A new infrageneric classification of Gastrochilus (Orchidaceae: Epidendroideae) based on molecular and morphological data

doi:10.1016/j.pld.2023.08.001

Plant Diversity ›› 2024, Vol. 46 ›› Issue (04): 435-447.DOI: 10.1016/j.pld.2023.08.001

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A new infrageneric classification of Gastrochilus (Orchidaceae: Epidendroideae) based on molecular and morphological data

Jun-Yi Zhang^a,b, Yue-Hong Cheng^c, Min Liao^a,d, Yu Feng^a, Sen-Long Jin^c, Ting-Mei He^c, Hai He^b, Bo Xu^a,d

a. China-Croatia “Belt and Road” Joint Laboratory on Biodiversity and Ecosystem Services, CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, P. O. Box 416, Chengdu 610041, Sichuan, China;
b. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;
c. Wolong National Natural Reserve Administration Bureau, Wenchuan 623006, Sichuan, China;
d. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2022-12-09 Revised:2023-08-09 Online:2024-07-29 Published:2024-07-25
Contact: Hai He,E-mail:hehai@cqnu.edu.cn;Bo Xu,E-mail:xubo@cib.ac.cn
Supported by:
This study was supported by the National Key Research and Development Program of China (Grant No. 2020YFE0203200), the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (Grant Nos. 2019QZKK0301 & 2019QZKK0502), 2022 Central Finance Forestry Grassland Ecological Protection and Restoration National Park Subsidy Project, 2022–2023 Subsidy Projects of Prohibited Developmental Areas from the Transfer Payment of the National Key Ecological Functional Areas, 2023 Central financial protection and restoration funds for forestry and grassland ecology, and Wild Plants Sharing and Service Platform of Sichuan Province.

A new infrageneric classification of Gastrochilus (Orchidaceae: Epidendroideae) based on molecular and morphological data

Jun-Yi Zhang^a,b, Yue-Hong Cheng^c, Min Liao^a,d, Yu Feng^a, Sen-Long Jin^c, Ting-Mei He^c, Hai He^b, Bo Xu^a,d

a. China-Croatia “Belt and Road” Joint Laboratory on Biodiversity and Ecosystem Services, CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, P. O. Box 416, Chengdu 610041, Sichuan, China;
b. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;
c. Wolong National Natural Reserve Administration Bureau, Wenchuan 623006, Sichuan, China;
d. University of Chinese Academy of Sciences, Beijing 100049, China

通讯作者: Hai He,E-mail:hehai@cqnu.edu.cn;Bo Xu,E-mail:xubo@cib.ac.cn
基金资助:
This study was supported by the National Key Research and Development Program of China (Grant No. 2020YFE0203200), the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (Grant Nos. 2019QZKK0301 & 2019QZKK0502), 2022 Central Finance Forestry Grassland Ecological Protection and Restoration National Park Subsidy Project, 2022–2023 Subsidy Projects of Prohibited Developmental Areas from the Transfer Payment of the National Key Ecological Functional Areas, 2023 Central financial protection and restoration funds for forestry and grassland ecology, and Wild Plants Sharing and Service Platform of Sichuan Province.

Abstract

Abstract: Gastrochilus is an orchid genus containing 73 species of mainly epiphytic on trees or rocks in mountain forests of tropical and subtropical Asia. Previous phylogenetic analyses and morphological assessments have failed to produce a well-resolved phylogeny at the infrageneric level. In the present study, a new infrageneric classification of Gastrochilus is proposed based on thoroughly morphological and phylogenetic analyses based on 52 species. Our phylogenetic analysis divided the genus into six sections including three new sections, G. sect. Pseudodistichi, G. sect. Brachycaules and G. sect. Acinacifolii. We also reinstate G. suavis to the specific rank. Furthermore, two new species, G. armeniacus Jun Y. Zhang, B. Xu & Yue H. Cheng and G. minjiangensis Jun Y. Zhang, B. Xu & Yue H. Cheng, are described and illustrated. A key to six sections of the genus is presented.

