10-24 发布日期:2022-10-12
通讯作者: Ain Raal,E-mail:ain.raal@ut.ee
基金资助:
The research was funded by the Ministry of Health Care of Ukraine at the expense of the State Budget in the framework#2301020"Scientific and scientific-technical activity in the field of health protection "on the topic" Modern approaches to the creation of new medicines for a correction of metabolic syndrome".

Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation for the management of insulin resistance: chemical analysis and bioactivity

Ganna Kravchenko¹, Oksana Krasilnikova¹, Ain Raal², Matar Mazen¹, Natalia Chaika¹, Igor Kireyev¹, Andriy Grytsyk³, Oleh Koshovyi¹

1 National University of Pharmacy, 53 Pushkinska Str., Kharkiv 61002, Ukraine;
2 Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia;
3 Ivano-Frankivsk National Medical University, 2 Halytska Str., Ivano-Frankivsk 76018, Ukraine

Received:2022-04-11 Revised:2022-06-12 Online:2022-10-24 Published:2022-10-12
Contact: Ain Raal,E-mail:ain.raal@ut.ee
Supported by:
The research was funded by the Ministry of Health Care of Ukraine at the expense of the State Budget in the framework#2301020"Scientific and scientific-technical activity in the field of health protection "on the topic" Modern approaches to the creation of new medicines for a correction of metabolic syndrome".

摘要/Abstract

摘要： Bearberry (Arctostaphylos uva-ursi L.) is a perennial plant of the heather family (Ericaceae). The leaves are dominated by arbutin, phenol carbonic acids flavonoids, saponins, etc. It was previously shown that the bearberry leaves extract reduced blood glucose level in healthy animals under glucose overload, so it need to be studied more detail. The aim of the study was to investigate the chemical composition and the effect of dry alcohol extract from bearberry leaves, which enriched with cysteine, on the rats pancreas under experimental dexamethasone-induced insulin resistance (IR). Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation were obtained according to the developed method with 50% ethanol solution. Their phytochemical profile, hypoglycaemic and pancreatic protective effect were investigated. Phenologlycoside (arbutin), phenolic carboxylic acid (gallic acid), 5 flavonoids and 4 hydroxycinnamic acids were identified and quantified in the extracts by HPLC. Present data revealed that bearberry leaves alcoholic dry extract enriched with cysteine has a hypoglycaemic and pancreatic protective effect in treated animals under dexamethasone-induced IR model. Treatment improved hyperglycaemia, insulin resistance and beta cell reduction induced by dexamethasone injections.

关键词: Arctostaphylos uva-ursi L., Extract, Hypoglycaemic activity, Leaves, Phenolic substances

Abstract: Bearberry (Arctostaphylos uva-ursi L.) is a perennial plant of the heather family (Ericaceae). The leaves are dominated by arbutin, phenol carbonic acids flavonoids, saponins, etc. It was previously shown that the bearberry leaves extract reduced blood glucose level in healthy animals under glucose overload, so it need to be studied more detail. The aim of the study was to investigate the chemical composition and the effect of dry alcohol extract from bearberry leaves, which enriched with cysteine, on the rats pancreas under experimental dexamethasone-induced insulin resistance (IR). Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation were obtained according to the developed method with 50% ethanol solution. Their phytochemical profile, hypoglycaemic and pancreatic protective effect were investigated. Phenologlycoside (arbutin), phenolic carboxylic acid (gallic acid), 5 flavonoids and 4 hydroxycinnamic acids were identified and quantified in the extracts by HPLC. Present data revealed that bearberry leaves alcoholic dry extract enriched with cysteine has a hypoglycaemic and pancreatic protective effect in treated animals under dexamethasone-induced IR model. Treatment improved hyperglycaemia, insulin resistance and beta cell reduction induced by dexamethasone injections.

Key words: Arctostaphylos uva-ursi L., Extract, Hypoglycaemic activity, Leaves, Phenolic substances

Ganna Kravchenko, Oksana Krasilnikova, Ain Raal, Matar Mazen, Natalia Chaika, Igor Kireyev, Andriy Grytsyk, Oleh Koshovyi. Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation for the management of insulin resistance: chemical analysis and bioactivity[J]. 应用天然产物, 2022, 12(5): 30-30.

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Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation for the management of insulin resistance: chemical analysis and bioactivity

Arctostaphylos uva-ursi L. leaves extract and its modified cysteine preparation for the management of insulin resistance: chemical analysis and bioactivity

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