Tissue Culture and Rapid Propagation System of Gentiana rhodantha

doi:10.7525/j.issn.1673-5102.2021.05.014

Abstract

Abstract:

To establish the tissue culture system of Gentiana rhodantha， the proper explant， disinfecting methods， the best medium for seedling proliferation， for rooting and methods for transplanting were studied respectively. The results showed that the stems with node were the best explants， and the survival rate reached 80% with no contamination by disinfecting with 5% sodium hypochlorite for 10 min； the best medium for bud proliferation was： MS+0.1 mg·L^-1 IBA+2.5 mg·L^-1 ZT with a proliferation coefficient of 8.94， and the best rooting medium was： 1/2MS+0.3 mg·L^-1 IBA with a rooting rate of 100%； the best transplant medium was perlite∶turf=1∶1 with a survival rate up to 100%. This study established a rapid propagation system， provided the technical support for the factory seedling and large-scale cultivation of G. rhodantha.

Key words: Gentiana rhodantha, polyploid, tissue culture

CLC Number:

S682.1+9

Shi-Jun ZHONG, Bi-Dong ZHANG, Shi-Ting LAI, Hua-Min LIU. Tissue Culture and Rapid Propagation System of Gentiana rhodantha[J]. Bulletin of Botanical Research, 2021, 41(5): 753-759.

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