Key words: Gastrochilus, Phylogeny, Character evolution, Taxonomy, New species

摘要： Gastrochilus is an orchid genus containing 73 species of mainly epiphytic on trees or rocks in mountain forests of tropical and subtropical Asia. Previous phylogenetic analyses and morphological assessments have failed to produce a well-resolved phylogeny at the infrageneric level. In the present study, a new infrageneric classification of Gastrochilus is proposed based on thoroughly morphological and phylogenetic analyses based on 52 species. Our phylogenetic analysis divided the genus into six sections including three new sections, G. sect. Pseudodistichi, G. sect. Brachycaules and G. sect. Acinacifolii. We also reinstate G. suavis to the specific rank. Furthermore, two new species, G. armeniacus Jun Y. Zhang, B. Xu & Yue H. Cheng and G. minjiangensis Jun Y. Zhang, B. Xu & Yue H. Cheng, are described and illustrated. A key to six sections of the genus is presented.

关键词: Gastrochilus, Phylogeny, Character evolution, Taxonomy, New species

Jun-Yi Zhang, Yue-Hong Cheng, Min Liao, Yu Feng, Sen-Long Jin, Ting-Mei He, Hai He, Bo Xu. A new infrageneric classification of Gastrochilus (Orchidaceae: Epidendroideae) based on molecular and morphological data[J]. Plant Diversity, 2024, 46(04): 435-447.

