Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L.(Lamiaceae)

doi:10.1007/s13659-015-0070-6

应用天然产物 ›› 2015, Vol. 5 ›› Issue (4): 215-221.DOI: 10.1007/s13659-015-0070-6

• Original article • 上一篇

Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L.(Lamiaceae)

Dorothée Dinangayi Tshilanda¹, Damase NguwoVele Onyamboko¹, Philippe Babady-Bila^1,2, Koto-te-Nyiwa Ngbolua¹, Damien ShaTshibey Tshibangu¹, Eddy dia Fita Dibwe^1,3, Pius Tshimankinda Mpiana¹

1. Faculté des Sciences, Université de Kinshasa, B. P. 190, Kinshasa XI, Democratic Republic of the Congo;
2. Bila Nutraceuticals Company, 650 Portland Street Market, PO Box 26056, Dartmouth, NS B2 W6 P3, Canada;
3. Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama 930-0194, Japan

收稿日期:2015-07-13 修回日期:2015-08-25 出版日期:2015-08-24 发布日期:2018-02-11
通讯作者: Philippe Babady-Bila,e-mail:pbabady@bilanutraceuticals.com

Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L.(Lamiaceae)

Dorothée Dinangayi Tshilanda¹, Damase NguwoVele Onyamboko¹, Philippe Babady-Bila^1,2, Koto-te-Nyiwa Ngbolua¹, Damien ShaTshibey Tshibangu¹, Eddy dia Fita Dibwe^1,3, Pius Tshimankinda Mpiana¹

1. Faculté des Sciences, Université de Kinshasa, B. P. 190, Kinshasa XI, Democratic Republic of the Congo;
2. Bila Nutraceuticals Company, 650 Portland Street Market, PO Box 26056, Dartmouth, NS B2 W6 P3, Canada;
3. Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama 930-0194, Japan

Received:2015-07-13 Revised:2015-08-25 Online:2015-08-24 Published:2018-02-11

摘要/Abstract

摘要： The present study reports in vitro anti-sickling activity and phytochemical analyses of the leaves of Ocimum gratissimum. Biological testing revealed that the plant extracts possess antisickling effects. The combination of spectroscopic techniques:1D-NMR, 2D-NMR and MS revealed that ursolic acid is the major biologically active compound of O. gratissimum(Silva et al. in Molecules 13:2482-2487, 2008;Kedar et al. J Food Drug Anal 20:865-871, 2012). This study is the first report of the antisickling activity of ursolic acid isolated from O. gratissimum. The pharmaceutical relevance of findings from this study derives from the possibility of integrating O. gratissimum as an antisickling plant in the pharmacopoeia of Democratic Republic of the Congo. The identification of the active principle could enhance the standardization of antisickling recipe.

关键词: Ocimum gratissimum, Sickle cell disease, Antisickling activity, Ursolic acid

Abstract: The present study reports in vitro anti-sickling activity and phytochemical analyses of the leaves of Ocimum gratissimum. Biological testing revealed that the plant extracts possess antisickling effects. The combination of spectroscopic techniques:1D-NMR, 2D-NMR and MS revealed that ursolic acid is the major biologically active compound of O. gratissimum(Silva et al. in Molecules 13:2482-2487, 2008;Kedar et al. J Food Drug Anal 20:865-871, 2012). This study is the first report of the antisickling activity of ursolic acid isolated from O. gratissimum. The pharmaceutical relevance of findings from this study derives from the possibility of integrating O. gratissimum as an antisickling plant in the pharmacopoeia of Democratic Republic of the Congo. The identification of the active principle could enhance the standardization of antisickling recipe.

Key words: Ocimum gratissimum, Sickle cell disease, Antisickling activity, Ursolic acid

Dorothée Dinangayi Tshilanda, Damase NguwoVele Onyamboko, Philippe Babady-Bila, Koto-te-Nyiwa Ngbolua, Damien ShaTshibey Tshibangu, Eddy dia Fita Dibwe, Pius Tshimankinda Mpiana. Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L.(Lamiaceae)[J]. 应用天然产物, 2015, 5(4): 215-221.

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Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L.(Lamiaceae)

Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L.(Lamiaceae)

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