Rapid Micropropagation of Fraxinus mandshurica Rupr. Based on Liquid and Solid Alternate Culture

doi:10.7525/j.issn.1673-5102.2022.02.003

Abstract

Abstract:

A rapid and efficient regeneration system of liquid-solid alternate culture was developed for the tissue culture seedlings of Fraxinus mandshurica with lignificated stalk， aged leaves and dormant apical buds， and the axillary buds were germinated in a short time by liquid suspension culture， and the axillary buds were regenerated in vitro in solid culture to obtain the new tissue culture seedlings. On liquid and solid media supplemented of WPM with different plant growth regulators， axillary buds of F. mandshurica were induced to germinate and grow into seedlings. It was found that the axillary bud germination rate of F. mandshurica could reach 100% within 7-15 d after dark culture in WPM liquid medium supplemented with 0.6 mg·L^-1 TDZ. After cutting the sprouted buds and inoculating them into WPM solid medium of 0.05 mg·L^-1 TDZ and 0.6 mg·L^-1 BA， axillary buds could grow into shoots after 1-2 subcultures with an average height of 2.64 cm and a multiplication coefficient of 4.04. After transplanting， the survival rate of rooting seedlings was 90% after 50 d. The establishment of the method could be conducive to the large-scale propagation of F. mandshurica， and the liquid-solid alternate circulation culture， simple， controllable， easy to operate， suitable for different production conditions and reduces the cost.

Key words: Fraxinus mandshurica Rupr., dormant bud, tissue culture, rapid reproduction

CLC Number:

Q949.776.2

Fenghui Qi, Wenxuan Wang, Lin Liu, Mingshuo Tang, Feixiang Song, Yaguang Zhan. Rapid Micropropagation of Fraxinus mandshurica Rupr. Based on Liquid and Solid Alternate Culture[J]. Bulletin of Botanical Research, 2022, 42(2): 184-190.

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