References

Beentje, H., 2012. The Kew Plant Glossary, an illustrated dictionary of plant terms. Kew Publishing, London, p. 164.
Bentham, G., Hooker, J.D., 1883. Genera Platarum. London, vol. 3, p. 579.
Bhattacharjee. A., Agrawala, D.K., Jalal, J.S., et al., 2021. Three new synonyms in Gastrochilus (Orchidaceae) with notes on typification of Gastrochilus calceolaris and misreport of Gastrochilus changjiangensis from India. Bot. Lett. 169, 106-118. https://doi.org/10.1080/23818107.2021.2000889.
Chen, S.C., Tsi, Z.H., Wood, J.J., 2009. Gastrochilus D. Don. In: Wu, Z.Y., Raven, P.H., Hong, D.Y. (Eds). Flora of China vol. 25 (Orchidaceae). Missouri Botanical Garden Press, Science Press and St. Louis, Beijing, pp. 491-498.
Chen, W.S., Lei, M., Ma, C.B., et al., 2022. Gastrochilus xizangensis (Aeridinae, Vandeae, Orchidaceae), a new species from Xizang, China. Phytotaxa 566, 219-226. https://doi.org/10.11646/phytotaxa.566.2.6.
Darlu, P., Lecointre, G., 2002. When does the incongruence length difference test fail? Mol. Biol. Evol. 19, 432-437. https://doi.org/10.1093/oxfordjournals.molbev.a004098.
Don, D., 1825. Prodromus Florae Nepalensis. J. Gale, London, p. 256.
Govaerts, R., Campacci, M.A., Baptista, D.H., et al., 2021. World Checklist of Orchidaceae. The board of trustees of the Royal Botanic Gardens, Kew. http://www.kew.org/wcsp/monocots/.
Guindon, S., Dufayard, J.F., Lefort, V., et al., 2010. New algorithms and methods to estimate Maximum-Likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59, 307-321. https://doi.org/10.1093/sysbio/syq010.
Hooker, J.D., 1890. Flora of British India, vol. 6. L. Reeve, London.
IUCN., 2022. Guidelines for using the IUCN Red List Categories and Criteria, prepared by the Standards and Petitions Committee, version 15. http://www.iucnredlist.org/documents/RedListGuidelines.pdf.
Jin, X.H., Dai, Z.Q., Liu, Q.Y., et al., 2010. Miscellaneous taxonomic notes on Orchidaceae from China. Acta Botanica Yunnan. 32, 331-333.
Jin, X.H., Ye, D.P., Li, J.W., 2019. Atlas of native orchids in China. Henan Science and Technology Press, Zhengzhou, China, p. 1080.
Johnson, M.G., Pokorny, L., Dodsworth, S., et al., 2019. A universal probe set for targeted sequencing of 353 nuclear genes from any flowering plant designed using k-Medoids Clustering. Syst Biol. 68, 594-606. https://doi.org/10.1093/sysbio/syy086.
Katoh, K., Standley, D.M., 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30, 772-780. https://doi.org/10.1093/molbev/mst010.
Kim, C., Choi, H.K., 2011. Molecular systematics and character evolution of Typha (Typhaceae) inferred from nuclear and plastid DNA sequence data. Taxon 60,1417-1428. https://doi.org/10.1002/tax.605017.
King, G., Pantling, R., 1898. The Orchids of Sikkim Himalaya. Annal Royal Botanical Garden Calcutta 8, 217-229.
Kumar, P., Gale, S.W., Kocyan, A., et al., 2014. Gastrochilus kadooriei (Orchidaceae), a new species from Hong Kong, with notes on allied taxa in section Microphyllae found in the region. Phytotaxa 164, 91-103. https://doi.org/10.11646/phytotaxa.164.2.3.
Li, J.W., Ya, J.D., Ye, D.P., et al., 2021. Taxonomy notes on Vandeae (Orchidaceae) from China: Five new species and two new records. Plant Divers. 43, 379-389. https://doi.org/10.1016/j.pld.2021.01.009.
Li, Y., Jin, W.T., Zhang, L.G., et al., 2022. Biogeography and diversification of the tropical and subtropical Asian genus Gastrochilus (Orchidaceae, Aeridinae). Diversity 14, 396. https://doi.org/10.3390/d14050396.
Liao, M., Cheng, Y.H., Zhang, J.Y., et al., 2022. Gastrochilus heminii (Orchidaceae, Epidendroideae), a new species from Sichuan, China, based on molecular and morphological data. PhytoKeys 215, 95-106. https://doi.org/10.3897/phytokeys.215.91061.
Lindley, J., 1833. The genera and species of Orchidaceous plants. Ridgeways, London, pp. 220-225.
Liu, D.K., Tu, X.D., Zhao, Z., et al., 2020. Plastid phylogenomic data yield new and robust insights into the phylogeny of Cleisostoma - Gastrochilus clades (Orchidaceae, Aeridinae). Mol. Phylogenet. Evol. 145, 106729. https://doi.org/10.1016/j.ympev.2019.106729.
Liu, Q., 2017. Taxonomic revision study of Gastrochilus D. Don (Orchidaceae) in China. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Yunnan, China, pp. 1-145.
Liu, Q., Gao, J.Y., 2018. Gastrochilus dulongjiangensis (Aeridinae, Vandeae, Epidendroideae, Orchidaceae), a new species from Yunnan Province, China. Phytotaxa 340, 293-296. https://doi.org/10.11646/phytotaxa.340.3.11.
Liu, Q., Kumar, P., Gao, J.Y., 2019b. Note on Gastrochilus gongshanensis (Orchidaceae). Kew Bulletin 74, 21. https://doi.org/10.1007/s12225-019-9808-3.
Liu, Q., Song, Y., Jin, X.H., et al., 2019a. Phylogenetic relationships of Gastrochilus (Orchidaceae) based on nuclear and plastid DNA data. Bot. J. Linn. Soc. 189, 228-243. https://doi.org/10.1093/botlinnean/boy084.
