A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes

doi:10.1007/s13659-015-0063-5

应用天然产物 ›› 2015, Vol. 5 ›› Issue (3): 159-166.DOI: 10.1007/s13659-015-0063-5

A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes

Ji-Huan Qin¹, Jun-Zeng Ma², Xing-Wei Yang², Ying-Jie Hu¹, Juan Zhou¹, Lin-Chun Fu¹, Ru-Hua Tian¹, Shan Liu¹, Gang Xu², Xiao-Ling Shen¹

1. Laboratory of Chinese Herbal Drug Discovery, Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, People's Republic of China;
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China

收稿日期:2015-04-15 修回日期:2015-05-25 出版日期:2015-06-24 发布日期:2018-02-11
基金资助:
This work was financially supported by China National Major Projects of Science&Technology(2014ZX10005002; 2009ZX09103-436), the Young Academic Leader Raising Foundation of Yunnan Province(No. 2009CI073), the foundation from Chinese Academy of Sciences to Gang Xu, and the Program for Research Team in South China Chinese Medicine Collaborative Innovation Center of Guangdong, China(A1-AFD01514A07).

A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes

Ji-Huan Qin¹, Jun-Zeng Ma², Xing-Wei Yang², Ying-Jie Hu¹, Juan Zhou¹, Lin-Chun Fu¹, Ru-Hua Tian¹, Shan Liu¹, Gang Xu², Xiao-Ling Shen¹

1. Laboratory of Chinese Herbal Drug Discovery, Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, People's Republic of China;
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, People's Republic of China

Received:2015-04-15 Revised:2015-05-25 Online:2015-06-24 Published:2018-02-11
Supported by:
This work was financially supported by China National Major Projects of Science&Technology(2014ZX10005002; 2009ZX09103-436), the Young Academic Leader Raising Foundation of Yunnan Province(No. 2009CI073), the foundation from Chinese Academy of Sciences to Gang Xu, and the Program for Research Team in South China Chinese Medicine Collaborative Innovation Center of Guangdong, China(A1-AFD01514A07).

摘要/Abstract

摘要： 6α-Hydroxylup-20(29)-en-3-on-28-oic acid(1), a natural triterpenoid, was found to possess the ability in a dose-dependent manner inhibiting hormone-induced adipocyte differentiation in 3T3-L1 preadipocytes, and restoring glucose consuming ability in dexamethasone(DXM)-induced insulin resistant 3T3-L1 adipocytes. Compound 1 was also found to ameliorate DXM-induced adipocyte dysfunction in lipolysis and adipokine secretion. Mechanistic studies revealed that 1 inhibited adipocyte differentiation in 3T3-L1 preadipocytes via down-regulating hormone-stimulated gene transcription of peroxisome proliferator-activated receptor γ and CCAAT-enhancer-binding protein alpha which are key factors in lipogenesis, and restored DXM-impaired glucose consuming ability in differentiated 3T3-L1 adipocytes via repairing insulin signaling pathway and activating down-stream signaling transduction by phosphorylation of signaling molecules PI3K/p85, Akt2 and AS160, thus leading to increased translocation of glucose transporter type 4 and transportation of glucose.

关键词: 6α-Hydroxylup-20(29)-en-3-on-28-oic acid, 3T3-L1, Adipocyte differentiation, Dexamethasoneinduced insulin resistance, Adipocyte dysfunction, PI3K/Akt2 signaling

Abstract: 6α-Hydroxylup-20(29)-en-3-on-28-oic acid(1), a natural triterpenoid, was found to possess the ability in a dose-dependent manner inhibiting hormone-induced adipocyte differentiation in 3T3-L1 preadipocytes, and restoring glucose consuming ability in dexamethasone(DXM)-induced insulin resistant 3T3-L1 adipocytes. Compound 1 was also found to ameliorate DXM-induced adipocyte dysfunction in lipolysis and adipokine secretion. Mechanistic studies revealed that 1 inhibited adipocyte differentiation in 3T3-L1 preadipocytes via down-regulating hormone-stimulated gene transcription of peroxisome proliferator-activated receptor γ and CCAAT-enhancer-binding protein alpha which are key factors in lipogenesis, and restored DXM-impaired glucose consuming ability in differentiated 3T3-L1 adipocytes via repairing insulin signaling pathway and activating down-stream signaling transduction by phosphorylation of signaling molecules PI3K/p85, Akt2 and AS160, thus leading to increased translocation of glucose transporter type 4 and transportation of glucose.

Key words: 6α-Hydroxylup-20(29)-en-3-on-28-oic acid, 3T3-L1, Adipocyte differentiation, Dexamethasoneinduced insulin resistance, Adipocyte dysfunction, PI3K/Akt2 signaling

Ji-Huan Qin, Jun-Zeng Ma, Xing-Wei Yang, Ying-Jie Hu, Juan Zhou, Lin-Chun Fu, Ru-Hua Tian, Shan Liu, Gang Xu, Xiao-Ling Shen. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes[J]. 应用天然产物, 2015, 5(3): 159-166.

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A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes

A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes

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