Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

doi:10.1007/s13659-020-00252-2

应用天然产物 ›› 2020, Vol. 10 ›› Issue (4): 227-241.DOI: 10.1007/s13659-020-00252-2

Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

Meilian Yang¹, Yudan Wang^1,2, Zhifeng Fan¹, Qingwang Xue³, Guy Sedar Singor Njateng⁴, Yaping Liu^1,4, Jianxin Cao¹, Tianrui Zhao¹, Guiguang Cheng¹

1 Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, People's Republic of China;
2 Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan Minzu University, Kunming 650500, People's Republic of China;
3 Department of Chemistry, Liaocheng University, Liaocheng 252059, Shandong, China;
4 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China

收稿日期:2020-05-13 修回日期:2020-06-04 出版日期:2020-08-24 发布日期:2020-08-19
通讯作者: Tianrui Zhao, Guiguang Cheng
基金资助:
This research was supported by the open program of State Key of Phytochemistry and Plant Resources in West China (P2018-KF08), the Applied Basic Research Project of Yunnan Province (Grant numbers No. 2018FB036 and 2017FD121), the National Natural Science Foundation of China (Grant Numbers 31600274).

Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

Meilian Yang¹, Yudan Wang^1,2, Zhifeng Fan¹, Qingwang Xue³, Guy Sedar Singor Njateng⁴, Yaping Liu^1,4, Jianxin Cao¹, Tianrui Zhao¹, Guiguang Cheng¹

1 Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, People's Republic of China;
2 Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan Minzu University, Kunming 650500, People's Republic of China;
3 Department of Chemistry, Liaocheng University, Liaocheng 252059, Shandong, China;
4 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China

Received:2020-05-13 Revised:2020-06-04 Online:2020-08-24 Published:2020-08-19
Contact: Tianrui Zhao, Guiguang Cheng
Supported by:
This research was supported by the open program of State Key of Phytochemistry and Plant Resources in West China (P2018-KF08), the Applied Basic Research Project of Yunnan Province (Grant numbers No. 2018FB036 and 2017FD121), the National Natural Science Foundation of China (Grant Numbers 31600274).

摘要/Abstract

摘要： Melodinus henryi is a good source of terpenoid indole alkaloids, and traditionally used as a folk medicine in the treatment of meningitis and fracture. In order to further exploit their potential uses, its anti-inflammatory and immunosuppressive activities, safety evaluations and chemical profiles have been illustrated. Compared to the crude methanol extract from M. henryi and its non-alkaloidal fraction, the total alkaloidal fraction (MHTA) had the strongest anti-inflammatory and immunosup-pressive activities. In the acute oral toxicity assay, the half lethal dose (LD₅₀) of MHTA was more than 2000 mg/kg. The sub-acute toxicity assay for consecutive 28 days exhibited MHTA at a lower concentrations of less than 500 mg/kg might be regarded as safe, and might damage spleen, liver, kidney, and heart when the dose is higher than 1000 mg/kg. In addition, a phytochemical investigation on MHTA led to the isolation of 15 monoterpenoid indole alkaloids. Thus, in regard with the potent side effects of MHTA, it should be used with caution in the development of phytomedicine.

关键词: Melodinus henryi, Acute toxicity, Subacute toxicity, Monoterpenoid indole alkaloids

Abstract: Melodinus henryi is a good source of terpenoid indole alkaloids, and traditionally used as a folk medicine in the treatment of meningitis and fracture. In order to further exploit their potential uses, its anti-inflammatory and immunosuppressive activities, safety evaluations and chemical profiles have been illustrated. Compared to the crude methanol extract from M. henryi and its non-alkaloidal fraction, the total alkaloidal fraction (MHTA) had the strongest anti-inflammatory and immunosup-pressive activities. In the acute oral toxicity assay, the half lethal dose (LD₅₀) of MHTA was more than 2000 mg/kg. The sub-acute toxicity assay for consecutive 28 days exhibited MHTA at a lower concentrations of less than 500 mg/kg might be regarded as safe, and might damage spleen, liver, kidney, and heart when the dose is higher than 1000 mg/kg. In addition, a phytochemical investigation on MHTA led to the isolation of 15 monoterpenoid indole alkaloids. Thus, in regard with the potent side effects of MHTA, it should be used with caution in the development of phytomedicine.

Key words: Melodinus henryi, Acute toxicity, Subacute toxicity, Monoterpenoid indole alkaloids

Meilian Yang, Yudan Wang, Zhifeng Fan, Qingwang Xue, Guy Sedar Singor Njateng, Yaping Liu, Jianxin Cao, Tianrui Zhao, Guiguang Cheng. Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents[J]. 应用天然产物, 2020, 10(4): 227-241.

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Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

Acute and Sub-Acute Toxicological Evaluations of Bioactive Alkaloidal Extract from Melodinus henryi and Their Main Chemical Constituents

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