整合生物学期刊网

植物科学 ›› 2024, Vol. 42 ›› Issue (3): 359-365.DOI: 10.11913/PSJ.2095-0837.23259

• • 上一篇    下一篇

基于流式细胞术对观赏海棠品种的基因组大小测定

张艳艳1(), 彭冶1(), 林峰2, 杨立梅1, 张往祥3   

  1. 1 南京林业大学南方现代林业协同创新中心，生命科学学院，南京 210037
    2 南京林业大学分析测试中心，南京 210037
    3 南京林业大学林草学院、水土保持学院，南京 210037
  • 出版日期:2024-06-30 发布日期:2024-08-14

Genome size determination of ornamental crabapple cultivars based on flow cytometry

Zhang Yanyan1(), Peng Ye1(), Lin Feng2, Yang Limei1, Zhang Wangxiang3   

  1. 1 Co-Innovation Center for Sustainable Forestry in Southern China, College of Life Science, Nanjing Forestry University, Nanjing 210037, China
    2 Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China
    3 College of Forestry and Grassland, College of Soil and Water Conser Nanjing Forestry University, Nanjing 210037, China
  • Online:2024-06-30 Published:2024-08-14

RichHTML

42

PDF (PC)

104

摘要：

核DNA C值和基因组大小是重要的生物多样性特征，本研究以18个观赏海棠（Malus spp.）品种的嫩叶为材料，并以玉米（Zea maysL.）品种‘B73’为内参样本，利用流式细胞术对观赏海棠基因组大小进行了测定。结果显示：18份观赏海棠C值大小范围为0.74~1.50 pg，平均为0.96 pg；其中，‘玛丽波特’（‘Mary Potter’）C值最大，达到1.50 pg，而‘红珠宝’（‘Red Jewel’）C值最小，为0.74 pg。方差分析结果表明观赏海棠品种间C值大小存在显著差异。观赏海棠不同品种间DNA C值和基因组大小存在显著的多样性。

Abstract:

Nuclear DNA C value and genome size are important characteristics of biodiversity. In this study, young leaves of 18 cultivars of ornamental crabapple (Malus spp.) were used as test material, and Zea mays L. cv. ‘B73’ was used as an internal reference sample. The genome size of ornamental crabapple was measured by flow cytometry. It provides a theoretical basis for germplasm resource identification, genomics research and new variety breeding of ornamental crabapple. The results showed that the C value of 18 samples of ornamental crabapple ranged from 0.74 to 1.50 pg, and the average size was 0.96 pg. Among them, ‘Mary Potter’ had the highest C value (1.50 pg), while ‘Red Jewel’ had the lowest C value (0.74 pg). ANOVA showed that there were significant differences in the C value between ornamental begonia cultivars. The research results indicate significant diversity in DNA C values and genome sizes among different ornamental crabapple cultivars.

版权所有 © 《植物科学》编辑部
技术支持：北京玛格泰克科技发展有限公司