A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog

doi:10.1007/s13659-014-0037-z

应用天然产物 ›› 2014, Vol. 4 ›› Issue (5): 309-313.DOI: 10.1007/s13659-014-0037-z

• Original article • 上一篇

A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog

Guo-Xiang Mo¹, Xue-Wei Bai¹, Zong-Jie Li¹, Xiu-Wen Yan¹, Xiao-Qing He², Ming-Qiang Rong²

1. School of Biological Sciences, Nanjing Agriculture University, Nanjing 210095, Jiangshu, China;
2. Kunming Institue of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China

收稿日期:2014-06-30 修回日期:2014-08-21 出版日期:2014-10-24 发布日期:2018-02-11
通讯作者: Ming-Qiang Rong,e-mail:rongmingqiang@mail.kiz.ac.cn
基金资助:
This work was supported by National Natural Science Foundation(31200590), Jiangsu Province(BK2012365, BE2012748) and Yunan Province(2013FB072).

A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog

Guo-Xiang Mo¹, Xue-Wei Bai¹, Zong-Jie Li¹, Xiu-Wen Yan¹, Xiao-Qing He², Ming-Qiang Rong²

1. School of Biological Sciences, Nanjing Agriculture University, Nanjing 210095, Jiangshu, China;
2. Kunming Institue of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China

Received:2014-06-30 Revised:2014-08-21 Online:2014-10-24 Published:2018-02-11
Supported by:
This work was supported by National Natural Science Foundation(31200590), Jiangsu Province(BK2012365, BE2012748) and Yunan Province(2013FB072).

摘要/Abstract

摘要： Various kinds of biologically active peptides have previously been isolated from the skin secretions of Amolops loloensis frog, such as antimicrobial peptides, bradykinin-like peptides and algesic peptides. A novel insulinotropic peptide named amolopin was identified in A. loloensis frog's skin secretion. Its primary structure sequence was determined by Edman degradation as: FLPIVGKSLSGLSGKL-NH2. BLAST search indicates that the amino acid sequence of amolopin is quite different from other known insulin secretagogues, including mastoparan, exendins and a-latrotoxin, nor does it like incretins(e.g. glucagons like peptide-1 and glucose-dependent insulinotropic ploypeptide) either. However, amolopin shows certain structural similarity with amphibian antimicrobial temporins and vespid chemotactic peptides isolated from Vespa magnifica. Amolopin can stimulate insulin release in INS-1 cells in a dose-dependent manner. Primary investigation on its action mechanisms reveals that amolopin does not increase the influx of Ca²⁺. In conclusion, a novel 16-amino acid peptide with insulin-releasing activity is initially discovered from the skin secretion of A. loloensis frog. Further work is necessary to evaluate its potential as novel anti-diabetic candidate.

关键词: Insulinotropic peptide, Amolops loloensis, Skin secretion, Insulin-releasing, Frog

Abstract: Various kinds of biologically active peptides have previously been isolated from the skin secretions of Amolops loloensis frog, such as antimicrobial peptides, bradykinin-like peptides and algesic peptides. A novel insulinotropic peptide named amolopin was identified in A. loloensis frog's skin secretion. Its primary structure sequence was determined by Edman degradation as: FLPIVGKSLSGLSGKL-NH2. BLAST search indicates that the amino acid sequence of amolopin is quite different from other known insulin secretagogues, including mastoparan, exendins and a-latrotoxin, nor does it like incretins(e.g. glucagons like peptide-1 and glucose-dependent insulinotropic ploypeptide) either. However, amolopin shows certain structural similarity with amphibian antimicrobial temporins and vespid chemotactic peptides isolated from Vespa magnifica. Amolopin can stimulate insulin release in INS-1 cells in a dose-dependent manner. Primary investigation on its action mechanisms reveals that amolopin does not increase the influx of Ca²⁺. In conclusion, a novel 16-amino acid peptide with insulin-releasing activity is initially discovered from the skin secretion of A. loloensis frog. Further work is necessary to evaluate its potential as novel anti-diabetic candidate.

Key words: Insulinotropic peptide, Amolops loloensis, Skin secretion, Insulin-releasing, Frog

Guo-Xiang Mo, Xue-Wei Bai, Zong-Jie Li, Xiu-Wen Yan, Xiao-Qing He, Ming-Qiang Rong. A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog[J]. 应用天然产物, 2014, 4(5): 309-313.

Guo-Xiang Mo, Xue-Wei Bai, Zong-Jie Li, Xiu-Wen Yan, Xiao-Qing He, Ming-Qiang Rong. A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog[J]. Natural Products and Bioprospecting, 2014, 4(5): 309-313.