Liu, Q., Tan, Y.H., Gao, J.Y., 2016. A new species of Gastrochilus (Aeridinae, Vandeae, Orchidaceae) and a new record species from Yunnan, China. Phytotaxa 282, 066-070. https://doi.org/10.11646/phytotaxa.282.1.8.
Liu, Q., Wu, X.F., Zhou, S.S., et al., 2023. New species and record of Gastrochilus (Orchidaceae, Aeridinae) from China and Laos. Phytotaxa 585, 210-224. https://doi.org/10.11646/PHYTOTAXA.585.3.3.
Luning, B., 1967. Studies on Orchidaceae alkaloids IV. Screening of species for alkaloids 2. Phytochemistry 6, 857-861.
Maddison, W.P., Maddison, D.R., 2011. Mesquite: A Modular System for Evolutionary Analysis, version 2.75. https://mesquite.project.org.
Minh, B.Q., Nguyen, M.A.T., von-Haeseler, A., 2013. Ultrafast approximation for phylogenetic bootstrap. Mol. Biol. Evol. 30, 1188-1195. https://doi.org/10.1093/molbev/mst024.
Nguyen, L.T., Schmidt, H.A., Von-Haeseler, A., et al., 2014. IQ-TREE: a fast and effective stochastic algorithm for estimating Maximum-Likelihood phylogenies. Mol. Biol. Evol. 32, 268-274. https://doi.org/10.1093/molbev/msu300.
Nguyen, V.C., Averyanov, L.V., Maisak, T.V., et al., 2022. Gastrochilus pankajkumarii, (Aeridinae, Epidendroideae, Orchidaceae) a new lithophytic orchid from southern Vietnam. Taiwania 67, 35-39. https://doi.org/10.6165/tai.2022.67.35.
Posada, D., 2008. jModelTest: phylogenetic model averaging. Mol. Biol. Evol. 25, 1253-1256. https://doi.org/10.1093/molbev/msn083.
Pridgeon, A.M., Cribb, P.J., Chase, M.W., et al., 2014. Genera orchidacearum: Epidendroideae, volume 6, Part 3. Oxford University Press, Oxford, p. 544.
Rao, W.H., Liu, Z.J., Zhang, G.Q., et al., 2019. A new epiphytic species of Gastrochilus (Orchidaceae: Epidendroideae) from Yunnan, China. Phytotaxa 413, 296-300. https://doi.org/10.11646/phytotaxa.413.4.5.
Raskoti, B.B., 2016. A new species of Gastrochilus and new records for the orchids of Nepal. Phytotaxa 233, 179-184. https://doi.org/10.11646/phytotaxa.233.2.5.
Ronquist, F., Huelsenbeck, J.P., 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572-1574. https://doi.org/10.1093/bioinformatics/btg180.
Schlechter, F.R.R., 1913. Die Gattungen Gastrochilus Don. Und Gastrochilus Wall. Repertorium specierum novarum regni vegetabilis. Selbstverlag des Herausgebers, Berlin, pp. 313-315.
Seidenfaden, G., 1988. Orchid genera in Thailand XIV. Fifty-nine vandoid genera. Opera Botanica 95, 1-398.
Smith, J.J., 1905. Die Orchideen von Java. Flora von Buitenzorg 6, 632.
Swofford, D.L., 2002. PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0 a169. Sinauer Associates, Sunderland.
Thiers, B., 2021. Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/science/ih.
Tsi, Z.H., 1996. A preliminary revision of Gastrochilus (Orchidaceae). Guihaia 16, 123-154.
Turland, N.J., Wiersema, J.H., Barrie, F.R., et al., (2018). International Code of Nomenclature for algae, fungi, and plants (Shenzhen Code) adopted by the Nineteenth International Botanical Congress Shenzhen, China, July 2017. Regnum Vegetabile 159, Koeltz Botanical Books, Glashutten. https://doi.org/10.12705/Code.2018.
Wu, X.F., Ye, D.P., Pan, B., et al., 2019. Validation of Gastrochilus prionophyllus (Vandeae, Orchidaceae), a new species from Yunnan Province, China. In: Cai, J., Yu, W.B., Zhang, T., Li, D.Z. (Eds). Revealing of the plant diversity in China’s biodiversity hotspots. PhytoKeys 130, 161-169. https://doi.org/10.3897/phytokeys.130.34555.
Xu, Z.H., Jiang, H., Ye, D.P., et al., 2010. The wild Orchids in Yunnan. Yunnan Science and Technology Press, Kunming, p. 496.
Zhang, J.Y., Cheng, Y.H., Liao, M., et al., 2022a. Gastrochilus wolongensis (Orchidaceae): a new species from Sichuan, China, based on molecular and morphological data. Ecosyst. Health Sust. 8, 2101546. https://doi.org/10.1080/20964129.2022.2101546.
Zhang, Z., Xie, P.L., Guo, Y.L., et al., 2022b. Easy353: a tool to get angiosperms353 genes for phylogenomic research. Mol. Biol. Evol. 39, msac261. https://doi.org/10.1093/molbev/msac261.
Zhou, Z.H., Shi, R.H., Zhang, Y., et al., 2021a. Orchid diversity in China: recent discoveries. Plant Divers. 43, 341-342. https://doi.org/10.1016/j.pld.2021.07.004.
Zhou, Z.H., Shi, R.H., Zhang, Y., et al., 2021b. Orchid conservation in China from 2000 to 2020: Achievements and perspectives. Plant Divers. 43, 343-349. https://doi.org/10.1016/j.pld.2021.06.003.
Zou, L.H., Huang, J.X., Zhang, G.Q., et al., 2015. A molecular phylogeny of Aeridinae (Orchidaceae: Epidendroideae) inferred from multiple nuclear and chloroplast regions. Mol. Phylogenet. Evol. 85, 247-254. https://doi.org/10.1016/j.ympev.2015.02.014.

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A new infrageneric classification of Gastrochilus (Orchidaceae: Epidendroideae) based on molecular and morphological data

A new infrageneric classification of Gastrochilus (Orchidaceae: Epidendroideae) based on molecular and morphological data

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