整合生物学期刊网

应用天然产物 ›› 2014, Vol. 4 ›› Issue (3): 141-147.DOI: 10.1007/s13659-014-0017-3

• Original article • 上一篇    下一篇

Evaluation of Various Factors Affecting Bioconversion of L-Tyrosine to L-DOPA by Yeast Yarrowia lipolytica-NCIM 3450 Using Response Surface Methodology

Swati T. Gurme1, Shripad N. Surwase2, Sushama A. Patil1, Jyoti P. Jadhav1   

  1. 1. Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur 416004, India;
    2. Department of Microbiology, Shivaji University, Kolhapur 416004, India
  • 收稿日期:2014-01-27 修回日期:2014-01-27 出版日期:2014-06-24 发布日期:2018-02-11
  • 通讯作者: Jyoti P.Jadhav,e-mail:jpjbiochem@gmail.com
  • 基金资助:
    Swati T. Gurme thankful to the Department of Science and Technology, Government of India, for providing those fellowships under the DST-PURSE program. Dr. Shripad N. Surwase thanks the Lady Tata Memorial Trust, Mumbai, Maharashtra, India, for awarding him with a Junior Research Scholarship for his doctoral research. Sushama A. Patil thank to the Department of Science and Technology, Government of India, for providing those fellowships under the DST-SAP program. Authors thankful to Karen Dulski Communications Specialist Stat-Ease Inc. USA for extending the trial period of Design Expert software(version 8. 0, Stat-Ease Inc. USA). Corresponding author wishes to thank Interdisciplinary Programme for Life Sciences sponsored by Dept of Biotechnology Government of India under DBT-IPLS program.

Evaluation of Various Factors Affecting Bioconversion of L-Tyrosine to L-DOPA by Yeast Yarrowia lipolytica-NCIM 3450 Using Response Surface Methodology

Swati T. Gurme1, Shripad N. Surwase2, Sushama A. Patil1, Jyoti P. Jadhav1   

  1. 1. Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur 416004, India;
    2. Department of Microbiology, Shivaji University, Kolhapur 416004, India
  • Received:2014-01-27 Revised:2014-01-27 Online:2014-06-24 Published:2018-02-11
  • Supported by:
    Swati T. Gurme thankful to the Department of Science and Technology, Government of India, for providing those fellowships under the DST-PURSE program. Dr. Shripad N. Surwase thanks the Lady Tata Memorial Trust, Mumbai, Maharashtra, India, for awarding him with a Junior Research Scholarship for his doctoral research. Sushama A. Patil thank to the Department of Science and Technology, Government of India, for providing those fellowships under the DST-SAP program. Authors thankful to Karen Dulski Communications Specialist Stat-Ease Inc. USA for extending the trial period of Design Expert software(version 8. 0, Stat-Ease Inc. USA). Corresponding author wishes to thank Interdisciplinary Programme for Life Sciences sponsored by Dept of Biotechnology Government of India under DBT-IPLS program.

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摘要： 3, 4-Dihydroxy L-phenylalanine(L-DOPA) is considered a potent drug for the treatment of Parkinson disease. Physical and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the highest production of L-DOPA. Screenings of critical components were completed by using a Plackett-Burman design, while further optimization was carried out using the Box-Behnken design. The optimized factor levels predicted by the model were pH 6.1, 1.659 g L-1 yeast extract, 1.491 g L-1 L-tyrosine and 0.0290 g L-1 CuSO4. The predicted yield of L-DOPA with these levels was 1.319 g L-1, while actual yield obtained was 1.273 g L-1. The statistical analysis revealed that model is significant with F value 19.55 and R2 value 0.9514. This process resulted in a 3.594-fold increase in the yield of LDOPA. L-DOPA was confirmed by HPTLC and HPLC analysis. Thus, Yarrowia lipolytica-NCIM 3450 has potential to be a new source for the production of L-DOPA.

关键词: L-DOPA, L-tyrosine, RSM, Yarrowia lipolytica

Abstract: 3, 4-Dihydroxy L-phenylalanine(L-DOPA) is considered a potent drug for the treatment of Parkinson disease. Physical and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the highest production of L-DOPA. Screenings of critical components were completed by using a Plackett-Burman design, while further optimization was carried out using the Box-Behnken design. The optimized factor levels predicted by the model were pH 6.1, 1.659 g L-1 yeast extract, 1.491 g L-1 L-tyrosine and 0.0290 g L-1 CuSO4. The predicted yield of L-DOPA with these levels was 1.319 g L-1, while actual yield obtained was 1.273 g L-1. The statistical analysis revealed that model is significant with F value 19.55 and R2 value 0.9514. This process resulted in a 3.594-fold increase in the yield of LDOPA. L-DOPA was confirmed by HPTLC and HPLC analysis. Thus, Yarrowia lipolytica-NCIM 3450 has potential to be a new source for the production of L-DOPA.

Key words: L-DOPA, L-tyrosine, RSM, Yarrowia lipolytica

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