Metabolic Differences Between Wild and Cultivated Species of Taxus cuspidata Based on GC-MS

doi:10.7525/j.issn.1673-5102.2021.05.018

Abstract

Abstract:

In order to explore the differences of metabolites between wild and cultivated Taxus cuspidata， nontargeted metabolomics was analyzed. Using GC-MS and multivariate statistical analysis to compare the bark of wild species and cultivated species， the differences in their metabolites were found. A total of 45 significant metabolites were detected， including 4 amino acids， 10 organic acids and some sugar alcohols. Among them， 22 metabolites were up-regulated and the 23 metabolites were down-regulated. KEGG pathway analysis showed that seven pathways had significant effects， including sulfur metabolism， arginine and proline metabolism， pentose phosphate pathway， unsaturated fatty acid biosynthesis， galactose metabolism， alanine， aspartate and glutamic acid metabolism， phenylpropanoid biosynthesis. The metabolites such as amino acids and organic acids were highly expressed in cultivated T. cuspidata. The stress resistance of wild T. cuspidata was weaker than that of cultivated T. cuspidata. However， the expression of carbohydrate was higher in wild species. T. cuspidata wild species had better glucose metabolism ability to resist low temperature stress. The results provided a theoretical basis for the protection of T. cuspidata wild species and stress resistance mechanism of T. cuspidata.

Key words: Taxus cuspidata, metabonomics, GC-MS

CLC Number:

S791.49

Long-Jie LIU, Ke-Xin WU, Yun-Fei DIAO, Zhong-Yue LI, Li-Qiang MU. Metabolic Differences Between Wild and Cultivated Species of Taxus cuspidata Based on GC-MS[J]. Bulletin of Botanical Research, 2021, 41(5): 798-806.

