Proteomic Screening and RcBSK7 Resistance of Ricinus communis under Cadmium(Cd) Stress

doi:10.7525/j.issn.1673-5102.2023.01.005

Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (1): 36-50.doi: 10.7525/j.issn.1673-5102.2023.01.005

• Genetic and Breeding • Previous Articles Next Articles

Proteomic Screening and RcBSK7 Resistance of Ricinus communis under Cadmium(Cd) Stress

Huibo ZHAO¹, Zhiqiang ZHAO², Chunguang BAO³, Qi WEN¹, Ruxin LI¹, Fenglan HUANG⁴^,⁵^,⁶()

^1.College of Life Science and Food，Inner Mongolia University for Nationalities，Tongliao 028000
^2.College of Agriculture，Inner Mongolia University for Nationalities，Tongliao 028000
^3.Tongliao Agricultural and Livestock Product Quality and Safety Center，Tongliao 028000
^4.Castor-oil Plant Breeding，National Civil Affairs Commission Key Experiment Laboratory，Tongliao 028000
^5.Castor Industry Engineering Technology Research Center，Inner Mongolia Autonomous Region University，Tongliao 028000
^6.Castor Breeding Laboratory，Inner Mongolia Autonomous Region，Tongliao 028000

Received:2021-11-22 Online:2023-01-20 Published:2022-12-23
Contact: Fenglan HUANG E-mail:huangfenglan@imun.edu.cn
About author:ZHAO Huibo（1997—），famale，postgraduates，major in plant biochemistry and molecular biology.
Supported by:
National Natural Science Foundation of China(31860071);2020 New Agricultural Science Research and Reform Practice Project of the Ministry of Education(2020082);General Project of Inner Mongolia Natural Science Foundation(2021MS03008);Inner Mongolia Autonomous Region Grassland Talents Rolling Support Program Project;Castor Molecular Breeding Research Innovation Talent Team Rolling Support Plan;2021 National Ethnic Affairs Commission Higher Education Teaching Reform Research Project（21082）;Inner Mongolia Autonomous Region Castor Industry Collaborative Innovation Center Open Fund Project(MDK2021011)

Abstract

Abstract:

In order to reveal the mechanism of Ricinus communis response to heavy metal cadmium（Cd） stress， and screen out the resistance genes involved in Cd stress， by observing the seed germination and plant growth status， castor plants treated with water were used as controls to study their effects under three doses（300， 700 and 1 000 mg·L^-1） of Cd stress. The response mechanism is expected to provide new ideas for revealing the defense and detoxification mechanism of castor plant under in Cd stress. Using differential proteomics to analyze the network regulation mechanism of castor under Cd stress， that is， with the increase of Cd stress concentration， the castor plant blocks the absorption of heavy metal Cd by roots， improves their own antioxidant capacity， and inhibits Cd²⁺ operation. As well as the induction of programmed cell death and other detoxification processes to resist Cd stress damage； according to the results of omics analysis， a significantly different gene RcBSK7 was screened. Through functional verification in Arabidopsis， it can be seen that this gene can improve tolerance of castor plants to Cd stress. This study enhances the understanding of the diversity and complexity of castor plants under three Cd stresses， and provides a valuable theoretical basis for the identification of Cd-tolerant genes and the restoration of heavy metal pollution in the soil.

Key words: Ricinus communis, heavy metal Cd, differential proteomics, serine/threonine protein kinase, soil pollution remediation

CLC Number:

Q344+.13

Huibo ZHAO, Zhiqiang ZHAO, Chunguang BAO, Qi WEN, Ruxin LI, Fenglan HUANG. Proteomic Screening and RcBSK7 Resistance of Ricinus communis under Cadmium(Cd) Stress[J]. Bulletin of Botanical Research, 2023, 43(1): 36-50.

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Proteomic Screening and RcBSK7 Resistance of Ricinus communis under Cadmium(Cd) Stress

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