关键词: 非靶向代谢组学, 高浓度Ca²⁺, 代谢通路, 适应机, 苗药

Abstract: Drynaria roosii is a typical epiphytic pteridophyte in Karst area, which lives on the surface or crevice of rocks mainly through its rhizome. The dried rhizome of D. roosii is used as medicine and has important medicinal value. In order to study the changes of metabolites in D. roosii rhizome under Ca²⁺ stress, the D. roosii rhizome under different concentrations(0, 600, 1 200 mmol·L^-1)of Ca²⁺ stress was analyzed by using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF/MS). The results were as follows:(1)A total of 64 differentially expressed metabolites were identified.(2)Forty-eight differentially expressed metabolites were identified between the 0 mmol·L^-1and 600 mmol·L^-1 comparison group, 45 differentially expressed metabolites were identified between the 0 mmol·L^-1 and 1 200 mmol·L^-1 comparison group, and 44 differentially expressed metabolites were identified between the 600 and 1 200 mmol·L^-1 comparison group.(3)The identified differentially expressed metabolites were classified into five categories according to their chemical classification information. In summary, Ca²⁺ stress affects the amino acid metabolism, flavonoids biosynthesis, lignin biosynthesis, fatty acid metabolism and other pathways of D. roosii rhizome. Through non-targeted metabolomics analysis, the key metabolites that response to Ca²⁺ stress in D. roosii rhizome are revealed. These results lay a foundation for further study on the regulatory mechanism of D. roosii adaptation to Ca²⁺ stress, and also provide a new idea for the quality improvement of medicinal materials of D. roosii rhizome.

Key words: non-targeted metabolomics, high concentration of Ca²⁺, metabolic pathway, adaptive mechanism, Miao medicine