References

1	尹雪，穆立蔷，李中跃，等.3种鸟类对东北红豆杉的取食方式及传播［J］.东北林业大学学报，2016，44（1）：81-84，89.
1	Yin X，Mu L Q，Li Z Y，et al.Fruits foraging patterns and seed dispersal effect of three kinds of birds on Taxus cuspidata［J］.Journal of Northeast Forestry University，2016，44（1）：81-84，89.
2	刘彤，胡林林，郑红，等.天然东北红豆杉土壤种子库研究［J］.生态学报，2009，29（4）：1869-1876.
2	Liu T，Hu L L，Zheng H，et al.Researchs on soil seed bank of Japanese yew［J］.Acta Ecologica Sinica，2009，29（4）：1869-1876.
3	费永俊，雷泽湘，余昌均，等.中国红豆杉属植物的濒危原因及可持续利用对策［J］.自然资源，1997，19（5）：59-63.
3	Fei Y J，Lei Z X，Yu C J，et al.The cause for endangerment of Taxus L. and measures for its sustainable development in china ［J］.Natural Resources，1997，19（5）：59-63.
4	臧润国，董鸣，李俊清，等.典型极小种群野生植物保护与恢复技术研究［J］.生态学报，2016，36（22）：7130-7135.
4	Zang R G，Dong M，Li J Q，et al.Conservation and restoration for typical critically endangered wild plants with extremely small population［J］.Acta Ecologica Sinica，2016，36（22）：7130-7135.
5	张银，李文杰，李玲丽，等.模拟酸雨对3种红豆杉幼苗生理指标的影响［J］.西部林业科学，2020，49（1）：120-127.
5	Zhang Y，Li W J，Li L L，et al.Effects of simulated acid rain on physiological indexes of three species of Taxus seedlings［J］.Journal of West China Forestry Science，2020，49（1）：120-127.
6	刁云飞，刘延坤，刘玉龙，等.穆棱东北红豆杉种群结构特征［J］.中国科学：生命科学，2020，50（4）：391-397.
6	Diao Y F，Liu Y K，Liu Y L，et al.Population structure characteristics of Taxus cuspidata［J］.Scientia Sinica Vitae，2020，50（4）：391-397.
7	Zu Y G，Chen H F，Wang W J，et al.Population structure and distribution pattern of Taxus cuspidata in Muling region of Heilongjiang Province，China［J］.Journal of Forestry Research，2006，17（1）：80-82.
8	曹聪梅，李作平，史清文.东北红豆杉的化学成分和药理作用研究进展［J］.天然产物研究与开发，2006，18（2）：330-342，259.
8	Cao C M，Li Z P，Shi Q W.Chemical constituents in Taxus cuspidata and their bioactivities［J］.Natural Product Research and Development，2006，18（2）：330-342，259.
9	邓立新.抗癌新药紫杉醇简介［J］.化学教学，2006（1）：46-48.
9	Deng L X Brief introduction of the anti-cancer medicine Peclitaxel［J］.Education in Chemistry，2006（1）：46-48.
10	王楠楠，黄飞华.红豆杉有效成分及其药理作用研究进展［J］.浙江中医杂志，2018，53（8）：621-623.
10	Wang N N，Huang F H.Zhejiang Journal of Traditional Chinese Medicine［J］.Zhejiang Journal of Traditional Chinese Medicine，2018，53（8）：621-623.
11	汤彬，薛平，李祥，等.南方红豆杉多糖的含量测定及体外降血糖活性研究［J］.食品工业科技，2013，34（009）：128-131.
11	Tang B，Xue P，Li X，et al.Study on the determination and hypoglycemic activity in vitro of Taxus chinensis var.mairei polysaccharide［J］.Science and Technology of Food Industry，2013，34（9）：128-131，136.
12	Zhang L，Liu P Z，Li L L，et al.Identification and antioxidant activity of flavonoids extracted from Xinjiang Jujube（Ziziphus jujube Mill.） leaves with ultra-high pressure extraction technology［J］.Molecules，2019，24（1）：122.
13	Murahari M，Singh V，Chaubey P，et al.A critical review on anticancer mechanisms of natural flavonoid puerarin［J］.Anti-Cancer Agents in Medicinal Chemistry，2020，20（6）：678-686.
14	Chen Y C，Shen S C，Chow J M，et al.Flavone inhibition of tumor growth via apoptosis in vitro and in vivo［J］.International Journal of Oncology，2004，25（3）：661-670.
15	吴世雄，刘艳红，张利民，等.不同产地东北红豆杉幼苗迁地保护的生长稳定性分析［J］.北京林业大学学报，2018，40（12）：27-37.
15	Wu S X，Liu Y H，Zhang L M，et al.Growth stability analysis of ex situ conservation of Taxus cuspidata seedlings from different sources［J］.Journal of Beijing Forestry University.2018，40（12）：27-37.
16	苟晓莉，金晶，毕惠嫦，等.红豆杉枝叶提取物及紫杉烷类成分对4种肿瘤细胞的毒性作用研究［J］.中国药理学通报，2016，32（4）：591-592.
16	Gou X L，Jin J，Bi H C，et al.Study on antitumor activity of extracts of branches and leaves of Taxus chinensis and taxoids on four cell lines［J］.Chinese Pharmacological Bulletin，2016，32（4）：591-592.
17	Ketchum R E B，Horiguchi T，Qiu D Y，et al.Administering cultured Taxus cells with early precursors reveals bifurcations in the taxoid biosynthetic pathway［J］.Phytochemistry，2007，68（3）：335-341.
18	陈一鸣，王涛，张凤姣，等.南方红豆杉野生种与栽培品种‘金锡杉’针叶代谢物的比较分析［J］.西北植物学报，2019，39（5）：801-807.
18	Chen Y M，Wang T，Zhang F J，et al.Comparison of metabolites in the needles of Taxus wallichiana var.mairei and Taxus wallichiana var. mairei cv.‘Jinxishan’［J］.Acta Botanica Boreali-Occidentalia Sinica，2019，39（5）：801-807.