参考文献

1. M. Simmaco, G. Mignogna, D. Barra, Biopolymers 47, 435-450 (1998)
2. J. Li, X. Xu, C. Xu, W. Zhou, K. Zhang, H. Yu, Y. Zhang, Y. Zheng, H.H. Rees, R. Lai, D. Yang, J. Wu, Mol. Cell. Proteomics 6, 882-894 (2007)
3. J.P. Raufman, Regul. Pept. 61, 1-18 (1996)
4. T.A. Nogueira, F. Ferreira, H.M. Toyama, F.L. Stoppiglia, S. Marangoni, A. Boschero, M.E. Carneiro, Toxicon 45, 243-248 (2005)
5. N. Yokokawa, M. Komatsu, T. Takeda, T. Aizawa, T. Yamada, Biochem. Biophys. Res. Commun. 158, 712-716 (1989)
6. A. Rohou, J. Nield, Y.A. Ushkaryov, Toxicon 49, 531-549 (2007)
7. C.A. Ashton, M.A. Rahman, E.K. Volynski, C. Manser, V.E. Orlova, H. Matsushita, A.B. Davletov, V.M. Heel, V.E. Grishin, A.Y. Ushkaryov, Biochimie 82, 453-468 (2000)
8. D.I. Israel, Nucleic Acids Res. 21, 2627-2631 (1993)
9. P.M. Jones, F.M. Mann, S.J. Persaud, C.P. Wheeler-Jones, Mol. Cell. Endocrinol. 94, 97-103 (1993)
10. H.R. Amin, H. Chen, R. Veluthakal, B.R. Silver, J. Li, A. Kowluru, Endocrinology 144, 4508-4518 (2003)
11. M. Simmaco, G. Mignogna, S. Canofeni, R. Miele, M.L. Mangoni, D. Barra, Eur. J. Biochem. 242, 788-792 (1996)
12. Y.H. Abdel-Wahab, L. Marenah, D.F. Orr, C. Shaw, P.R. Flatt, Biol. Chem. 386, 58-581 (2005)
13. L. Marenah, P.R. Flatt, D.F. Orr, S. McClean, C. Shaw, Y.H. Abdel-Wahab, J. Endocrinol. 181, 347-354 (2004)
14. L. Marenah, P.R. Flatt, D.F. Orr, C. Shaw, Y.H. Abdel-Wahab, Peptides 26, 2117-2123 (2005)
15. L. Marenah, P.R. Flatt, D.F. Orr, C. Shaw, Y.H. Abdel-Wahab, J. Endocrinol. 188, 1-9 (2006)
16. Y.H. Abdel-Wahab, L. Marenah, P.R. Flatt, J.M. Conlon, Protein Pept. Lett. 14, 702-707 (2007)
17. J. Liang, Y. Han, J. Li, X. Xu, H.H. Rees, R. Lai, Peptides 27, 2683-2687 (2006)
18. Y. Lu, J. Li, H. Yu, X. Xu, J. Liang, Y. Tian, D. Ma, G. Lin, G. Huang, R. Lai, Peptides 27, 3085-3091 (2006)
19. H. Yu, X. Wang, J. Liu, R. Lai, J. Pept. Sci. 13, 798-802 (2007)
20. M. Asfari, D. Janjic, P. Meda, G. Li, A.P. Halban, B.C. Wollheim, Endocrinology 130, 167-178 (1992)
21. E. Santini, P. Fallahi, M.S. Ferrari, A. Masoni, A. Antonelli, E. Ferranini, Diabetes 53, 79-83 (2004)
22. K.J. Mitchell, T. Tsuboi, G.A. Rutter, Diabetes 53, 393-400 (2004)
23. J. Fauconnier, D.C. Andersson, S.J. Zhang, J.T. Lanner, R. Wibom, A. Katz, J.D. Bruton, H. Westerblad, Diabetes 56, 1136-1142 (2007)
24. X. Hu, M. Jiang, J. Zhang, A. Zhang, F. Lin, M. Tan, New Phytol. 173, 27-38 (2007)
25. H. Feng, Y. Lv, M. Wang, J, Li. Zool. Res. 30, 165-170 (2009)
26. X. Xu, J. Li, Q. Lu, H. Yang, Y. Zhang, R. Lai, Toxicon 47, 249-253 (2006)
27. A. Dubois, Bull. Mens. Soc. Linn. Lyon. 61, 305-352 (1992)
28. C. Liu, Fieldiana Zool. Mem. 2, 1-400 (1950)
29. N. Morikawa, K. Hagiwara, T. Nakajima, Biochem. Biophys. Res. Commun. 189, 184-190 (1992)
30. M.L. Mangoni, J.M. Saugar, M. Dellisanti, D. Barra, M. Simmaco, L. Rivas, J. Biol. Chem. 280, 984-990 (2005)
31. M. Zhou, L. Wang, D.E. Owens, T. Chen, B. Walker, C. Shaw, Peptides 28, 1605-1610 (2007)
32. E.H. Hohmeier, B.C. Newgard, Mol. Cell. Endocrinol. 228, 121-128 (2004)

A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog

A Novel Insulinotropic Peptide from the Skin Secretions of Amolops loloensis Frog

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