Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives

doi:10.1007/s13659-018-0163-0

应用天然产物 ›› 2018, Vol. 8 ›› Issue (3): 189-197.DOI: 10.1007/s13659-018-0163-0

• ORIGINAL ARTICLE • 上一篇

Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives

Dong-Qiong Yang^1,2, Zhao-Rong Chen³, Duo-Zhi Chen¹, Xiao-Jiang Hao¹, Shun-Lin Li¹

1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China;
2 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China;
3 Tianjin Agricultural University, Tianjin 300380, People's Republic of China

收稿日期:2018-02-02 修回日期:2018-04-11 出版日期:2018-06-24 发布日期:2018-06-06
通讯作者: Shun-Lin Li
基金资助:
This work was financially supported by grants from the National Natural Science. Foundation of China (Nos. 31770389, 31470427, 31270404).

Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives

Dong-Qiong Yang^1,2, Zhao-Rong Chen³, Duo-Zhi Chen¹, Xiao-Jiang Hao¹, Shun-Lin Li¹

1 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China;
2 University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China;
3 Tianjin Agricultural University, Tianjin 300380, People's Republic of China

Received:2018-02-02 Revised:2018-04-11 Online:2018-06-24 Published:2018-06-06
Contact: Shun-Lin Li
Supported by:
This work was financially supported by grants from the National Natural Science. Foundation of China (Nos. 31770389, 31470427, 31270404).

摘要/Abstract

摘要： Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional halfleaf method. Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by westernblot and RT-PCR analysis. The tested compounds showed moderate inactivation effect, whereas the lycoricidine derivatives showed good protective effect. The protective inhibitory activity of compounds L1 (N-methyl-2,3,4-trimethoxylycoricidine) (60.8%) and L3 (N-methyl-2-methoxy-3,4-acetonidelycoricidine) (62.0%) was almost similar to the positive control, Ningnanmycin (66.4%). RT-PCR and Western-blot analysis displayed that compounds L1, L3, L5 (N-allyl-2,3,4-triallyloxylycoricidine) exhibited antiviral activity, which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression. Additionally, defensive enzyme activities confirmed that compound L1 could increase the activity of PAL, POD, SOD to improve disease resistance of tobacco.

关键词: Amaryllidaceae alkaloids, Lycoricidine derivatives, Lycoris radiata, Tobacco mosaic virus Anti-TMV activity

Abstract: Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional halfleaf method. Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by westernblot and RT-PCR analysis. The tested compounds showed moderate inactivation effect, whereas the lycoricidine derivatives showed good protective effect. The protective inhibitory activity of compounds L1 (N-methyl-2,3,4-trimethoxylycoricidine) (60.8%) and L3 (N-methyl-2-methoxy-3,4-acetonidelycoricidine) (62.0%) was almost similar to the positive control, Ningnanmycin (66.4%). RT-PCR and Western-blot analysis displayed that compounds L1, L3, L5 (N-allyl-2,3,4-triallyloxylycoricidine) exhibited antiviral activity, which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression. Additionally, defensive enzyme activities confirmed that compound L1 could increase the activity of PAL, POD, SOD to improve disease resistance of tobacco.

Key words: Amaryllidaceae alkaloids, Lycoricidine derivatives, Lycoris radiata, Tobacco mosaic virus Anti-TMV activity

Dong-Qiong Yang, Zhao-Rong Chen, Duo-Zhi Chen, Xiao-Jiang Hao, Shun-Lin Li. Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives[J]. 应用天然产物, 2018, 8(3): 189-197.

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Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives

Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives

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