19	李影影，熊耀康.光照强度与南方红豆杉初生代谢产物相关性研究［J］.中华中医药杂志，2018，33（6）：2659-2664.
19	Li Y Y，Xiong Y K.Collection study between light intensity and primary metabolite content of Taxus mairei（Lemee et Levl.） S.Y.Hu ex Liu.［J］.China Journal of Traditional Chinese Medicine and Pharmacy，2018，33（6）：2659-2664.
20	张春椿，年慧慧，李效贤，等.GC/MS法对南方红豆杉种皮抑制物分析研究［J］.浙江中医药大学学报，2013，37（4）：446-450.
20	Zhang C C，Nian H H，Li X X，et al.Study on seed coat dormancy substances of Taxusmairei（Lemee et Levl.） S.Y.Hu ex Liu by GC/MS［J］.Journal of Zhejiang Chinese Medical University，2013，37（4）：446-450.
21	Kind T，Wohlgemuth G，Lee D Y，et al.FiehnLib：mass spectral and retention index libraries for metabolomics based on quadrupole and time-of-flight gas chromatography/mass spectrometry［J］.Analytical Chemistry，2009，81（24）：10038-10048.
22	Dunn W B，Broadhurst D，Begley P，et al.Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry［J］.Nature Protocols，2011，6（7）：1060-1083.
23	Jolliffe I T.Principal Component Analysis［J］.Journal of Marketing Research，2002，87（4）：513.
24	Wiklund S，Johansson E，Sj?str?m L，et al.Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models［J］.Analytical chemistry，2008，80（1）：115-122.
25	Trygg J，Wold S.Orthogonal projections to latent structures（O-PLS）［J］.Journal of Chemometrics，2002，16（3）：119-128.
26	洪森荣，吴夏俊鹏，徐文慧，等.低温离体保存黄独微型块茎转录组、蛋白质组和代谢组的关联分析［J］.浙江农业学报，2017，29（11）：1827-1834.
26	Hong S R，Wuxia J P，Xu W H，et al.Correlation analysis of transcriptome，proteome and metabolome of Dioscorea bulbifera L.microtubers conserved in vitro at low temperature［J］.Acta Agriculturae Zhejiangensis，2017，29（11）：1827-1834.
27	Kanehisa M，Goto S.KEGG：Kyoto encyclopedia of genes and genomes［J］.Nucleic Acids Research，2000，28（1）：27-30.
28	Xia J G，Sinelnikov I V，Han B，et al.MetaboAnalyst 3.0-making metabolomics more meaningful［J］.Nucleic Acids Research，2015，43（W1）：W251-W257.
29	许祥明，叶和春，李国凤.脯氨酸代谢与植物抗渗透胁迫的研究进展［J］.植物学通报，2000，17（6）：536-542.
29	Xu X M，Ye H C，Li G F.Progress in synthesis and metabolism of proline and its relationship with osmotolerance of plants［J］.Chinese Bulletin of Botany，2000，17（6）：536-542.
30	刘建兵.干旱胁迫对马尾松苗木脯氨酸及游离氨基酸含量的影响［J］.湖南林业科技，2017，44（1）：14-18.
30	Liu J B.Effect of drought stress on the contents of proline and free amino acid in masson pine seedlings［J］.Hunan Forestry Science & Technology，2017，44（1）：14-18.
31	夏海威，吴娟，黄敏.外源鸟氨酸对菹草（Potamogeton crispus L.）抗镉胁迫能力的影响［J］.湖泊科学，2014，26（02）：288-296.
31	XIA H W，W J，H M，Effects of exogenous ornithine on resistance of Potamogeton crispus L.to cadmium stress［J］，Journal of Lake Sciences，2014，26（02）：288-296.4.
32	朱虹，祖元刚，王文杰，等.逆境胁迫条件下脯氨酸对植物生长的影响［J］.东北林业大学学报，2009，37（4）：86-89.
32	Zhu H，Zu Y G，Wang W J，et al.Effect of proline on plant growth under different stress conditions［J］.Journal of Northeast Forestry University，2009，37（4）：86-89.
33	包雨卓，杨宁，苍晶，等.冬小麦东农冬麦1号在不同温度下的代谢组学差异分析［J］.麦类作物学报，2017，37（5）：647-655.
33	Bao Y Z，Yang N，Cang J，et al.Metabolomic profiling of winter wheat dongnongdongmai 1 grown at different temperatures［J］.Journal of Triticeae Crops，2017，37（5）：647-655.
34	黄骥，王建飞，张红生.植物戊糖磷酸途径及其两个关键酶的研究进展［J］.植物学通报，2004，21（2）：139-145.
34	Huang J，Wang J F，Zhang H S.Advances on plant pentose phosphate pathway and its key enzymes［J］.Chinese Bulletin of Botany，2004，21（2）：139-145.
35	朱明月，杨淼，张玮琪，等.黑龙江穆棱东北红豆杉自然保护区马鹿与狍冬季食性组成的比较［J］.东北林业大学学报，2019，47（5）：100-104.
35	Zhu M Y，Yang M，Zhang W Q，et al.Wintering diet of red and roe deer in Heilongjiang Muling Japnaese Yew National Reserve［J］.Journal of Northeast Forestry University，2019，47（5）：100-104.
36	张欣，高增平.阿魏酸的研究进展［J］.中国现代中药，2020，22（01）：138-147.
36	Zhang X，Gao Z P.Research Progress in Ferulic Acid［J］.Modern Chinese Medicine，2020，22（01）138-147.
37	方紫雯，张夏燕，陶俊，等.阿魏酸对凤丹干旱胁迫的缓解效应［J］.植物研究，2020，40（3）：353-359.
37	Fang Z W，Zhang X Y，Tao J，et al.Ameliorative effect of ferulic acid on Paeonia ostii under drought stress［J］.Bulletin of Botanical Research，2020，40（3）：353-359.

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