UFLC-PDA-MS/MS Profiling of Seven Uncaria Species Integrated with Melatonin/5-Hydroxytryptamine Receptors Agonistic Assay

doi:10.1007/s13659-020-00230-8

应用天然产物 ›› 2020, Vol. 10 ›› Issue (1): 23-36.DOI: 10.1007/s13659-020-00230-8

• ORIGINAL ARTICLES • 上一篇下一篇

UFLC-PDA-MS/MS Profiling of Seven Uncaria Species Integrated with Melatonin/5-Hydroxytryptamine Receptors Agonistic Assay

Jian-Gang Zhang¹, Xiao-Yan Huang¹, Yun-Bao Ma¹, Ji-Jun Chen^1,2, Chang-An Geng¹

1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory of Natural Medicinal Chemistry, 132#Lanhei Road, Kunming 650201, Yunnan, People's Republic of China;
2 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China

收稿日期:2019-12-05 修回日期:2020-01-06 出版日期:2020-02-24 发布日期:2020-02-29
通讯作者: Chang-An Geng
基金资助:
This work was financially supported by the National Natural Science Foundation of China (81573322), the Yunnan Wanren Project (YNWR-QNBJ-2018-061), the Youth Innovation Promotion Association, CAS (2013252), the Program of Yunling Scholarship, the Yunnan Science Fund for Excellent Young Scholars (2019FI017), and the Reserve Talents of Young and Middle-aged Academic and Technical Leaders in Yunnan Province.

UFLC-PDA-MS/MS Profiling of Seven Uncaria Species Integrated with Melatonin/5-Hydroxytryptamine Receptors Agonistic Assay

Jian-Gang Zhang¹, Xiao-Yan Huang¹, Yun-Bao Ma¹, Ji-Jun Chen^1,2, Chang-An Geng¹

1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory of Natural Medicinal Chemistry, 132#Lanhei Road, Kunming 650201, Yunnan, People's Republic of China;
2 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China

Received:2019-12-05 Revised:2020-01-06 Online:2020-02-24 Published:2020-02-29
Contact: Chang-An Geng
Supported by:
This work was financially supported by the National Natural Science Foundation of China (81573322), the Yunnan Wanren Project (YNWR-QNBJ-2018-061), the Youth Innovation Promotion Association, CAS (2013252), the Program of Yunling Scholarship, the Yunnan Science Fund for Excellent Young Scholars (2019FI017), and the Reserve Talents of Young and Middle-aged Academic and Technical Leaders in Yunnan Province.

摘要/Abstract

摘要： Uncariae Ramulus Cum Uncis (Gou-Teng), the dried hook-bearing stems of several Uncaria plants (Rubiaceae), is a wellknown herbal medicine in China. The clinical application of Gou-Teng is bewildered for the morphological and chemical similarity between different species. In order to discern their chemical and biological difference, an ultra-fast liquid chromatography equipped with ion trap time-of-flight mass spectrometry (UFLC-IT/TOF-MS) combining with melatonin (MT₁ and MT₂) and 5-hydroxytryptamine (5-HT_1A and 5-HT_2C) receptors agonistic assay in vitro was conducted on seven Uncaria species. As a result, 57 compounds including 35 indole alkaloids, ten flavonoids, five triterpenoids, five chlorogenic analogues, and two other compounds were characterized based on their MS/MS patterns and UV absorptions. Specifically, cadambine-type and corynanthein-type alkaloids were exclusively present in U. rhynchophylla and U. scandens, whereas corynoxine-type alkaloids were commonly detected in all the seven Uncaria plants. Three Uncaria species, U. rhynchophylla, U. macrophylla, and U. yunnanensis showed obviously agnostic activity on four neurotransmitter receptors (MT₁, MT₂, 5-HT_1A, and 5-HT_2C). This first-time UFLCMS-IT-TOF analyses integrated with biological assay on seven Uncaria plants will provide scientific viewpoints for the clinical application of Gou-Teng.

关键词: Uncariae Ramulus Cum Uncis, Uncaria plants, LCMS-IT-TOF analyses, Melatonin and 5-hydroxytryptamine receptors

Abstract: Uncariae Ramulus Cum Uncis (Gou-Teng), the dried hook-bearing stems of several Uncaria plants (Rubiaceae), is a wellknown herbal medicine in China. The clinical application of Gou-Teng is bewildered for the morphological and chemical similarity between different species. In order to discern their chemical and biological difference, an ultra-fast liquid chromatography equipped with ion trap time-of-flight mass spectrometry (UFLC-IT/TOF-MS) combining with melatonin (MT₁ and MT₂) and 5-hydroxytryptamine (5-HT_1A and 5-HT_2C) receptors agonistic assay in vitro was conducted on seven Uncaria species. As a result, 57 compounds including 35 indole alkaloids, ten flavonoids, five triterpenoids, five chlorogenic analogues, and two other compounds were characterized based on their MS/MS patterns and UV absorptions. Specifically, cadambine-type and corynanthein-type alkaloids were exclusively present in U. rhynchophylla and U. scandens, whereas corynoxine-type alkaloids were commonly detected in all the seven Uncaria plants. Three Uncaria species, U. rhynchophylla, U. macrophylla, and U. yunnanensis showed obviously agnostic activity on four neurotransmitter receptors (MT₁, MT₂, 5-HT_1A, and 5-HT_2C). This first-time UFLCMS-IT-TOF analyses integrated with biological assay on seven Uncaria plants will provide scientific viewpoints for the clinical application of Gou-Teng.

Key words: Uncariae Ramulus Cum Uncis, Uncaria plants, LCMS-IT-TOF analyses, Melatonin and 5-hydroxytryptamine receptors

Jian-Gang Zhang, Xiao-Yan Huang, Yun-Bao Ma, Ji-Jun Chen, Chang-An Geng. UFLC-PDA-MS/MS Profiling of Seven Uncaria Species Integrated with Melatonin/5-Hydroxytryptamine Receptors Agonistic Assay[J]. 应用天然产物, 2020, 10(1): 23-36.

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UFLC-PDA-MS/MS Profiling of Seven Uncaria Species Integrated with Melatonin/5-Hydroxytryptamine Receptors Agonistic Assay

UFLC-PDA-MS/MS Profiling of Seven Uncaria Species Integrated with Melatonin/5-Hydroxytryptamine Receptors Agonistic Assay

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